Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,507,579	A→G	L106L (TTG→CTG)	fryC ←	putative PTS enzyme IIC component FryC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,507,579	0	A	G	100.0%	165.3 / NA	47	L106L (TTG→CTG)	fryC	putative PTS enzyme IIC component FryC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/21);  total (26/21)

TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAG  >  NC_000913/2507444‑2507711                                                                                                                                        |                                                                                                                                     tGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCAcg                                                                                                                                  >  1:283289/1‑140 (MQ=255)   cTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCAcgcc                                                                                                                                >  2:199727/1‑140 (MQ=255)   cTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCAcgcc                                                                                                                                >  1:1142432/1‑140 (MQ=255)   cTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATAGGAATGGCAAAACCAAACAGCAcgcc                                                                                                                                >  2:226403/1‑140 (MQ=255)    tGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGTCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCAcgccg                                                                                                                               >  2:538889/1‑140 (MQ=255)    tGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCAcgccg                                                                                                                               >  1:1003905/1‑140 (MQ=255)    tGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCAcgccg                                                                                                                               >  1:424271/1‑140 (MQ=255)           gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAgg                                                                                                                        >  1:1025547/1‑140 (MQ=255)           gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAgg                                                                                                                        >  2:143293/1‑140 (MQ=255)           gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAgg                                                                                                                        >  1:1174895/1‑140 (MQ=255)                  agcagTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGcaa                                                                                                                 >  2:316742/1‑138 (MQ=255)                         gCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAg                                                                                                          >  1:1138759/1‑140 (MQ=255)                                 tGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAAttt                                                                                                  >  2:511619/1‑140 (MQ=255)                                  gggTAGACATCAACCCGCCGATAAAACCAGTCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTta                                                                                                 <  1:538889/140‑1 (MQ=255)                                        aCATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTtaagtaag                                                                                           <  2:1174895/140‑1 (MQ=255)                                           tCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGgag                                                                                        <  1:1095361/140‑1 (MQ=255)                                           tCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGgag                                                                                        <  2:747743/140‑1 (MQ=255)                                           caaccccGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGgag                                                                                        <  2:277846/136‑1 (MQ=255)                                             aaCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGagag                                                                                      >  1:60201/1‑140 (MQ=255)                                                       tAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCg                                                                            >  2:1017788/1‑140 (MQ=255)                                                         aaaCCAGCCGAGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGga                                                                          >  2:522081/1‑140 (MQ=255)                                                                ccGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTc                                                                   <  2:424271/140‑1 (MQ=255)                                                                ccGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTc                                                                   <  1:657405/140‑1 (MQ=255)                                                                  gggAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTccc                                                                 <  2:283289/140‑1 (MQ=255)                                                                   ggAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTccc                                                                 >  2:420406/1‑139 (MQ=255)                                                                   ggAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTccc                                                                 <  1:420406/139‑1 (MQ=255)                                                                   ggAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCg                                                                <  2:481022/140‑1 (MQ=255)                                                                          cAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTaa                                                         >  1:166333/1‑140 (MQ=255)                                                                          cAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAACCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTaa                                                         <  2:501970/140‑1 (MQ=255)                                                                             tttGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAagag                                                      <  1:316742/140‑1 (MQ=255)                                                                               tGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCa                                                    >  2:86375/1‑140 (MQ=255)                                                                                gCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCAt                                                   <  2:178653/140‑1 (MQ=255)                                                                                       aTAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAag                                                        >  1:37948/1‑128 (MQ=255)                                                                                       aTAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAag                                                        <  2:37948/128‑1 (MQ=255)                                                                                       aTAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCatgat                                            >  2:371199/1‑140 (MQ=255)                                                                                               cGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGAtct                                    >  2:1073600/1‑140 (MQ=255)                                                                                                      aaaGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAgg                             <  2:1165007/140‑1 (MQ=255)                                                                                                       aaGCAGCAAACATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGa                            >  2:439202/1‑140 (MQ=255)                                                                                                              aaaCATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTaa                     >  2:389407/1‑140 (MQ=255)                                                                                                                aCATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGc                   <  2:1003905/140‑1 (MQ=255)                                                                                                                aCATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGc                   <  1:1073600/140‑1 (MQ=255)                                                                                                                aCATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGc                   <  2:748936/140‑1 (MQ=255)                                                                                                                aCATCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGc                   <  1:1017788/140‑1 (MQ=255)                                                                                                                  aTCGGAATGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCa                 >  1:682453/1‑140 (MQ=255)                                                                                                                         tGGCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATaa          >  2:118139/1‑140 (MQ=255)                                                                                                                           gCAAAACCAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGc        <  1:199727/140‑1 (MQ=255)                                                                                                                                 ccAAACAGCACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAg  <  1:139577/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAG  >  NC_000913/2507444‑2507711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑