Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,443,240	G→A	A493T (GCA→ACA)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,443,240	0	G	A	100.0%	226.0 / NA	65	A493T (GCA→ACA)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/34);  total (31/34)

AGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGC  >  NC_000913/2443107‑2443369                                                                                                                                      |                                                                                                                                  agaTCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGc                                                                                                                             <  2:118219/140‑1 (MQ=255)     cAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGaa                                                                                                                         >  1:884053/1‑140 (MQ=255)       gcaagcaaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGc                                                                                                                       <  1:300743/140‑1 (MQ=255)          agcaaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAgg                                                                                                                    >  1:297576/1‑140 (MQ=255)            caaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtg                                                                                                                  >  1:1013463/1‑140 (MQ=255)            caaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtg                                                                                                                  <  2:218327/140‑1 (MQ=255)            caaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtg                                                                                                                  <  2:297576/140‑1 (MQ=255)            caaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtg                                                                                                                  <  1:270329/140‑1 (MQ=255)            caaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtg                                                                                                                  <  2:1013463/140‑1 (MQ=255)            caaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtg                                                                                                                  <  2:884053/140‑1 (MQ=255)            caaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtg                                                                                                                  <  2:888168/140‑1 (MQ=255)             aaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtgc                                                                                                                 >  1:1153485/1‑140 (MQ=255)             aaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtgc                                                                                                                 >  2:588026/1‑140 (MQ=255)             aaCACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtgc                                                                                                                 >  1:670093/1‑140 (MQ=255)               cacacaGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtgctg                                                                                                               <  2:253721/140‑1 (MQ=255)                acacaGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtgctgt                                                                                                              <  2:670093/140‑1 (MQ=255)                  acaGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGgtgctgtgc                                                                                                            >  2:585307/1‑140 (MQ=255)                     gCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTAt                                                                                                         >  2:480442/1‑140 (MQ=255)                     gCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTAt                                                                                                         <  1:935221/140‑1 (MQ=255)                      cGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATg                                                                                                        >  1:807449/1‑140 (MQ=255)                      cGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATg                                                                                                        >  2:463416/1‑140 (MQ=255)                         aGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACg                                                                                                     >  1:899408/1‑140 (MQ=255)                           tGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGt                                                                                                   >  2:1128004/1‑140 (MQ=255)                                cTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTAtct                                                                                              >  2:1157409/1‑140 (MQ=255)                                       aCGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCca                                                                                       <  2:374894/140‑1 (MQ=255)                                       aCGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCca                                                                                       >  1:1169333/1‑140 (MQ=255)                                       aCGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCca                                                                                       <  1:1124365/140‑1 (MQ=255)                                        cGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCcac                                                                                      >  1:696797/1‑140 (MQ=255)                                        cGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCcac                                                                                      >  2:275964/1‑140 (MQ=255)                                        cGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCcac                                                                                      >  2:855786/1‑140 (MQ=255)                                                    cAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGa                                                                          <  2:735103/140‑1 (MQ=255)                                                      gCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAAt                                                                        <  1:308512/140‑1 (MQ=255)                                                      gCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAAt                                                                        <  1:972209/140‑1 (MQ=255)                                                         gATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCaa                                                                     <  2:1122835/140‑1 (MQ=255)                                                          aTTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAc                                                                    <  2:1169333/140‑1 (MQ=255)                                                          aTTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAc                                                                    <  2:942732/140‑1 (MQ=255)                                                               gCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAAcatcat                                                               >  1:1123846/1‑140 (MQ=255)                                                                     cGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTAtt                                                         >  2:748055/1‑140 (MQ=255)                                                                      gTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTg                                                        <  1:108863/140‑1 (MQ=255)                                                                       tCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTgg                                                       <  1:1157409/140‑1 (MQ=255)                                                                       tCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTgg                                                       <  1:424115/140‑1 (MQ=255)                                                                         gACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTg                                                     <  1:275964/140‑1 (MQ=255)                                                                          aCTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGc                                                    <  2:115226/140‑1 (MQ=255)                                                                          aCTCACCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGc                                                    >  1:1101099/1‑140 (MQ=255)                                                                                ccGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTa                                              >  1:1154380/1‑140 (MQ=255)                                                                                        ttCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATcgcg                                      >  1:1090849/1‑140 (MQ=255)                                                                                              tGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCg                                >  2:457359/1‑140 (MQ=255)                                                                                                ccGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGaa                              >  1:445017/1‑140 (MQ=255)                                                                                                   ggCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGAt                           <  1:386592/140‑1 (MQ=255)                                                                                                   ggCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGAt                           <  1:1132770/140‑1 (MQ=255)                                                                                                   ggCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGAt                           <  1:480442/140‑1 (MQ=255)                                                                                                   ggCAGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGAt                           <  2:1090849/140‑1 (MQ=255)                                                                                                      aGGTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACg                        <  1:1132897/140‑1 (MQ=255)                                                                                                       ggTCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACgg                       >  1:783296/1‑140 (MQ=255)                                                                                                         tCAGCCATATTCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACAACATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCg                     <  2:807449/140‑1 (MQ=255)                                                                                                                  ttCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAg            >  2:427691/1‑140 (MQ=255)                                                                                                                  ttCCGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAg            >  1:632641/1‑140 (MQ=255)                                                                                                                    ccGACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGa          <  2:899408/140‑1 (MQ=255)                                                                                                                      gACAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACg        <  2:1154380/140‑1 (MQ=255)                                                                                                                        cAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGAt      <  2:696797/140‑1 (MQ=255)                                                                                                                        cAACGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGAt      <  2:870983/140‑1 (MQ=255)                                                                                                                          aCGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGCGAGGACGATca    >  2:35064/1‑140 (MQ=255)                                                                                                                           cGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAGATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATcag   >  1:713758/1‑140 (MQ=255)                                                                                                                           cGCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATcag   >  2:122085/1‑140 (MQ=255)                                                                                                                            gCCGGAATTGACAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATcagc  >  1:746126/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                  AGATCAGCAAGCAACACACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCAAACGTCGACTCTCCCGGTGTATTCGGTTGCCGGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCCACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACGATCAGC  >  NC_000913/2443107‑2443369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑