Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	719,001	T→C	N487S (AAT→AGT)	speF ←	ornithine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	719,001	0	T	C	100.0%	201.3 / NA	55	N487S (AAT→AGT)	speF	ornithine decarboxylase, degradative
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

TTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGAACGGACGA  >  NC_000913/718864‑719135                                                                                                                                          |                                                                                                                                       tttCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTTCATGGATCGACAAAGTATTGACtc                                                                                                                                      <  1:1138586/140‑1 (MQ=255)                         gcAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGc                                                                                                              <  2:1054753/140‑1 (MQ=255)                          cAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGcc                                                                                                             >  2:493147/1‑140 (MQ=255)                                 ttAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTc                                                                                                      <  2:908063/140‑1 (MQ=255)                                   aGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAgtg                                                                                                         <  2:46002/135‑1 (MQ=255)                                   aGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAgtg                                                                                                         >  1:46002/1‑135 (MQ=255)                                   aGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTccc                                                                                                    >  1:933730/1‑140 (MQ=255)                                          aTCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGcc                                                                                                             <  1:67635/124‑1 (MQ=255)                                          aTCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGcc                                                                                                             >  2:67635/1‑124 (MQ=255)                                          aTCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTAc                                                                                             >  1:332016/1‑140 (MQ=255)                                            cgtcgCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACaa                                                                                           <  2:784420/140‑1 (MQ=255)                                              tcgCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTccc                                                                                                    >  1:511365/1‑129 (MQ=255)                                              tcgCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTccc                                                                                                    <  2:511365/129‑1 (MQ=255)                                               cgcgGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACt                                                                                        >  1:673977/1‑140 (MQ=255)                                                 cgGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTgg                                                                                      >  1:1133360/1‑140 (MQ=255)                                                  gggTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGga                                                                                     <  1:493147/140‑1 (MQ=255)                                                            aaCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAg                                                                           <  1:220809/140‑1 (MQ=255)                                                            aaCGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCC‑GTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGa                                                                          >  1:136514/1‑140 (MQ=255)                                                                  tCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGAt                                                                         >  1:951871/1‑136 (MQ=255)                                                                  tCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGAt                                                                         <  2:951871/136‑1 (MQ=255)                                                                          gCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAAttt                                                             >  1:569578/1‑140 (MQ=255)                                                                            cGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTgc                                                           <  2:332016/140‑1 (MQ=255)                                                                             gTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTgcg                                                          <  1:756787/140‑1 (MQ=255)                                                                             gTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTgcg                                                          <  2:204279/140‑1 (MQ=255)                                                                               tACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCt                                                        >  1:1139928/1‑140 (MQ=255)                                                                                             ccTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTg                                          >  1:159349/1‑140 (MQ=255)                                                                                                ggggTTGTCAGCAACAGTTTGCATGGATTGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCa                                       >  1:1075566/1‑140 (MQ=255)                                                                                                ggggTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCa                                       >  1:927735/1‑140 (MQ=255)                                                                                                ggggTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCa                                       >  1:118725/1‑140 (MQ=255)                                                                                                    ttGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGt                                   <  1:697627/140‑1 (MQ=255)                                                                                                      gTCAGCAACAGTTTGCATGGATCGACGAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGttt                                 >  2:418262/1‑140 (MQ=255)                                                                                                      gTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGttt                                 >  2:676352/1‑140 (MQ=255)                                                                                                      gTCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGttt                                 >  1:79507/1‑140 (MQ=255)                                                                                                       tCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTa                                >  1:980636/1‑140 (MQ=255)                                                                                                       tCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTa                                <  1:335025/140‑1 (MQ=255)                                                                                                       tCAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTa                                >  1:673346/1‑140 (MQ=255)                                                                                                        cAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTAc                               >  1:515579/1‑140 (MQ=255)                                                                                                        cAGCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTAc                               >  1:855944/1‑140 (MQ=255)                                                                                                          gCAACAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCa                             <  2:795483/140‑1 (MQ=255)                                                                                                             aCAGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATcc                          <  2:707306/140‑1 (MQ=255)                                                                                                               aGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATccac                        <  1:683440/140‑1 (MQ=255)                                                                                                               aGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATccac                        <  2:118725/140‑1 (MQ=255)                                                                                                               aGTTTGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATccac                        <  2:933730/140‑1 (MQ=255)                                                                                                                 tttGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATccacca                      <  2:980636/140‑1 (MQ=255)                                                                                                                   tGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGtt                                  <  1:1059356/126‑1 (MQ=255)                                                                                                                   tGCATGGATCGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGtt                                  >  2:1059356/1‑126 (MQ=255)                                                                                                                            cGACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACg           <  2:79507/140‑1 (MQ=255)                                                                                                                             gACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGa          >  2:404288/1‑140 (MQ=255)                                                                                                                             gACAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGa          >  2:918012/1‑140 (MQ=255)                                                                                                                              aCAAAGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGaa         <  2:515579/140‑1 (MQ=255)                                                                                                                                aaaGTATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGAACg       <  2:927735/140‑1 (MQ=255)                                                                                                                                    tATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGaa         >  1:1152730/1‑134 (MQ=255)                                                                                                                                    tATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGaa         <  2:1152730/134‑1 (MQ=255)                                                                                                                                    tATTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGAACGGACg   <  2:673977/140‑1 (MQ=255)                                                                                                                                     aTTGACTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGAACGGACGa  >  2:914750/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TTTCCGGCACTACGCCATTTTCGCGCAGGAAGTTAGCAAGAATCGTCGCGGGTACACCGAACGCTTCATATTCGCCGTTACGTGCATCAATACCTGGGGTTGTCAGCAACAGTTTGCATGGATCGACAAAGTATTGATTCTCTGCATAGCCTTCAAAAGAATGCCAGTGTTCCCCTGGTACAAACTGGAAGAAGCGCAGATCAACCGCAATTTGCGCTGTTTCATACGACTGCCAGGGTTTACCATCCACCAGTTCAGGTACGAACGGACGA  >  NC_000913/718864‑719135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑