Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	213,589	A→G	E420G (GAG→GGG)	tilS →	tRNA(Ile)‑lysidine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	213,589	0	A	G	100.0%	187.3 / NA	52	E420G (GAG→GGG)	tilS	tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/28);  total (24/28)

CGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCGATTTCCGGGTGGCTAAAGC  >  NC_000913/213453‑213724                                                                                                                                         |                                                                                                                                        cGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGaa                                                                                                                                      <  1:1025924/140‑1 (MQ=255)      gCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCg                                                                                                                                 <  2:561835/140‑1 (MQ=255)       cTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGt                                                                                                                                <  1:669033/140‑1 (MQ=255)               aaTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTg                                                                                                                        >  2:959867/1‑140 (MQ=255)                aTCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGt                                                                                                                       >  1:835644/1‑140 (MQ=255)                       aaGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGGATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAg                                                                                                                >  1:639539/1‑140 (MQ=255)                         gagCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGaa                                                                                                              <  2:91848/140‑1 (MQ=255)                          agCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGaaa                                                                                                             <  2:653826/140‑1 (MQ=255)                            cTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAAc                                                                                                           >  2:269131/1‑140 (MQ=255)                               ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                                        >  1:1032379/1‑140 (MQ=255)                               ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                                        <  2:835644/140‑1 (MQ=255)                               ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                                        <  2:123797/140‑1 (MQ=255)                               ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                                        >  1:123797/1‑140 (MQ=255)                               ggCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCt                                                                                                        <  1:269131/140‑1 (MQ=255)                                                     acacCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATaa                                                                                  >  2:431278/1‑140 (MQ=255)                                                           cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                                            >  1:540735/1‑140 (MQ=255)                                                           cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                                            >  1:1159977/1‑140 (MQ=255)                                                           cGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                                            >  1:400429/1‑140 (MQ=255)                                                              cACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                                            >  1:613534/1‑137 (MQ=255)                                                              cACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCa                                                                            <  2:613534/137‑1 (MQ=255)                                                                ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATa                                                                                   <  2:1095655/128‑1 (MQ=255)                                                                ctgctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATa                                                                                   >  1:1095655/1‑128 (MQ=255)                                                                   ctgTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGt                                                                    <  2:434641/140‑1 (MQ=255)                                                                     gTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                                                                 >  1:1089870/1‑125 (MQ=255)                                                                     gTTTTATGGCGAAACGCTGATTGCGGCGACAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAg                                                                                 <  2:1089870/125‑1 (MQ=255)                                                                           tGGCGAAACGCTGATGGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCg                                                            >  2:204496/1‑140 (MQ=255)                                                                            ggCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCgg                                                           <  1:959867/140‑1 (MQ=255)                                                                              cGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCg                                                         <  2:949771/140‑1 (MQ=255)                                                                                aaaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGc                                                          >  1:491766/1‑137 (MQ=255)                                                                                aaaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGc                                                          <  2:491766/137‑1 (MQ=255)                                                                                aaaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaa                                                       >  2:885538/1‑140 (MQ=255)                                                                                aaaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaa                                                       >  1:618422/1‑140 (MQ=255)                                                                                aaaCGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGaa                                                       >  1:582545/1‑140 (MQ=255)                                                                                          tGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAg                                             >  1:111921/1‑140 (MQ=255)                                                                                           gcggcgGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAgg                                            <  2:290437/140‑1 (MQ=255)                                                                                                ggcAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                                       <  1:546434/140‑1 (MQ=255)                                                                                                  cAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                                     <  2:850418/140‑1 (MQ=255)                                                                                                  cAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCt                                     >  1:721529/1‑140 (MQ=255)                                                                                                     gggTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTac                                  <  2:793011/140‑1 (MQ=255)                                                                                                     gggTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTac                                  <  2:833038/140‑1 (MQ=255)                                                                                                           ttGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                                       <  1:817034/129‑1 (MQ=255)                                                                                                           ttGTGACGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAATGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCg                                       >  2:817034/1‑129 (MQ=255)                                                                                                                aCGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAc                       <  2:400429/140‑1 (MQ=255)                                                                                                                aCGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAc                       <  2:618422/140‑1 (MQ=255)                                                                                                                aCGCAAGAAGGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTAc                       <  1:885538/140‑1 (MQ=255)                                                                                                                     agaagGTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCGAtt                  >  2:563462/1‑140 (MQ=255)                                                                                                                         ggTGTGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCGATTTCCg              <  1:971059/140‑1 (MQ=255)                                                                                                                          gtgtGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCGATTTCCgg             <  2:540735/140‑1 (MQ=255)                                                                                                                          gtgtGGCTGAAGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCGATTTCCgg             <  1:563462/140‑1 (MQ=255)                                                                                                                                   aaGGTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCGATTTCCGGGTGGCTaaa    <  2:1159977/140‑1 (MQ=255)                                                                                                                                     ggTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCGATTTCCGGGTGGCTAAAGc  >  1:504372/1‑140 (MQ=255)                                                                                                                                     ggTGGGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCGATTTCCGGGTGGCTAAAGc  >  1:1044765/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CGTAAGCTAAAGAAAATCTGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGCGAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAGAATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAAGTGAAAGCCGGATAAGACGCATCAAACGTCGCATCCGGCGAAAGTCAATCAGGATTCGCTTACCACCACCGTACCGATTTCCGGGTGGCTAAAGC  >  NC_000913/213453‑213724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑