Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,539,139	C→T	S1151N (AGC→AAC)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,539,139	0	C	T	100.0%	178.6 / NA	52	S1151N (AGC→AAC)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/23);  total (29/23)

TGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGCTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAG  >  NC_000913/1539004‑1539275                                                                                                                                        |                                                                                                                                         tGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGac                                                                                                                                      >  1:57164/1‑140 (MQ=255)  gCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTAACTGGGCGTGATACATCATGGTCATGCCCGGCGGTACCCGTTGGTTGacg                                                                                                                                     <  2:1138241/140‑2 (MQ=255)    gTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGaccac                                                                                                                                   <  2:189637/140‑1 (MQ=255)      tGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACcg                                                                                                                                 >  1:422387/1‑140 (MQ=255)        gTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACcgcg                                                                                                                               >  2:1064341/1‑140 (MQ=255)        gTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACcgcg                                                                                                                               >  2:689971/1‑140 (MQ=255)              ggCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCa                                                                                                                         >  2:730614/1‑140 (MQ=255)                             ccGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTg                                                                                                          <  1:681006/140‑1 (MQ=255)                             accggTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTg                                                                                                          <  1:502096/136‑1 (MQ=255)                                aGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCg                                                                                                       <  1:689971/140‑1 (MQ=255)                                aGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCg                                                                                                       <  1:1064341/140‑1 (MQ=255)                                    aTGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGTAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTAAACGTTTGTTGTCCCCCGCGCGGGCAGTAAGCGCGTCCTTGGCGTTGa                                                                                                   <  2:62466/140‑1 (MQ=255)                                       aaTGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATa                                                                                                >  1:1028575/1‑140 (MQ=255)                                               cgcgCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGaa                                                                                                  <  2:342131/130‑1 (MQ=255)                                               cgcgCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGaa                                                                                                  >  1:342131/1‑130 (MQ=255)                                                  gcATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAg                                                                                     <  2:647709/140‑1 (MQ=255)                                                           ttACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCg                                                                            >  2:907270/1‑140 (MQ=255)                                                            tACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGa                                                                           <  1:1053720/140‑1 (MQ=255)                                                              cTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACa                                                                         >  2:993483/1‑140 (MQ=255)                                                                   gAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGt                                                                    <  2:401920/140‑1 (MQ=255)                                                                   gAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGt                                                                    <  2:422387/140‑1 (MQ=255)                                                                      ccAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAg                                                                 <  1:907270/140‑1 (MQ=255)                                                                       cAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGt                                                                <  1:287796/140‑1 (MQ=255)                                                                               ttCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACa                                                                         >  2:928303/1‑123 (MQ=255)                                                                               ttCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACa                                                                         <  1:928303/123‑1 (MQ=255)                                                                               ttCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGc                                                        >  2:553374/1‑140 (MQ=255)                                                                                       gCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGttt                                                >  1:719177/1‑140 (MQ=255)                                                                                         gTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCg                                              <  1:619070/140‑1 (MQ=255)                                                                                          ttCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCg                                              <  1:240544/139‑1 (MQ=255)                                                                                          ttCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCg                                              >  2:240544/1‑139 (MQ=255)                                                                                          ttCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGc                                             >  1:474945/1‑140 (MQ=255)                                                                                          ttCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGc                                             >  2:493335/1‑140 (MQ=255)                                                                                          ttCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGc                                             >  2:276898/1‑140 (MQ=255)                                                                                                ggCGTGATACATCATGGTCATGCCCGGCGGTACACG‑TGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCg                                              <  1:200978/132‑1 (MQ=255)                                                                                                ggCGTGATACATCATGGTCATGCCCGGCGGTACACG‑TGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCg                                              >  2:200978/1‑132 (MQ=255)                                                                                                     gATACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACa                                  >  2:68979/1‑140 (MQ=255)                                                                                                       tACATCATGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAAt                                >  2:360883/1‑140 (MQ=255)                                                                                                              tGGTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCa                         <  1:827106/140‑1 (MQ=255)                                                                                                               ggTCATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCAc                        >  1:368566/1‑140 (MQ=255)                                                                                                                  cATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTc                           <  2:1086186/134‑1 (MQ=255)                                                                                                                  cATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTc                           <  2:233051/134‑1 (MQ=255)                                                                                                                  cATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTc                           >  1:233051/1‑134 (MQ=255)                                                                                                                  cATGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTc                           >  1:1086186/1‑134 (MQ=255)                                                                                                                    tGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTc                           >  2:1053957/1‑132 (MQ=255)                                                                                                                    tGCCCGGCGGTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTc                           <  1:1053957/132‑1 (MQ=255)                                                                                                                          gcggTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGc             >  2:428355/1‑140 (MQ=255)                                                                                                                           cggTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCg            >  2:596408/1‑140 (MQ=255)                                                                                                                             gTACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTc          >  1:311363/1‑140 (MQ=255)                                                                                                                              tACACGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCa         <  2:1127333/140‑1 (MQ=255)                                                                                                                                cacGTTGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGc       <  1:834023/140‑1 (MQ=255)                                                                                                                                     tGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAg  >  2:263594/1‑140 (MQ=255)                                                                                                                                     tGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAg  <  2:505669/140‑1 (MQ=255)                                                                                                                                     tGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAg  >  1:505669/1‑140 (MQ=255)                                                                                                                                     tGGTTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAg  >  2:996618/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         TGCGTTGGTTTCGGGCAAACGCGGGTAACCGAGTTATGAATGCCGCCGCGCATGCCAGTTACTTCCGAACCAGGAATATTCATAATGCGTTCCTGGGCGTGATACATCATGGTCATGCCCGGCGGTACACGTTGGCTGACCACCGCGCGGGCAGTCAGCGCGCCGTTGGCGTTGAATACTTCCACCCAGTCGTTATCGACAATGGTCAGTTCTCGGGCATCTGTTTCGCTGATCCAGACAATCGGTCCACCGCGAGAGAGCGTCAGCATTAG  >  NC_000913/1539004‑1539275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑