Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	225,800	A→G	noncoding (42/2904 nt)	rrlH →	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	225,800	0	A	G	100.0%	68.0 / NA	26	noncoding (42/2904 nt)	rrlH	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/9);  total (17/9)

TGAAAATTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG  >  NC_000913/225664‑225875                                                                                                                                         |                                                                            tGAAAATTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCggag                                                                          <  2:317157/140‑1 (MQ=37)           aaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGTAGTCGGAGGCGATGAAgg                                                                <  1:1127694/140‑1 (MQ=255)                  aaCAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCt                                                         >  2:990262/1‑140 (MQ=255)                            gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGAt                                               >  2:259176/1‑140 (MQ=37)                            gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGAt                                               >  1:893721/1‑140 (MQ=37)                               gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAg                                            <  2:831343/140‑1 (MQ=21)                               gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAg                                            <  2:587277/140‑1 (MQ=21)                                  cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGt                                         <  2:80617/140‑1 (MQ=21)                                     gAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCgg                                      <  1:990262/140‑1 (MQ=21)                                          tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATaa                                             >  1:686136/1‑128 (MQ=37)                                          tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATaa                                             <  2:686136/128‑1 (MQ=25)                                          tctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAgg                                 >  1:373939/1‑140 (MQ=37)                                                   ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAg                                  <  1:1158959/130‑1 (MQ=25)                                                   ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAg                                  >  2:1158959/1‑130 (MQ=37)                                                   ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGaa                        <  2:893721/140‑1 (MQ=21)                                                   ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGaa                        >  2:165976/1‑140 (MQ=37)                                                                    gATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGAt       >  1:427512/1‑140 (MQ=18)                                                                    gATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGAt       >  1:287375/1‑140 (MQ=18)                                                                    gATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGAt       >  1:1016396/1‑140 (MQ=18)                                                                    gATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGAt       >  1:857803/1‑140 (MQ=18)                                                                     aTCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGAtt      >  1:570902/1‑140 (MQ=18)                                                                      tCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGc          >  2:1099744/1‑135 (MQ=18)                                                                      tCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGc          <  1:1099744/135‑1 (MQ=17)                                                                        gCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTct   >  2:1152491/1‑139 (MQ=14)                                                                         cAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGATCCGTTATAACCGGCGATTTCCg  >  2:976720/1‑140 (MQ=11)                                                                         cAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCg  >  1:685477/1‑140 (MQ=11)                                                                         cAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCGGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCg  >  1:264743/1‑140 (MQ=11)                                                                                                                                         |                                                                            TGAAAATTGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG  >  NC_000913/225664‑225875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑