Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,784,530	T→C	R518R (CGT→CGC)	fadK →	short chain acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,784,530	0	T	C	100.0%	193.9 / NA	53	R518R (CGT→CGC)	fadK	short chain acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/31);  total (22/31)

CAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAGGATGTCTGTGA  >  NC_000913/1784393‑1784664                                                                                                                                          |                                                                                                                                       cagcaTCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCaa                                                                                                                                      >  1:301103/1‑140 (MQ=255) cagcaTCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCaa                                                                                                                                      >  1:990341/1‑140 (MQ=255)      tCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACggg                                                                                                                                 >  2:1116878/1‑140 (MQ=255)          aaaaTTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGc                                                                                                                             <  2:301103/140‑1 (MQ=255)          aaaaTTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGc                                                                                                                             <  1:784415/140‑1 (MQ=255)           aaaTTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCa                                                                                                                            <  2:966245/140‑1 (MQ=255)           aaaTTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCa                                                                                                                            <  2:102452/140‑1 (MQ=255)             aTTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCaaa                                                                                                                          <  2:325440/140‑1 (MQ=255)              ttCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCaaaa                                                                                                                         <  1:1116878/140‑1 (MQ=255)              ttCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCaaaa                                                                                                                         <  2:713468/140‑1 (MQ=255)                 aCGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAaaatat                                                                                                                      >  1:1082432/1‑140 (MQ=255)                  cGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCa                                                                                                                            >  1:634603/1‑133 (MQ=255)                  cGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCa                                                                                                                            <  2:634603/133‑1 (MQ=255)                            gtgGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCTGCAAACGGGTCGCAAAATATAAATATCCTGa                                                                                                           <  2:885509/140‑1 (MQ=255)                            gtgGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGa                                                                                                           <  2:73186/140‑1 (MQ=255)                             tgGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGaa                                                                                                          <  2:1082432/140‑1 (MQ=255)                                 tGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACata                                                                                                      >  2:589528/1‑140 (MQ=255)                                 tGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACata                                                                                                      >  2:416502/1‑140 (MQ=255)                                  gCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACatat                                                                                                     >  2:992216/1‑140 (MQ=255)                                  gCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACatat                                                                                                     >  2:518858/1‑140 (MQ=255)                                   cAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGaa                                                                                                          >  2:254586/1‑134 (MQ=255)                                   cAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGaa                                                                                                          <  1:254586/134‑1 (MQ=255)                                           gATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAAt                                                                                            <  2:943734/140‑1 (MQ=255)                                                  gTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGaaaaa                                                                                     <  1:542147/140‑1 (MQ=255)                                                           aaCGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGa                                                                            >  2:689666/1‑140 (MQ=255)                                                            aCGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGaa                                                                           <  1:992216/140‑1 (MQ=255)                                                            aCGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGaa                                                                           <  1:961607/140‑1 (MQ=255)                                                             cGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAAc                                                                          <  2:662376/140‑1 (MQ=255)                                                                  aTGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGC‑TTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTc                                                                    >  1:344879/1‑140 (MQ=255)                                                                  aTGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGC‑TTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTc                                                                    <  2:344879/140‑1 (MQ=255)                                                                   tGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTc                                                                    >  1:576635/1‑140 (MQ=255)                                                                   tGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTc                                                                    <  1:87650/140‑1 (MQ=255)                                                                          aTGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTaaa                                                             >  1:448889/1‑140 (MQ=255)                                                                          aTGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTaaa                                                             >  1:703653/1‑140 (MQ=255)                                                                                  cTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACaaaa                                                     >  2:1150242/1‑140 (MQ=255)                                                                                      aaGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGttt                                                 <  1:596880/140‑1 (MQ=255)                                                                                       aGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGtttt                                                >  2:375252/1‑140 (MQ=255)                                                                                               atcatTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAg                                         >  1:543568/1‑139 (MQ=255)                                                                                               atcatTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAg                                         <  2:543568/139‑1 (MQ=255)                                                                                               atcatTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGa                                        <  1:589528/140‑1 (MQ=255)                                                                                                      attATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGata                                 <  2:608376/140‑1 (MQ=255)                                                                                                      attATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGata                                 <  2:245175/140‑1 (MQ=255)                                                                                                             cTGGAAGAGGTAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATgcg                          >  1:625385/1‑140 (MQ=255)                                                                                                                       tAGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACg                <  2:576635/140‑1 (MQ=255)                                                                                                                        aGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTaa                  <  2:176355/137‑1 (MQ=255)                                                                                                                        aGTGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTaa                  >  1:176355/1‑137 (MQ=255)                                                                                                                          tGGCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAg             >  1:948171/1‑140 (MQ=255)                                                                                                                            gCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAGGa           <  1:689666/140‑1 (MQ=255)                                                                                                                            gCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAGGa           <  2:948171/140‑1 (MQ=255)                                                                                                                            gCTTTTTTTAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAGGa           <  2:307157/140‑1 (MQ=255)                                                                                                                              tttttttAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAGGAt          <  1:215954/139‑1 (MQ=255)                                                                                                                              tttttttAGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAGGAt          >  2:215954/1‑139 (MQ=255)                                                                                                                                     aGCCGCAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAGGATGTCTGTGa  <  1:1150242/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATATCGTGGTAATCGAAAAACTACCGCGAACTACCTCAGGTAAAATACAAAAGTTTTTGTTAAGAAAAGATATTATGCGGCGTTTAACGCAGGATGTCTGTGA  >  NC_000913/1784393‑1784664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑