Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,085,233	C→T	A81V (GCG→GTG)	phoH →	ATP‑binding protein PhoH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,085,233	0	C	T	100.0%	137.1 / NA	40	A81V (GCG→GTG)	phoH	ATP‑binding protein PhoH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/24);  total (16/24)

TCCTTTCTTTTCATGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGCGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGCCATAAGCAGCGTGAAGAAGAAT  >  NC_000913/1085096‑1085361                                                                                                                                          |                                                                                                                                 tCCTTTCTTTTCATGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCgtgt                                                                                                                                <  2:684678/140‑1 (MQ=255)     ttctttTCATGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGtga                                                                                                                            <  2:206291/140‑1 (MQ=255)     ttctttTCATGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGtga                                                                                                                            <  1:522183/140‑1 (MQ=255)      tctttTCATGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAGGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGtgat                                                                                                                           >  2:701469/1‑140 (MQ=255)      tctttTCATGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGtgat                                                                                                                           <  2:805060/140‑1 (MQ=255)      tctttTCATGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGtgat                                                                                                                           <  1:981295/140‑1 (MQ=255)          ttCATGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAAt                                                                                                                       >  1:119941/1‑140 (MQ=255)             aTGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAAt                                                                                                                       <  1:183778/137‑1 (MQ=255)             aTGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAAt                                                                                                                       >  2:183778/1‑137 (MQ=255)                    aCCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACt                                                                                                             <  2:540872/140‑1 (MQ=255)                      cGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCa                                                                                                           <  1:325058/140‑1 (MQ=255)                      cGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCa                                                                                                           <  1:66840/140‑1 (MQ=255)                          gCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGTCAACGGCGTGTGTGATGAATacaa                                                                                                                   <  1:1132875/128‑1 (MQ=255)                          gCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGTCAACGGCGTGTGTGATGAATacaa                                                                                                                   >  2:1132875/1‑128 (MQ=255)                          gCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAggg                                                                                                       >  2:413667/1‑139 (MQ=255)                           ccGTAAGCGGTGCGCGTAGCCGTCTTTCGGCATTTTATCCAAAAGCAAGCTATGGCTTACAAGGAAGCCACCCCTCTGATGTCCGTGCGCATAATCGCGCTGCGAACGGCGTGTGTGATGAATACAAACATGTCAAGGTg                                                                                                      <  2:357463/140‑1 (MQ=255)                           ccGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTg                                                                                                      <  1:79541/140‑1 (MQ=255)                           ccGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTg                                                                                                      <  1:165186/140‑1 (MQ=255)                           ccGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTg                                                                                                      <  2:903806/140‑1 (MQ=255)                            cGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGc                                                                                                     >  1:624146/1‑140 (MQ=255)                                     tGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATgg                                                                                            >  1:493355/1‑140 (MQ=255)                                      gcgcGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATggg                                                                                           >  2:818844/1‑140 (MQ=255)                                       cgcgTAGCCGTTTTTCGGCATTTTATCCAAAAGCTAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGa                                                                                          >  2:278410/1‑140 (MQ=255)                                             gCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACaa                                                                                    >  2:829691/1‑140 (MQ=255)                                              ccGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACaaa                                                                                   <  2:493355/140‑1 (MQ=255)                                              ccGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACaaa                                                                                   <  1:115250/140‑1 (MQ=255)                                               cGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACaaaa                                                                                  <  2:119941/140‑1 (MQ=255)                                                              tATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACaaa                                                                                   >  2:369356/1‑124 (MQ=255)                                                              tATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACaaa                                                                                   <  1:369356/124‑1 (MQ=255)                                                                     aggCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTcgc                                                            <  1:701469/138‑1 (MQ=255)                                                                     aaGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTcgc                                                            <  2:1112753/140‑1 (MQ=255)                                                                                 gCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCaaacgtg                                                >  2:20292/1‑136 (MQ=255)                                                                                     aCAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGGAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGc                                            >  2:809230/1‑140 (MQ=255)                                                                                                     tctGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTcgcg                            <  2:1174093/140‑1 (MQ=255)                                                                                                     tctGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTcgcg                            <  1:809230/140‑1 (MQ=255)                                                                                                     tctGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTcgcg                            <  2:934066/140‑1 (MQ=255)                                                                                                                tGCGCATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGCCATAagc                 >  2:964658/1‑140 (MQ=255)                                                                                                                   gcATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGcc                       <  2:414242/131‑1 (MQ=255)                                                                                                                   gcATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGcc                       >  1:414242/1‑131 (MQ=255)                                                                                                                    cATAATCGCGCTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGCCATAagcagtg             <  1:413667/140‑3 (MQ=255)                                                                                                                              cTGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGCCATAAGCAGCGTgaagaagaa   >  2:125213/1‑140 (MQ=255)                                                                                                                               tGCCAACGGCGTGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGCCATAAGCAGCGTGAAGAAGAAt  <  1:125213/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 TCCTTTCTTTTCATGTGTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAACTATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGCGCGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATCAAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGCCATAAGCAGCGTGAAGAAGAAT  >  NC_000913/1085096‑1085361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑