| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,541,351 | C→T | E414K (GAA→AAA) | narZ ← | nitrate reductase Z subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,541,351 | 0 | C | T | 97.8% | 149.7 / ‑2.9 | 45 | E414K (GAA→AAA) | narZ | nitrate reductase Z subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (17/27); minor base G (1/0); total (18/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGT > NC_000913/1541215‑1541482 | tttttAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGt < 2:732667/140‑1 (MQ=255) ggCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTAcc < 2:592650/140‑1 (MQ=255) ggCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTAcc > 2:579660/1‑140 (MQ=255) ggCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTAcc > 1:560124/1‑140 (MQ=255) ggCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCa < 2:794529/140‑1 (MQ=255) cACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATgg < 2:464461/140‑1 (MQ=255) cAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGgatt < 2:370875/140‑1 (MQ=255) ccGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGgattccaga < 2:251748/140‑1 (MQ=255) tgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAtt > 1:377157/1‑139 (MQ=255) tgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAtt < 2:377157/139‑1 (MQ=255) tgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAt < 2:335264/138‑1 (MQ=255) tgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAt > 1:335264/1‑138 (MQ=255) tttACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGTGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAC‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGcc < 2:1097486/140‑1 (MQ=255) tttACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGcc < 2:242286/140‑1 (MQ=255) tttACCCTGCGAAAATGGCGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGGTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTTCCGGCGGCAATGGATTCCAGATTCCATTTGcc < 2:404082/140‑1 (MQ=255) ttACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCt > 2:458539/1‑140 (MQ=255) aaTGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAg < 2:632629/140‑1 (MQ=255) aTGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGc < 2:189151/140‑1 (MQ=255) ggATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGaa < 2:808313/140‑1 (MQ=255) gATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGaaa > 1:736275/1‑140 (MQ=255) aaGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCgg < 1:458539/140‑1 (MQ=255) ggggAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCg < 2:743866/130‑1 (MQ=255) ggggAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCg > 1:743866/1‑130 (MQ=255) gggAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTAc > 2:1128294/1‑140 (MQ=255) gAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt < 2:79071/136‑1 (MQ=255) gAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt > 1:79071/1‑136 (MQ=255) aaGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCaa < 2:560124/140‑1 (MQ=255) ggCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAAtt > 2:848083/1‑140 (MQ=255) gCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTc > 2:918382/1‑140 (MQ=255) gCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTc < 2:464921/140‑1 (MQ=255) cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt < 1:918382/140‑1 (MQ=255) cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt < 1:1128294/140‑1 (MQ=255) gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg < 1:848083/140‑1 (MQ=255) ccTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGc < 1:700384/140‑1 (MQ=255) aCAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTAtt < 1:80445/140‑1 (MQ=255) cGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAAc > 2:370746/1‑140 (MQ=255) cGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTAtt < 2:930228/136‑1 (MQ=255) cGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTAtt > 1:930228/1‑136 (MQ=255) ccGAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGtttt > 1:959731/1‑140 (MQ=255) gAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTa < 2:165658/131‑1 (MQ=255) gAGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTa > 1:165658/1‑131 (MQ=255) aGCAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCa > 2:616728/1‑140 (MQ=255) cAGGGTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACt > 2:224838/1‑140 (MQ=255) ggTTAACGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCg < 1:289232/140‑1 (MQ=255) aCGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttg > 1:46833/1‑140 (MQ=255) cGACAA‑TTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttgt < 1:616728/140‑1 (MQ=255) | TTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAA‑TTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGT > NC_000913/1541215‑1541482 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |