| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 764,071 | A→G | N353S (AAC→AGC) | sucC → | succinyl‑CoA synthetase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 764,071 | 0 | A | G | 98.2% | 192.0 / ‑4.4 | 55 | N353S (AAC→AGC) | sucC | succinyl‑CoA synthetase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (30/24); total (31/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
TGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACC > NC_000913/763934‑764206 | tGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCg > 1:209632/1‑140 (MQ=255) gacAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGa > 2:766753/1‑140 (MQ=255) aaaGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGC‑CGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTc > 1:1100397/1‑140 (MQ=255) aaGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTc > 2:202526/1‑140 (MQ=255) gTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGC‑CGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGgc < 2:1100397/140‑1 (MQ=255) gAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGgcg < 2:299018/140‑1 (MQ=255) ttCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGaaa < 1:902836/140‑1 (MQ=255) ttCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGaaa < 1:410717/140‑1 (MQ=255) tCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGaaa < 1:466826/139‑1 (MQ=255) tCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGaaa > 2:466826/1‑139 (MQ=255) ttAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAGGTGGGTGTTAACGTACCGGTCGTGGTAAGTCCGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCt < 2:766609/140‑1 (MQ=255) cATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACa > 2:795045/1‑140 (MQ=255) aTCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAg < 2:137504/140‑1 (MQ=255) aTCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAg < 1:34510/140‑1 (MQ=255) tCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGc < 1:588919/140‑1 (MQ=255) cTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCg > 2:1064990/1‑140 (MQ=255) tCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGc > 1:523403/1‑140 (MQ=255) tCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGc > 2:289077/1‑140 (MQ=255) tCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGc < 2:209632/140‑1 (MQ=255) tCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGc < 1:766753/140‑1 (MQ=255) tCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGc < 1:301620/140‑1 (MQ=255) tCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGc > 1:864824/1‑140 (MQ=255) tCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGc < 1:1064990/140‑1 (MQ=255) tcgttGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTg < 2:563420/140‑1 (MQ=255) tGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTgcagc > 2:658774/1‑140 (MQ=255) tGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTgcagc > 2:226968/1‑140 (MQ=255) aCCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCaaaa > 2:405380/1‑140 (MQ=255) gTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTg > 1:36468/1‑131 (MQ=255) gTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTg < 2:36468/131‑1 (MQ=255) tcatcGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCt < 1:289077/140‑1 (MQ=255) tcatcGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCt < 1:658774/140‑1 (MQ=255) gcgcGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTcagcag > 1:413474/1‑140 (MQ=255) gcgcGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTcagcag > 2:405967/1‑140 (MQ=255) gcgcGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTcagcag > 1:389649/1‑140 (MQ=255) cgcgGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAgg > 2:216144/1‑140 (MQ=255) aGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCg < 1:1153155/140‑1 (MQ=255) aGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCg < 2:389649/140‑1 (MQ=255) aaGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCa < 2:110976/140‑1 (MQ=255) ggTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGc < 2:218004/134‑1 (MQ=255) ggTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGc > 1:218004/1‑134 (MQ=255) gtgtTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAg < 1:405380/140‑1 (MQ=255) tgtTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAgg > 2:1147780/1‑140 (MQ=255) tAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGa > 2:502322/1‑140 (MQ=255) gTACCGGTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCACAGCAGGTTGTTGCCGCAGTGGAGGGGAaata < 1:317665/140‑1 (MQ=255) gTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATg > 1:708108/1‑137 (MQ=255) gTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATg < 2:708108/137‑1 (MQ=255) gTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTcc > 1:161662/1‑140 (MQ=255) gTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTcc > 1:622781/1‑140 (MQ=255) gTCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTcc > 1:964743/1‑140 (MQ=255) tCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCa > 1:665736/1‑140 (MQ=255) tCGTGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCa > 1:153247/1‑140 (MQ=255) tGGTACGTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCAttt > 1:1164820/1‑140 (MQ=255) gTCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCg > 1:284666/1‑140 (MQ=255) tCTGGAAGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGa > 1:776262/1‑140 (MQ=255) aGGTAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATaaaaa < 2:1164820/140‑1 (MQ=255) tAACAGCGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAacac > 2:13838/1‑140 (MQ=255) aacagcGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACAcc < 2:284666/140‑1 (MQ=255) | TGACGACAAAGTGAAAGCCGTTCTGGTTAACATCTTCGGCGGTATCGTTCGTTGCGACCTGATCGCTGACGGTATCATCGGCGCGGTAGCAGAAGTGGGTGTTAACGTACCGGTCGTGGTACGTCTGGAAGGTAACAACGCCGAACTCGGCGCGAAGAAACTGGCTGACAGCGGCCTGAATATTATTGCAGCAAAAGGTCTGACGGATGCAGCTCAGCAGGTTGTTGCCGCAGTGGAGGGGAAATAATGTCCATTTTAATCGATAAAAACACC > NC_000913/763934‑764206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |