Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,347,256	A→G	intergenic (+106/‑128)	nrdA → / → nrdB	ribonucleoside‑diphosphate reductase 1 subunit alpha/ribonucleoside‑diphosphate reductase 1 subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,347,256	0	A	G	100.0%	24.9 / NA	10	intergenic (+106/‑128)	nrdA/nrdB	ribonucleoside‑diphosphate reductase 1 subunit alpha/ribonucleoside‑diphosphate reductase 1 subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/4);  total (6/4)

GGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCA  >  NC_000913/2347243‑2347389              |                                                                                                                                      agatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAAc                   <  1:1155221/126‑1 (MQ=18) agatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAAc                   >  2:1155221/4‑129 (MQ=17) agatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTc        >  2:409666/4‑140 (MQ=18) agatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTc        >  2:579694/4‑140 (MQ=18)  gatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCa       <  1:297930/138‑1 (MQ=255)   atGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGaca             <  1:404027/132‑1 (MQ=255)   atGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGaca             >  2:404027/2‑133 (MQ=18)     gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATgg    >  1:1099376/1‑140 (MQ=255)     gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATgg    >  1:449483/1‑140 (MQ=255)     gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATgg    <  2:364504/140‑1 (MQ=255)       cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCa  <  1:9608/140‑1 (MQ=255)              |                                                                                                                                      GGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCA  >  NC_000913/2347243‑2347389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑