Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,177,383	A→G	M22V (ATG→GTG)	lolE →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,177,383	0	A	G	100.0%	137.9 / NA	38	M22V (ATG→GTG)	lolE	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/22);  total (16/22)

GAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCA  >  NC_000913/1177254‑1177513                                                                                                                                  |                                                                                                                                   gAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCt                                                                                                                          <  2:814290/140‑1 (MQ=255)        cAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTcc                                                                                                                   >  2:705288/1‑140 (MQ=255)         aGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCg                                                                                                                  <  2:452693/140‑1 (MQ=255)         aGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCg                                                                                                                  <  1:177733/140‑1 (MQ=255)         aGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCg                                                                                                                  <  1:725153/140‑1 (MQ=255)               aaaTGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTAttt                                                                                                            <  1:1009852/140‑1 (MQ=255)                 aTGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCt                                                                                                          <  1:650860/140‑1 (MQ=255)                    cGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCg                                                                                                                  <  2:552251/129‑1 (MQ=255)                    cGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCg                                                                                                                  >  1:552251/1‑129 (MQ=255)                    cGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGt                                                                                                                 <  2:430382/130‑1 (MQ=255)                    cGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGt                                                                                                                 >  1:430382/1‑130 (MQ=255)                    cGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTAcc                                                                                                       >  1:528477/1‑140 (MQ=255)                     gTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCa                                                                                                      >  1:1147769/1‑140 (MQ=255)                     gTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCa                                                                                                      <  2:189617/140‑1 (MQ=255)                           gtcgtcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCa                                                                                                <  2:676473/140‑1 (MQ=255)                            tcgtcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCAt                                                                                               <  1:705288/140‑1 (MQ=255)                              gtcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCa                                                                                                      <  2:411658/131‑1 (MQ=255)                              gtcTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCa                                                                                                      >  1:411658/1‑131 (MQ=255)                                          aaCTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTgg                                                                                 <  2:695214/140‑1 (MQ=255)                                               aGCCTGATGGGGGCGAAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTa                                                                            >  1:663807/1‑140 (MQ=255)                                                     aTGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGAtc                                                                      >  1:890425/1‑140 (MQ=255)                                                         gggCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTTAtcgtcg                                                                  >  2:699429/1‑140 (MQ=255)                                                          ggCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTgg                                                                                 >  1:431435/1‑124 (MQ=255)                                                          ggCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTgg                                                                                 <  2:431435/124‑1 (MQ=255)                                                                         aTGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGaa                                                  >  1:177458/1‑140 (MQ=255)                                                                         aTGCCTTTATCGTTATTAATTGGCCTGCG‑TTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAAc                                                 >  2:448577/1‑140 (MQ=255)                                                                                    gTTATTAATTGGCCTGCG‑TTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAAcg                                      <  1:448577/140‑1 (MQ=255)                                                                                       attaATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAAcgcg                                    <  2:663807/140‑1 (MQ=255)                                                                                       attaATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAAcgcg                                    >  2:706388/1‑140 (MQ=255)                                                                                              ggCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGaa                             <  2:890425/140‑1 (MQ=255)                                                                                              ggCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGaa                             <  1:706388/140‑1 (MQ=255)                                                                                                ccTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTTAACGCGAACTGaaca                           >  2:1001070/1‑140 (MQ=255)                                                                                                ccTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGaaca                           >  1:608486/1‑140 (MQ=255)                                                                                                ccTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGGGGGGGTTTTGTTTGTCGGCTTAAGGGCGGTGAACGGCTTTTAACGCGAACTGaaaa                           >  2:61895/1‑138 (MQ=255)                                                                                                       tttAGTCGCGGACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAACCGTAt                    <  1:270480/140‑1 (MQ=255)                                                                                                                 gACGGCGGCGCGGCGGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAACCGTATTCTGGCggtg          <  2:668604/140‑1 (MQ=255)                                                                                                                       ggcgcggcgGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCg    <  2:177458/140‑1 (MQ=255)                                                                                                                         cgcggcgGCGTGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAACCGTATTCTGTCGGTGGTGCCGCa  <  2:1147769/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   GAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTAATGGCGATGCCTTTATCGTTATTAATTGGCCTGCGTTTTAGTCGCGGACGGCGGCGCGGCGGCATGGTGTCGCTGATCTCCGTTATTTCTACCATTGGCATTGCCCTCGGCGTGGCGGTATTGATCGTCGGCTTAAGCGCGATGAACGGCTTTGAACGCGAACTGAACAACCGTATTCTGGCGGTGGTGCCGCA  >  NC_000913/1177254‑1177513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑