Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,252	A→G	V170A (GTA→GCA)	dapA ←	4‑hydroxy‑tetrahydrodipicolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,252	0	A	G	100.0%	196.1 / NA	54	V170A (GTA→GCA)	dapA	4‑hydroxy‑tetrahydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/29);  total (25/29)

CATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCA  >  NC_000913/2599119‑2599383                                                                                                                                      |                                                                                                                                    cATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCAcgcg                                                                                                                               <  1:554095/140‑1 (MQ=255)     tCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTaa                                                                                                                           >  2:432270/1‑140 (MQ=255)         gcgcTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTgca                                                                                                                                   >  2:798049/1‑128 (MQ=255)             tGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTg                                                                                                                   >  2:419092/1‑140 (MQ=255)             tGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTg                                                                                                                   >  1:739197/1‑140 (MQ=255)              gCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGt                                                                                                                  >  2:1060171/1‑140 (MQ=255)               cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTgca                                                                                                                                   >  1:428455/1‑122 (MQ=255)                          gTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGAt                                                                                                      <  1:161440/140‑1 (MQ=255)                                 aaaTAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGata                                                                                               <  1:944834/140‑1 (MQ=255)                                 aaaTAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGata                                                                                               <  1:432270/140‑1 (MQ=255)                                  aaTAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGataa                                                                                              >  1:737597/1‑140 (MQ=255)                                  aaTAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGataa                                                                                              <  2:915417/140‑1 (MQ=255)                                           aTGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGata                                                                                               >  1:120826/1‑130 (MQ=255)                                           aTGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGata                                                                                               <  2:120826/130‑1 (MQ=255)                                                cGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTc                                                                                >  2:115905/1‑140 (MQ=255)                                                  cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                                              >  2:828291/1‑140 (MQ=255)                                                  cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                                              >  2:800951/1‑140 (MQ=255)                                                  cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                                              >  1:946884/1‑140 (MQ=255)                                                  cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTACCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                                              >  1:530627/1‑140 (MQ=255)                                                    cAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCa                                                                            <  1:1060171/140‑1 (MQ=255)                                                            tGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                    >  1:437646/1‑140 (MQ=255)                                                                 tCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGt                                                               >  2:517149/1‑140 (MQ=255)                                                                   cAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGttt                                                             >  2:556019/1‑140 (MQ=255)                                                                           tCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGc                                                     >  2:544045/1‑140 (MQ=255)                                                                                tcatcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATc                                                <  2:599847/140‑1 (MQ=255)                                                                                tcatcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATc                                                <  1:544045/140‑1 (MQ=255)                                                                                   tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCa                                             <  2:21433/140‑1 (MQ=255)                                                                                   tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCa                                             <  1:419092/140‑1 (MQ=255)                                                                                   tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCa                                             >  2:570413/1‑140 (MQ=255)                                                                                     gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                                           <  1:789384/140‑1 (MQ=255)                                                                                     gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                                           <  1:517149/140‑1 (MQ=255)                                                                                       cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCa                                         >  1:1050115/1‑140 (MQ=255)                                                                                       cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCa                                         >  1:459231/1‑140 (MQ=255)                                                                                       cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCa                                         >  1:411975/1‑140 (MQ=255)                                                                                       cgcTCAGCAGAACAAAACCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCa                                         >  1:909823/1‑140 (MQ=255)                                                                                          tCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTa                                      <  1:452582/140‑1 (MQ=255)                                                                                             gcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACgg                                   <  1:115905/140‑1 (MQ=255)                                                                                                 aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                               <  1:838390/140‑1 (MQ=255)                                                                                                 aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                               <  1:828291/140‑1 (MQ=255)                                                                                                 aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                               >  2:608530/1‑140 (MQ=255)                                                                                                 aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                               <  2:437646/140‑1 (MQ=255)                                                                                                 aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                               <  1:556019/140‑1 (MQ=255)                                                                                                 aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACCGGACg                               <  2:411975/140‑1 (MQ=255)                                                                                                   cAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                                           <  2:82537/126‑1 (MQ=255)                                                                                                   cAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGc                                           >  1:82537/1‑126 (MQ=255)                                                                                                   cAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGc                             >  2:477759/1‑140 (MQ=255)                                                                                                   cAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGc                             <  1:570413/140‑1 (MQ=255)                                                                                                      aaTCATCTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcaca                          <  1:800951/140‑1 (MQ=255)                                                                                                           tcTGAAACCAGCTCTTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtata                     >  1:1053061/1‑140 (MQ=255)                                                                                                                       tctTTGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCgg         <  2:780024/140‑1 (MQ=255)                                                                                                                         tttGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa       <  2:35774/140‑1 (MQ=255)                                                                                                                         tttGATCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa       <  1:608530/140‑1 (MQ=255)                                                                                                                             aTCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc   <  2:952830/140‑1 (MQ=255)                                                                                                                              tCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc   <  2:560350/139‑1 (MQ=255)                                                                                                                              tCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc   >  1:560350/1‑139 (MQ=255)                                                                                                                              tCTGGTTTGCACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCa  <  2:946884/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    CATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCA  >  NC_000913/2599119‑2599383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑