Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,377,690	C→T	A524T (GCC→ACC)	menD ←	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,377,690	0	C	T	100.0%	120.0 / NA	36	A524T (GCC→ACC)	menD	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/13);  total (23/13)

GTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTT  >  NC_000913/2377559‑2377823                                                                                                                                    |                                                                                                                                      gTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCaa                                                                                                                               >  1:1011523/1‑140 (MQ=255)     tCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGc                                                                                                                           <  1:86774/140‑1 (MQ=255)                                 aTAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGa                                                                                               >  2:455467/1‑140 (MQ=255)                                  tAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGAc                                                                                              <  2:1011523/140‑1 (MQ=255)                                       ggCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACgatga                                                                                         >  1:912793/1‑140 (MQ=255)                                        gCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACgatgat                                                                                        >  1:360764/1‑140 (MQ=255)                                         cTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGa                                                                                               >  1:33714/1‑132 (MQ=255)                                         cTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGa                                                                                               <  2:33714/132‑1 (MQ=255)                                         cTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGata                                                                                       >  1:1024885/1‑140 (MQ=255)                                         cTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGata                                                                                       >  2:595405/1‑140 (MQ=255)                                         cTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGata                                                                                       >  2:471716/1‑140 (MQ=255)                                         cTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGata                                                                                       >  2:354101/1‑140 (MQ=255)                                           tACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATAtt                                                                                     >  2:202016/1‑140 (MQ=255)                                            aCCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATAttt                                                                                    <  1:455467/140‑1 (MQ=255)                                                gcgcCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGc                                                                                <  2:467326/140‑1 (MQ=255)                                                  gcCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTc                                                                              >  1:226080/1‑140 (MQ=255)                                                      gAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAAc                                                                          >  2:445681/1‑140 (MQ=255)                                                                gAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCgcggcgg                                                                >  2:835632/1‑140 (MQ=255)                                                                  gCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCgcggcggcg                                                              <  2:912793/140‑1 (MQ=255)                                                                   cGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGt                                                             >  1:501895/1‑140 (MQ=255)                                                                    gTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTg                                                            <  2:226080/140‑1 (MQ=255)                                                                                tCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGAc                                                >  1:280308/1‑140 (MQ=255)                                                                                           aaCCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCa                                     <  2:280308/140‑1 (MQ=255)                                                                                           aaCCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCa                                     <  1:48553/140‑1 (MQ=255)                                                                                           aaCCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCa                                     <  2:360764/140‑1 (MQ=255)                                                                                            accaccATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCAt                                    <  1:595405/140‑1 (MQ=255)                                                                                                ccaTTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAATTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGa                                >  2:1066575/1‑140 (MQ=255)                                                                                                          cACCGTGGTGGTTGGCGTGCGCCAGGTGTTGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAacgct                      >  2:712582/1‑140 (MQ=255)                                                                                                             cGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAacgctcac                   >  2:484519/1‑140 (MQ=255)                                                                                                             cGTGGTGGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAacgctcac                   >  1:765449/1‑140 (MQ=255)                                                                                                              gtggtgGTTGGCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAacgctcacg                  >  2:55749/1‑140 (MQ=255)                                                                                                                       ggCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTg         >  1:998118/1‑140 (MQ=255)                                                                                                                       ggCGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTg         <  1:55749/140‑1 (MQ=255)                                                                                                                         cGTGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTgcg       <  1:835632/140‑1 (MQ=255)                                                                                                                           tGCGCCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTgcggc     >  1:322592/1‑140 (MQ=255)                                                                                                                              gcCAGGTGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGtt  >  1:711409/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      GTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCTTACCTGCGCCAGAAGTTGCTGGAGCGTTTGCGCACCATCGGTGTCGTTAACCACCATTTCAATCACCGTGGTGGTTGGCGTGCGCCAGGCGTCGGCAAATGCCGTTTCAAGTTCCTGCCAGTTTTGCGGACGATGATATTTCAGCTCGAACATCGCGGCGGCGTGCTCAAAATGGACGTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTT  >  NC_000913/2377559‑2377823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑