Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 744,027 A→G Q145Q (CAA→CAG)  pxpB → 5‑oxoprolinase component B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913744,0270AG98.2% 191.3 / ‑4.4 56Q145Q (CAA→CAG) pxpB5‑oxoprolinase component B
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (29/26);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

GTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGC  >  NC_000913/743902‑744162
                                                                                                                             |                                                                                                                                       
gtggtCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACg                                                                                                                           <  2:299485/140‑1 (MQ=255)
gtggtCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACg                                                                                                                           <  2:29550/140‑1 (MQ=255)
 tggtCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACgg                                                                                                                          >  1:352902/1‑140 (MQ=255)
           gcATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAAc                                                                                                                <  1:345855/140‑1 (MQ=255)
                   ggTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTa                                                                                                        <  2:632045/140‑1 (MQ=255)
                    gTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTAc                                                                                                       >  2:1075759/1‑140 (MQ=255)
                           gAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTcc                                                                                                >  2:317727/1‑140 (MQ=255)
                                   ggTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGtt                                                                                        <  1:1069490/140‑1 (MQ=255)
                                         tgAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGtt                                                                                        >  1:587862/1‑134 (MQ=255)
                                         tgAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGtt                                                                                        <  2:587862/134‑1 (MQ=255)
                                                  ctcctcCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATcggcg                                                                         >  2:196972/1‑140 (MQ=255)
                                                   tcctccGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATcggcgg                                                                        >  2:362939/1‑140 (MQ=255)
                                                     ctccGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGc                                                                      >  1:356150/1‑140 (MQ=255)
                                                       ccGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCg                                                                    <  2:352902/140‑1 (MQ=255)
                                                       ccGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCg                                                                    >  1:309492/1‑140 (MQ=255)
                                                       ccGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCg                                                                    <  1:317727/140‑1 (MQ=255)
                                                        cGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGc                                                                   >  2:154934/1‑140 (MQ=255)
                                                        cGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGc                                                                   >  1:414025/1‑140 (MQ=255)
                                                            gAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGAc                                                               <  2:198649/140‑1 (MQ=255)
                                                               tACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTgg                                                            >  1:175235/1‑140 (MQ=255)
                                                               tACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTgg                                                            >  2:449/1‑140 (MQ=255)
                                                                 cGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTgggg                                                          >  2:224611/1‑138 (MQ=255)
                                                                 cGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGgtg                                                          >  1:182562/1‑140 (MQ=255)
                                                                    ggTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGttt                                                       >  1:131328/1‑140 (MQ=255)
                                                                      tCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTAt                                                     >  2:731976/1‑140 (MQ=255)
                                                                       cTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATc                                                    >  1:1120373/1‑140 (MQ=255)
                                                                         ggTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCg                                                  <  2:22892/140‑1 (MQ=255)
                                                                              ttAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTggg                                             >  1:1019946/1‑139 (MQ=255)
                                                                                    tttCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGcc                                       >  2:773599/1‑140 (MQ=255)
                                                                                    tttCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGcc                                       >  2:195891/1‑140 (MQ=255)
                                                                                    tttCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGcc                                       >  1:1044544/1‑140 (MQ=255)
                                                                                             ggCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGtggctg                              <  1:195891/140‑1 (MQ=255)
                                                                                             ggCTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGtggctg                              >  1:131153/1‑140 (MQ=255)
                                                                                               cTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACGCACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTAGTGTTTATCCGCTGACACCCCGGGGtggctggc                            <  2:419695/140‑1 (MQ=255)
                                                                                               cTTCCCGTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGtggctggc                            >  1:619586/1‑140 (MQ=255)
                                                                                                     gTATCTCGGGAGTTTGCCGGAACAGCTACCCACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGa                      <  2:124890/140‑1 (MQ=255)
                                                                                                     gTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGa                      <  2:619586/140‑1 (MQ=255)
                                                                                                     gTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGa                      <  2:175235/140‑1 (MQ=255)
                                                                                                     gTATCTCGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGa                      <  2:182562/140‑1 (MQ=255)
                                                                                                        tctcGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTg                   >  1:920134/1‑140 (MQ=255)
                                                                                                         ctcGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTgg                  <  1:449/140‑1 (MQ=255)
                                                                                                          tcGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGt                 <  1:957723/140‑1 (MQ=255)
                                                                                                          tcGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGt                 <  1:196972/140‑1 (MQ=255)
                                                                                                           cGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTc                <  2:1130663/140‑1 (MQ=255)
                                                                                                           cGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTc                <  1:1018955/140‑1 (MQ=255)
                                                                                                           cGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTc                <  1:154934/140‑1 (MQ=255)
                                                                                                           cGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTc                <  1:1075759/140‑1 (MQ=255)
                                                                                                           cGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTc                <  1:1075223/140‑1 (MQ=255)
                                                                                                           cGGGAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTACGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTc                <  1:692692/140‑1 (MQ=255)
                                                                                                            gggAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGGGGCTGGCAGTTGATTGGTCa               >  2:620622/1‑140 (MQ=255)
                                                                                                             ggAGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCCGACTGGGGGTTTTCCGCTGGCAACGCCGGGGGGCTGGGAGGTGATTGGTCAt              >  2:1075223/1‑140 (MQ=255)
                                                                                                               aGTTTGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATAc            <  1:679892/140‑1 (MQ=255)
                                                                                                                 tttGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACct          <  2:309492/140‑1 (MQ=255)
                                                                                                                   tGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACctca        >  2:140054/1‑140 (MQ=255)
                                                                                                                   tGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGGTGATTGGGTATAActcc        >  1:324932/1‑139 (MQ=255)
                                                                                                                   tGCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGGGGGTGGCAGGTGGTTGGTCATACctcc        >  2:850904/1‑139 (MQ=255)
                                                                                                                    gCCGGAACAGCTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCAGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGGAACGCCGGGTGGGTGGCAGTTGATTTGTCATACctcac       >  2:324094/1‑140 (MQ=255)
                                                                                                                         aacagcTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGc  <  2:131328/140‑1 (MQ=255)
                                                                                                                         aacagcTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGc  <  1:731976/140‑1 (MQ=255)
                                                                                                                             |                                                                                                                                       
GTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGCTTACTCGTTCCGGCAGGTTCTGTCGGGATCGGCGGGCCGCAGACTGGTGTTTATCCGCTGGCAACGCCGGGTGGCTGGCAGTTGATTGGTCATACCTCACTCAGC  >  NC_000913/743902‑744162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑