Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	522,295	C→T	A627V (GCA→GTA)	ybbP →	putative ABC transporter membrane subunit YbbP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	522,295	0	C	T	100.0%	90.7 / NA	29	A627V (GCA→GTA)	ybbP	putative ABC transporter membrane subunit YbbP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/14);  total (15/14)

TAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGCATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGG  >  NC_000913/522163‑522423                                                                                                                                     |                                                                                                                                 tAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGAc                                                                                                                           >  2:550611/1‑140 (MQ=255)  aaaCGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACg                                                                                                                          <  1:43845/140‑1 (MQ=255)    aCGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACggg                                                                                                                        <  1:550611/140‑1 (MQ=255)         gCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAAcc                                                                                                                   <  1:1123158/140‑1 (MQ=255)                         tGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCg                                                                                                                  >  2:137109/1‑125 (MQ=255)                         tGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCg                                                                                                                  <  1:137109/125‑1 (MQ=255)                                      cGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGc                                                                                          >  2:462875/1‑136 (MQ=255)                                      cGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGc                                                                                          <  1:462875/136‑1 (MQ=255)                                      cGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTggg                                                                                      >  2:623570/1‑140 (MQ=255)                                                   ttCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACgg                                                                         >  2:136034/1‑140 (MQ=255)                                                   ttCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACgg                                                                         >  2:727663/1‑140 (MQ=255)                                                   ttCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACgg                                                                         <  1:623570/140‑1 (MQ=255)                                                     ccGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCa                                                                       >  2:1119750/1‑140 (MQ=255)                                                        cgcTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGt                                                                    <  2:520950/140‑1 (MQ=255)                                                        cgcTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCAGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGt                                                                    >  1:323849/1‑140 (MQ=255)                                                           tAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGa                                                                 <  2:487638/140‑1 (MQ=255)                                                                         tGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGc                                                   >  2:271012/1‑140 (MQ=255)                                                                                 gTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTCCCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTccc                                           <  1:467108/140‑1 (MQ=255)                                                                                          gAAAGTCTGCGGCTTATTTTCTATTTTTTTTTCCCTGAGGGGGTATTAGACGGGCACCCGAAGGGCTGGCTTACCATTTTCCGCTGGGAGAATGGCACCGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAg                                  <  2:524825/140‑1 (MQ=255)                                                                                          gAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAg                                  <  1:1119750/140‑1 (MQ=255)                                                                                          aaaaGTCTGCGCCCTAATTTTTATTTTATTTCCCCGGAAGGGGTATTACGCGGGCACCCGCAGAGCGGGCTTACCATTTTCCGCTGGGAGAATGGCAAGGTCATGTTGACACAACTCATCCGCCAGTTCCCGACCATTAg                                  <  2:772765/139‑1 (MQ=255)                                                                                            aaGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCACCAGTTCCCGACCATTAGcc                                <  1:827342/140‑1 (MQ=255)                                                                                              gTCTGCGGCCTAATTTCTATTTTTTTTTCCCTGAAGGGGTATTAGACGGGCACCCGCAGAGCTGGTTTACAAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTg                              <  1:603316/140‑1 (MQ=255)                                                                                              gTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTg                              >  1:1055464/1‑140 (MQ=255)                                                                                                 tGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCAt                                     <  2:489136/130‑1 (MQ=255)                                                                                                 tGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCAt                                     >  1:489136/1‑130 (MQ=255)                                                                                                   cGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTa                                   <  1:655954/130‑1 (MQ=255)                                                                                                   cGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTa                                   >  2:655954/1‑130 (MQ=255)                                                                                                   cGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGa                         >  1:482552/1‑140 (MQ=255)                                                                                                         aaTTTCTATTTTATTTTCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTgg                   >  2:1043343/1‑140 (MQ=255)                                                                                                                         tCCCTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAg   >  1:751866/1‑140 (MQ=255)                                                                                                                          cccTGAAGGGGTATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAgg  <  2:751866/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                 TAAACGTTGCCCTCGGTGATACCGTGACTTTTATGGGCGATACCCAGGAGTTCCGCGCTAAAGTGACCAGCCTGCGCAAAGTGGACTGGGAAAGTCTGCGGCCTAATTTCTATTTTATTTTCCCTGAAGGGGCATTAGACGGGCAACCGCAGAGCTGGCTTACCAGTTTCCGCTGGGAGAATGGCAACGGCATGTTGACACAACTCAACCGCCAGTTCCCGACCATTAGCCTGTTAGATATTGGCGCGATTTTAAAACAGG  >  NC_000913/522163‑522423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑