Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	556,864	A→G	F4L (TTT→CTT)	ybcJ ←	putative RNA‑binding protein YbcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	556,864	0	A	G	100.0%	197.5 / NA	54	F4L (TTT→CTT)	ybcJ	putative RNA‑binding protein YbcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/38);  total (16/38)

TTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAATGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCG  >  NC_000913/556727‑556996                                                                                                                                          |                                                                                                                                     tttGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTg                                                                                                                                    >  2:1027382/1‑140 (MQ=255)  ttGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAgtgt                                                                                                                                   <  1:663468/140‑1 (MQ=255)    gcgcGTTTCATCCGCACCGTCGACTTTCACCTTGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCg                                                                                                                                 <  2:800799/140‑1 (MQ=255)    gcgcGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCg                                                                                                                                 <  1:220139/140‑1 (MQ=255)    gcgcGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCg                                                                                                                                 <  1:514699/140‑1 (MQ=255)    gcgcGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCg                                                                                                                                 <  1:537572/140‑1 (MQ=255)    gcgcGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCg                                                                                                                                 <  2:460865/140‑1 (MQ=255)      gcgTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGcc                                                                                                                               <  2:1159243/140‑1 (MQ=255)       cgTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCa                                                                                                                              <  1:1144646/140‑1 (MQ=255)       cgTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCa                                                                                                                              <  1:1027382/140‑1 (MQ=255)        gTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCAt                                                                                                                             <  1:298111/140‑1 (MQ=255)        gTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCAt                                                                                                                             <  2:83440/140‑1 (MQ=255)               ccGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTc                                                                                                                      <  1:692657/140‑1 (MQ=255)                  cACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCa                                                                                                                              >  2:1046668/1‑129 (MQ=255)                  cACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCa                                                                                                                              <  1:1046668/129‑1 (MQ=255)                  cACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATc                                                                                                                   >  2:699606/1‑140 (MQ=255)                  cACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATc                                                                                                                   >  1:968966/1‑140 (MQ=255)                   aCCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATcc                                                                                                                  <  2:968966/140‑1 (MQ=255)                    ccGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCt                                                                                                                 >  1:1034820/1‑140 (MQ=255)                     cGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCtg                                                                                                                <  1:1009742/140‑1 (MQ=255)                       tCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACGGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCtgtg                                                                                                              <  2:874753/140‑1 (MQ=255)                       tCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCtgtg                                                                                                              >  2:1120848/1‑140 (MQ=255)                       tCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCtgtg                                                                                                              <  1:399588/140‑1 (MQ=255)                         gACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGa                                                                                                            <  2:1092866/140‑1 (MQ=255)                         gACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCACCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGa                                                                                                            <  1:350917/140‑1 (MQ=255)                               cACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCt                                                                                                                 >  1:765665/1‑129 (MQ=255)                               cACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCt                                                                                                                 <  2:765665/129‑1 (MQ=255)                                        ttCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAacg                                                                                             <  2:1034820/140‑1 (MQ=255)                                          cGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAacgca                                                                                           >  1:385000/1‑140 (MQ=255)                                          cGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAacgca                                                                                           <  2:860/140‑1 (MQ=255)                                          cGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAacgca                                                                                           >  1:1064402/1‑140 (MQ=255)                                          cGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAacgca                                                                                           <  2:677838/140‑1 (MQ=255)                                          cGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAacgca                                                                                           <  2:385000/140‑1 (MQ=255)                                          cGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAacgca                                                                                           <  1:800191/140‑1 (MQ=255)                                          cGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAacgca                                                                                           >  1:929219/1‑140 (MQ=255)                                                 cgcgATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGt                                                                                    <  1:1120848/140‑1 (MQ=255)                                                   cgATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAg                                                                                  <  1:309528/140‑1 (MQ=255)                                                     aTTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCg                                                                                <  1:865850/140‑1 (MQ=255)                                                                cgcgcCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATc                                                                     >  1:824226/1‑140 (MQ=255)                                                                  cgccgcTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAg                                                                   <  2:305753/140‑1 (MQ=255)                                                                    ccgcTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCg                                                                 >  2:127931/1‑140 (MQ=255)                                                                         ttCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCa                                                            <  1:52662/140‑1 (MQ=255)                                                                                         aGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCaa                                            <  2:824226/140‑1 (MQ=255)                                                                                           tttCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAAcg                                          <  2:392649/140‑1 (MQ=255)                                                                                                     tCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTTGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGaa                                <  1:10351/140‑1 (MQ=255)                                                                                                      cGCACAGCTCAACGTGCGCATGTTTACAAAAAGAAAGTGCCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGCAAc                               <  2:879748/140‑1 (MQ=255)                                                                                                          caGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGc                           <  1:617177/140‑1 (MQ=255)                                                                                                          caGCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGc                           <  1:314009/140‑1 (MQ=255)                                                                                                            gCTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGt                         <  2:549445/140‑1 (MQ=255)                                                                                                             cTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTa                        <  1:915116/140‑1 (MQ=255)                                                                                                             cTCAACGTGCGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTa                        >  1:299095/1‑140 (MQ=255)                                                                                                                      cGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCg                          >  1:95086/1‑129 (MQ=255)                                                                                                                      cGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCg                          <  2:95086/129‑1 (MQ=255)                                                                                                                      cGGATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAg               <  1:969275/140‑1 (MQ=255)                                                                                                                       ggATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAgg              >  2:589241/1‑140 (MQ=255)                                                                                                                       ggATGTTTACCTAAAGAAAGTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAgg              >  1:583663/1‑140 (MQ=255)                                                                                                                                   aaagaaagTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCcg  <  2:711164/140‑1 (MQ=255)                                                                                                                                   aaagaaagTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCcg  >  1:1135616/1‑140 (MQ=255)                                                                                                                                   aaagaaagTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCcg  <  2:1064402/140‑1 (MQ=255)                                                                                                                                   aaagaaagTGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCcg  >  1:603963/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     TTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGCGCGCCGCTTTCGCTCCAGCCTTCCAGTTTCAGCAAGTCGCACAGCTCAACGTGCGGATGTTTACCTAAAGAAAATGTCGCCATGTTACTCATCCTGTGGATCATGATATTCAACGCACGCCTGTAGCGTGTTTTCAATCAGCGTGGCAACCGTCATCGGGCCAACGCCGCCGGGAACAGGCGTAATGTATGAGGCGCGTTTAGCCG  >  NC_000913/556727‑556996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑