Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	208,270	A→G	I1049V (ATC→GTC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	208,270	0	A	G	100.0%	139.3 / NA	38	I1049V (ATC→GTC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/21);  total (17/21)

CGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTATCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGACAGGCAAA  >  NC_000913/208135‑208402                                                                                                                                        |                                                                                                                                     cgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGt                                                                                                                                  >  1:228743/1‑140 (MQ=255)   ggTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCa                                                                                                                                <  2:213253/140‑1 (MQ=255)        tGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGa                                                                                                                           <  2:228743/140‑1 (MQ=255)             aCCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGt                                                                                                                      <  2:991263/140‑1 (MQ=255)             aCCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGt                                                                                                                      <  1:648156/140‑1 (MQ=255)              ccAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTc                                                                                                                     >  2:935941/1‑140 (MQ=255)              ccAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTc                                                                                                                     >  2:1071639/1‑140 (MQ=255)              ccAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTc                                                                                                                     >  1:778945/1‑140 (MQ=255)                aaGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAg                                                                                                                   <  1:164886/140‑1 (MQ=255)                  gcgcGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCt                                                                                                                 <  1:806964/140‑1 (MQ=255)                       gCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTtgat                                                                                                            <  1:450611/140‑1 (MQ=255)                       gCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTtgat                                                                                                            <  1:574955/140‑1 (MQ=255)                         aaTCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTtgatga                                                                                                          >  2:1075701/1‑140 (MQ=255)                                    tATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTg                                                                                               <  2:219670/140‑1 (MQ=255)                                     aTCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTgg                                                                                              <  1:1075701/140‑1 (MQ=255)                                      tCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGt                                                                                                >  1:771710/1‑137 (MQ=255)                                      tCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGt                                                                                                <  2:771710/137‑1 (MQ=255)                                       cTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGc                                                                                            >  1:216161/1‑140 (MQ=255)                                                 gATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATTACTTCAGCGGTGGGCTTAAAATGAc                                                                                  <  2:582099/140‑1 (MQ=255)                                                         ttCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTcgcg                                                                          <  2:30204/140‑1 (MQ=255)                                                           ccGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGaa                                                                        <  2:749270/140‑1 (MQ=255)                                                           ccGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGaa                                                                        <  2:216161/140‑1 (MQ=255)                                                              ggcggcTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTg                                                                     <  2:1097594/140‑1 (MQ=255)                                                                                 gttTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGccc                                                  >  2:413352/1‑140 (MQ=255)                                                                                 gttTACTGACGCCCCGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGccc                                                  >  1:1295/1‑140 (MQ=255)                                                                                     aCTGACGCCCCGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGa                                              <  2:1295/140‑1 (MQ=255)                                                                                         aCGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGaaaaa                                          >  2:68566/1‑140 (MQ=255)                                                                                            cccTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAatat                                       >  1:609392/1‑140 (MQ=255)                                                                                                           aGCAATTGCTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCt                        >  2:258210/1‑140 (MQ=255)                                                                                                                   cTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCt                            >  1:186685/1‑128 (MQ=255)                                                                                                                   cTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCt                            <  2:186685/128‑1 (MQ=255)                                                                                                                   cTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTg                          <  2:121361/130‑1 (MQ=255)                                                                                                                   cTGGAAAAAGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTg                          >  1:121361/1‑130 (MQ=255)                                                                                                                         aaaGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGa          <  1:1071639/140‑1 (MQ=255)                                                                                                                          aaGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGAc         <  2:323703/140‑1 (MQ=255)                                                                                                                           aGACCGCATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGACa        >  2:483982/1‑140 (MQ=255)                                                                                                                                 cATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGACAGGCaaa  >  2:378228/1‑140 (MQ=255)                                                                                                                                 cATACTTGTCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGACAGGCaaa  >  1:876674/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACTTATCGTCAGCGGACAGGTCAGCTTTGATGACTTCAGCGGTGGGCTTAAAATGACCGCTCGCGAAGTGATGGATATTGACGAAGCCCGGGAAAAATATGCTCGCGGGCTTGCTATCTCGCTGACGGACAGGCAAA  >  NC_000913/208135‑208402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑