Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,426,793	T→C	intergenic (‑5/+216)	hisJ ← / ← argT	histidine ABC transporter periplasmic binding protein/lysine/arginine/ornithine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,426,793	0	T	C	100.0%	138.5 / NA	38	intergenic (‑5/+216)	hisJ/argT	histidine ABC transporter periplasmic binding protein/lysine/arginine/ornithine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/21);  total (17/21)

GTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGT  >  NC_000913/2426656‑2426922                                                                                                                                          |                                                                                                                                  gtgAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGccc                                                                                                                                 >  1:422834/1‑140 (MQ=255)    aaTTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCa                                                                                                                              <  2:281846/140‑1 (MQ=255)      ttttttGATTCCAATGGCGCATAGGTCGGGTCGGTGCCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCGCCAGTTTTTTCATAATGCCCTCAAc                                                                                                                            <  2:376716/140‑1 (MQ=255)         tttGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGa                                                                                                                         <  2:554180/140‑1 (MQ=255)          ttGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGAc                                                                                                                        <  2:997634/140‑1 (MQ=255)                 aaaTGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCttt                                                                                                                 <  2:791273/140‑1 (MQ=255)                        gcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAgg                                                                                                          <  1:477776/140‑1 (MQ=255)                           tAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTta                                                                                                              <  1:559608/133‑1 (MQ=255)                           tAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTta                                                                                                              >  2:559608/1‑133 (MQ=255)                                cgggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATAttt                                                                                                  >  2:88955/1‑140 (MQ=255)                                   gtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACa                                                                                               >  2:563190/1‑140 (MQ=255)                                    tcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                              <  1:212670/140‑1 (MQ=255)                                      ggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGt                                                                                            <  1:344534/140‑1 (MQ=255)                                      ggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGt                                                                                            <  1:88955/140‑1 (MQ=255)                                             aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                              <  2:883459/131‑1 (MQ=255)                                             aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                              >  1:883459/1‑131 (MQ=255)                                             aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTac                                                                                     >  1:850016/1‑140 (MQ=255)                                                  gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTa                                                                                >  2:570509/1‑140 (MQ=255)                                                     gTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCg                                                                             <  1:915862/140‑1 (MQ=255)                                                        ttGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGc                                                                          >  1:1056369/1‑140 (MQ=255)                                                         tGCGGAATCGCAGCAAACGCCACAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGcc                                                                         <  1:686858/140‑1 (MQ=255)                                                          gCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCa                                                                        <  1:563190/140‑1 (MQ=255)                                                                           gccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAg                                                       <  2:1056369/140‑1 (MQ=255)                                                                                aGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCaaa                                                  <  2:167951/140‑1 (MQ=255)                                                                                aGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCaaa                                                  <  1:985495/140‑1 (MQ=255)                                                                                aGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCaaa                                                  >  2:985495/1‑140 (MQ=255)                                                                                        tGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCg                                          <  1:927206/140‑1 (MQ=255)                                                                                                    gAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCg                                          <  1:961291/128‑1 (MQ=255)                                                                                                    gAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCg                                          >  2:961291/1‑128 (MQ=255)                                                                                                          gagagagCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtattat                        >  2:316639/1‑140 (MQ=255)                                                                                                          gagagagCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtattat                        >  2:896308/1‑140 (MQ=255)                                                                                                               agCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTc                   >  2:247381/1‑140 (MQ=255)                                                                                                               agCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTc                   >  2:1002226/1‑140 (MQ=255)                                                                                                               agCGATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTc                   >  1:939792/1‑140 (MQ=255)                                                                                                               agCGATAGCACCAGTTTTTTCATAATGCCCTCAACTAACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGATCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTc                   >  2:1090317/1‑140 (MQ=255)                                                                                                                  gATAGCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCAtt                >  2:608851/1‑140 (MQ=255)                                                                                                                      gCACCAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTaaaa            >  2:875814/1‑140 (MQ=255)                                                                                                                         ccAGTTTTTTCATAATGCCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATg         <  1:998937/140‑1 (MQ=255)                                                                                                                                tttCATAATGCCCTCAACTAACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGt  <  1:1090317/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  GTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTTTTTTCATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAAATGCTTAAGT  >  NC_000913/2426656‑2426922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑