Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	684,726	T→C	D177G (GAC→GGC)	gltL ←	glutamate/aspartate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	684,726	0	T	C	100.0%	188.7 / NA	52	D177G (GAC→GGC)	gltL	glutamate/aspartate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

AGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGA  >  NC_000913/684590‑684857                                                                                                                                         |                                                                                                                                    aGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCa                                                                                                                                  >  2:688168/1‑140 (MQ=255)   cGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGt                                                                                                                                >  1:211757/1‑140 (MQ=255)   cGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGt                                                                                                                                >  2:630888/1‑140 (MQ=255)      cTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACt                                                                                                                             >  2:677984/1‑140 (MQ=255)         tCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCg                                                                                                                          <  1:131255/140‑1 (MQ=255)             cGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGa                                                                                                                      <  1:688168/140‑1 (MQ=255)                           ttttACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATcc                                                                                                        >  2:436740/1‑140 (MQ=255)                           ttttACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATcc                                                                                                        >  2:780617/1‑140 (MQ=255)                                     gTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATg                                                                                              >  2:749690/1‑140 (MQ=255)                                     gTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATg                                                                                              >  2:598678/1‑140 (MQ=255)                                      tCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGt                                                                                             >  1:1105562/1‑140 (MQ=255)                                        cATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCg                                                                                           <  2:1124166/140‑1 (MQ=255)                                           aaaGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGtt                                                                                        <  1:749690/140‑1 (MQ=255)                                               aTCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTc                                                                                    >  1:379440/1‑140 (MQ=255)                                                  aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                                 >  2:229065/1‑140 (MQ=255)                                                  aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                                 >  2:447167/1‑140 (MQ=255)                                                  aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                                 >  1:258340/1‑140 (MQ=255)                                                  aCCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCaaa                                                                                 >  1:877985/1‑140 (MQ=255)                                                   cccGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAAc                                                                                >  2:779267/1‑140 (MQ=255)                                                       aTTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCa                                                                            <  1:598678/140‑1 (MQ=255)                                                        ttCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCAt                                                                           <  1:891416/140‑1 (MQ=255)                                                         tCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATc                                                                          >  2:909669/1‑140 (MQ=255)                                                             cACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCaa                                                                      <  2:1105562/140‑1 (MQ=255)                                                              aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                                     <  2:1063717/140‑1 (MQ=255)                                                              aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                                     <  1:447167/140‑1 (MQ=255)                                                              aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                                     <  1:630888/140‑1 (MQ=255)                                                              aCTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAAt                                                                     <  2:340275/140‑1 (MQ=255)                                                                tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  1:780617/140‑1 (MQ=255)                                                                tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  1:256296/140‑1 (MQ=255)                                                                tttACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  1:335605/140‑1 (MQ=255)                                                                       gCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   >  1:696363/1‑133 (MQ=255)                                                                       gCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  2:696363/133‑1 (MQ=255)                                                                           aGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATacac                                                        >  2:189056/1‑140 (MQ=255)                                                                             cccATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAGCAGCATCGCAATAGGATCCATACACaa                                                      <  2:814599/140‑1 (MQ=255)                                                                                 tttCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   >  2:24289/1‑123 (MQ=255)                                                                                 tttCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAg                                                                   <  1:24289/123‑1 (MQ=255)                                                                                    cGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCgcga                                               <  2:877985/140‑1 (MQ=255)                                                                                        ggTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCgcgagcga                                           <  2:379440/140‑1 (MQ=255)                                                                                           caccacCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTg                                        <  1:982280/140‑1 (MQ=255)                                                                                            accaccATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGc                                       >  1:418863/1‑140 (MQ=255)                                                                                                   tcatGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACgc                                >  2:1061671/1‑140 (MQ=255)                                                                                                   tcatGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACgc                                >  2:72212/1‑140 (MQ=255)                                                                                                        gTCATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACgctgctg                           >  2:379955/1‑140 (MQ=255)                                                                                                          cATTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACgctgctgct                         >  1:762204/1‑140 (MQ=255)                                                                                                           aTTCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGATTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGctgctgctg                        >  2:1015104/1‑140 (MQ=255)                                                                                                             tCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAc                      <  1:1166794/140‑1 (MQ=255)                                                                                                             tCCTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAc                      >  2:1166794/1‑140 (MQ=255)                                                                                                               cTTCGTTCGCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAccg                    >  2:664281/1‑140 (MQ=255)                                                                                                                ttcgttcgCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAccgc                   <  2:211757/140‑1 (MQ=255)                                                                                                                 tcgttcgCCAGTTCCACCATCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGAccgcc                  >  1:95428/1‑140 (MQ=255)                                                                                                                                accaTCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCgg   <  2:95428/140‑1 (MQ=255)                                                                                                                                 ccaTCACGCCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGa  >  1:306870/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    AGCGTCTTTCGGCGAGTCTTCGACAATTTTACCCTCGTCCATAAAGATCACCCGATTCGCCACTTTACGGGCAAAGCCCATTTCGTGGGTCACCACCATCATGGTCATTCCTTCGTTCGCCAGTTCCACCATCACGTCCAGTACTTCGTTGATCATCTCCGGATCCAGCGCCGATGTCGGTTCGTCAAACAGCATCGCAATAGGATCCATACACAACGCGCGAGCGATTGCCACACGCTGCTGCTGACCGCCGGAAAGCTGCGCCGGA  >  NC_000913/684590‑684857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑