Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,640	G→A	A362T (GCC→ACC)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,640	0	G	A	100.0%	112.2 / NA	34	A362T (GCC→ACC)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/14);  total (20/14)

TTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGG  >  NC_000913/1680505‑1680770                                                                                                                                        |                                                                                                                                   ttCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTc                                                                                                                                >  2:606234/1‑140 (MQ=255) ttCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTc                                                                                                                                >  1:772800/1‑140 (MQ=255)                gcgcgcCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGt                                                                                                                 <  2:647151/140‑1 (MQ=255)                                aaGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTc                                                                                                 >  2:84513/1‑140 (MQ=255)                                aaGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTc                                                                                                 >  2:757522/1‑140 (MQ=255)                                         cATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTc                                                                                        <  2:772800/140‑1 (MQ=255)                                            ccGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTg                                                                                     >  2:510063/1‑140 (MQ=255)                                                            gACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAAccc                                                                     >  2:166755/1‑140 (MQ=255)                                                            gACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAAccc                                                                     <  1:606234/140‑1 (MQ=255)                                                             aCCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCg                                                                    >  1:738367/1‑140 (MQ=255)                                                                aaCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTcc                                                                 >  2:312724/1‑140 (MQ=255)                                                                 aCATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCa                                                                >  1:961205/1‑140 (MQ=255)                                                                   aTCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACt                                                              >  2:436881/1‑140 (MQ=255)                                                                       gCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCAt                                                          <  1:312724/140‑1 (MQ=255)                                                                         gTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATaa                                                        <  1:166755/140‑1 (MQ=255)                                                                            cAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGc                                                     >  2:60566/1‑140 (MQ=255)                                                                            cAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGc                                                     >  2:1127765/1‑140 (MQ=255)                                                                               aTTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGt                                                  <  1:60566/140‑1 (MQ=255)                                                                                    tCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGgtg                                             >  1:220188/1‑140 (MQ=255)                                                                                     cTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGgtgt                                            <  1:436881/140‑1 (MQ=255)                                                                                         tACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGt                                        >  1:590242/1‑140 (MQ=255)                                                                                           cTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGc                                      >  2:1157424/1‑140 (MQ=255)                                                                                             cATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCt                                    <  1:600370/140‑1 (MQ=255)                                                                                             cATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCt                                    <  1:1127765/140‑1 (MQ=255)                                                                                                    cTGACCGGTTCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTa                             <  1:930613/140‑1 (MQ=255)                                                                                                             tCCGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATg                    >  1:570857/1‑140 (MQ=255)                                                                                                               cGATTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGt                  <  1:84513/140‑1 (MQ=255)                                                                                                                 aTTACAACACGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTa                >  2:886774/1‑140 (MQ=255)                                                                                                                      aacaCGTTGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTa           <  1:723780/140‑1 (MQ=255)                                                                                                                             tGCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCctct    >  2:842771/1‑140 (MQ=255)                                                                                                                              gCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTg   <  1:842771/140‑1 (MQ=255)                                                                                                                              gCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTg   >  2:399778/1‑140 (MQ=255)                                                                                                                              gCTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTg   <  2:961205/140‑1 (MQ=255)                                                                                                                               cTGACCATCACCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCACAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTgg  >  2:835025/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   TTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCCGCCTCACTGTGGTTGACCAACATCTGCGTGCAAATTTGTCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTCGGTGCCTGCATTTATGGCTTATGGTTATTGTACGCCTCTGG  >  NC_000913/1680505‑1680770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑