Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	177,992	A→G	R157R (CGT→CGC)	btuF ←	vitamin B12 ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	177,992	0	A	G	100.0%	274.3 / NA	74	R157R (CGT→CGC)	btuF	vitamin B12 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/41);  total (33/41)

GTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTC  >  NC_000913/177856‑178126                                                                                                                                         |                                                                                                                                       gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                     >  2:447959/1‑140 (MQ=255) gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                     >  2:18340/1‑140 (MQ=255) gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                     >  2:194979/1‑140 (MQ=255) gTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGt                                                                                                                                     >  2:881499/1‑140 (MQ=255)  ttCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGtt                                                                                                                                    >  2:907750/1‑140 (MQ=255)    cgcgGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGtttt                                                                                                                                  <  2:734378/140‑1 (MQ=255)     gcgGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGttttt                                                                                                                                 >  1:1143646/1‑140 (MQ=255)      cgGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGtttttt                                                                                                                                <  2:398126/140‑1 (MQ=255)       ggCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTa                                                                                                                               <  1:18340/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                             <  1:881499/140‑1 (MQ=255)         cTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAgg                                                                                                                             <  1:284867/140‑1 (MQ=255)           aaCTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGtt                                                                                                                           <  1:1003757/140‑1 (MQ=255)             cTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTa                                                                                                                         <  1:805574/140‑1 (MQ=255)                 cGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAg                                                                                                                     >  1:1027/1‑140 (MQ=255)                   gCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCa                                                                                                                   >  2:745634/1‑140 (MQ=255)                      aGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCatat                                                                                                                >  2:1021438/1‑140 (MQ=255)                      aGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCatat                                                                                                                <  1:447959/140‑1 (MQ=255)                       gggAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATAtt                                                                                                               <  1:97781/140‑1 (MQ=255)                       gggAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATAtt                                                                                                               <  1:307160/140‑1 (MQ=255)                       gggAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATAtt                                                                                                               <  1:140961/140‑1 (MQ=255)                        ggAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTg                                                                                                              >  1:515909/1‑140 (MQ=255)                         gAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTgc                                                                                                             <  1:158377/140‑1 (MQ=255)                                 cTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCaa                                                                                                     <  1:959508/140‑1 (MQ=255)                                 cTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCaa                                                                                                     <  2:483927/140‑1 (MQ=255)                                   gTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAAtt                                                                                                   <  1:645903/140‑1 (MQ=255)                                    tCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTg                                                                                                  <  2:1027/140‑1 (MQ=255)                                    tCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATAAGCATATTGCGCTTTCAATTg                                                                                                  >  1:717507/1‑140 (MQ=255)                                     cTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgc                                                                                                 <  2:1143646/140‑1 (MQ=255)                                      tttAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgcg                                                                                                >  2:969523/1‑140 (MQ=255)                                      tttAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgcg                                                                                                <  1:302306/140‑1 (MQ=255)                                      tttAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgcg                                                                                                >  1:125863/1‑140 (MQ=255)                                      ttaaaaGATGTTTACTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTgcg                                                                                                <  2:1128691/137‑1 (MQ=255)                                             aTGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTg                                                                                         <  1:907750/140‑1 (MQ=255)                                              tGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGTGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGa                                                                                        >  1:309637/1‑140 (MQ=255)                                              tGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGa                                                                                        >  1:213473/1‑140 (MQ=255)                                              tGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGa                                                                                        >  2:798785/1‑140 (MQ=255)                                               gTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGAt                                                                                       <  1:655527/140‑1 (MQ=255)                                                ttttCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATc                                                                                      >  1:916382/1‑140 (MQ=255)                                                  ttCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCca                                                                                    >  2:890869/1‑140 (MQ=255)                                                       cGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAgg                                                                               <  1:969523/140‑1 (MQ=255)                                                         ccACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATAAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGa                                                                             <  2:717507/140‑1 (MQ=255)                                                          cacaAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGAt                                                                            >  1:800938/1‑140 (MQ=255)                                                          cacaAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGAt                                                                            >  2:383556/1‑140 (MQ=255)                                                              aaCTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTgcg                                                                        <  1:977331/140‑1 (MQ=255)                                                                 ttCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCgg                                                                     >  1:199831/1‑140 (MQ=255)                                                                  tCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGc                                                                    <  2:1083336/140‑1 (MQ=255)                                                                      gTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGt                                                                <  2:931632/140‑1 (MQ=255)                                                                       tACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGtt                                                               <  2:1078037/140‑1 (MQ=255)                                                                         ccTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCg                                                             <  1:194979/140‑1 (MQ=255)                                                                         ccTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCg                                                             >  1:854522/1‑140 (MQ=255)                                                                         ccTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCg                                                             <  2:39269/140‑1 (MQ=255)                                                                                 tGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTc                                                     >  2:1159688/1‑140 (MQ=255)                                                                                        aCTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGc                                              >  2:524998/1‑140 (MQ=255)                                                                                        aCTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGc                                              >  2:528475/1‑140 (MQ=255)                                                                                        aCTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGc                                              >  2:1126886/1‑140 (MQ=255)                                                                                         ctctTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCg                                             <  1:1021438/140‑1 (MQ=255)                                                                                         ctctTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCg                                             <  1:452889/140‑1 (MQ=255)                                                                                           ctTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGa                                           <  1:745634/140‑1 (MQ=255)                                                                                            ttttCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGAc                                          <  2:943563/140‑1 (MQ=255)                                                                                            ttttCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGAc                                          <  2:601616/140‑1 (MQ=255)                                                                                                       tAAATGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGcc                               <  2:854522/140‑1 (MQ=255)                                                                                                        aaaTGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATCGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCa                              <  1:936304/140‑1 (MQ=255)                                                                                                          aTGGCGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGt                            <  1:1126886/140‑1 (MQ=255)                                                                                                              cGGATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGAc                        >  1:77035/1‑140 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGt                            >  1:208699/1‑134 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGt                            <  2:208699/134‑1 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGt                      >  2:226276/1‑140 (MQ=255)                                                                                                                gATTAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGt                      >  2:56551/1‑140 (MQ=255)                                                                                                                  ttAATGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTaa                    <  2:213473/140‑1 (MQ=255)                                                                                                                     aTGCCGAATTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGc                 >  2:398242/1‑140 (MQ=255)                                                                                                                           aaTTGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGc           >  2:216785/1‑140 (MQ=255)                                                                                                                             ttGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCaa         >  1:771146/1‑140 (MQ=255)                                                                                                                              tGCAGAAAAACGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAAt        <  1:216785/140‑1 (MQ=255)                                                                                                                                    aaaaCGCGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTc  <  1:226276/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GTTCGCGGCTAACTTGCGGCCAGGGAACCCGGCTGTCTTTAAAGATGTTTTCTCCGCCACAAACTTCGAGTACCTGGTTCTGAATCGACTCTTTTCCACTGGTAAATGGCGGATTAATGCCGAATTGCAGAAAAACACGTTTTTTAGGTTTATCAGCATATTGCGCTTTCAATTGCGCGTACTGATCCAGCAGGGATTGCGCGGCTTGTTCGGCCTTGTCTGGTTGCGGACTCCAGGGGGCCAGTTGACGTAACGCATTGGCAATTTGTTC  >  NC_000913/177856‑178126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑