Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,347,261	G→A	intergenic (+111/‑123)	nrdA → / → nrdB	ribonucleoside‑diphosphate reductase 1 subunit alpha/ribonucleoside‑diphosphate reductase 1 subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,347,261	0	G	A	100.0%	38.2 / NA	14	intergenic (+111/‑123)	nrdA/nrdB	ribonucleoside‑diphosphate reductase 1 subunit alpha/ribonucleoside‑diphosphate reductase 1 subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/4);  total (10/4)

GGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCATATACCACC  >  NC_000913/2347243‑2347398                   |                                                                                                                                          agatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAAc                            <  1:1155221/126‑1 (MQ=18) agatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAAc                            >  2:1155221/4‑129 (MQ=17) agatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTc                 >  2:409666/4‑140 (MQ=18) agatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTc                 >  2:579694/4‑140 (MQ=18)  gatGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCa                <  1:297930/138‑1 (MQ=255)   atGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGaca                      <  1:404027/132‑1 (MQ=255)   atGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGaca                      >  2:404027/2‑133 (MQ=18)     gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATgg             >  1:1099376/1‑140 (MQ=255)     gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATgg             >  1:449483/1‑140 (MQ=255)     gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATgg             <  2:364504/140‑1 (MQ=255)       cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCa           <  1:9608/140‑1 (MQ=255)             cggCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCATATacc     >  1:963760/3‑140 (MQ=255)              ggCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCATATacca    >  2:379073/2‑140 (MQ=255)               gCGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCATATaccac   >  2:890305/1‑140 (MQ=255)                cGTAAACGCCTTATCCGGCCTACGGCTCGG‑TTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCATATaccacc  >  2:994543/1‑140 (MQ=255)                   |                                                                                                                                          GGGTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACGGCTCGGATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCACAGGATGCGGCGTAAAATGCCTTATCCGGCATTAAACTCCCAACAGGACACACTCATGGCATATACCACC  >  NC_000913/2347243‑2347398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑