Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,657,759	G→A	P393S (CCA→TCA)	hscA ←	iron‑sulfur cluster biosynthesis chaperone HscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,657,759	0	G	A	100.0%	248.8 / NA	70	P393S (CCA→TCA)	hscA	iron‑sulfur cluster biosynthesis chaperone HscA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/30);  total (40/30)

GGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGGACGACCGAAAAATTCGCC  >  NC_000913/2657630‑2657891                                                                                                                                  |                                                                                                                                     ggATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCa                                                                                                                            <  2:1067224/140‑1 (MQ=255)   aTAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGAt                                                                                                                                   >  1:212696/1‑131 (MQ=255)   aTAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGAt                                                                                                                                   <  2:212696/131‑1 (MQ=255)   aTAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAg                                                                                                                          >  2:996163/1‑140 (MQ=255)   aTAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAg                                                                                                                          >  2:959250/1‑140 (MQ=255)   aTAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAg                                                                                                                          >  2:498101/1‑140 (MQ=255)    tAGACATCGCCGTCTGACCATCTTTAAAGGTGGTTAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATca                                                                                                                                 <  1:145850/132‑1 (MQ=255)    tAGACATCGCCGTCTGACCATCTTTAAAGGTGGTTAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATca                                                                                                                                 >  2:145850/1‑132 (MQ=255)        cATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACa                                                                                                                     >  2:363290/1‑140 (MQ=255)         aTCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTgaga                                                                                                                                    >  1:245891/1‑124 (MQ=255)         aTCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAg                                                                                                                    >  2:794158/1‑140 (MQ=255)           cGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCa                                                                                                                  >  1:715719/1‑140 (MQ=255)           cGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCa                                                                                                                  >  1:385267/1‑140 (MQ=255)            gCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCAt                                                                                                                 >  2:575203/1‑140 (MQ=255)             ccGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGTAACAGCAtt                                                                                                                >  2:399844/1‑140 (MQ=255)                  tGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCt                                                                                                           >  2:512994/1‑140 (MQ=255)                     ccATCTTTAAAGGTTGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTc                                                                                                        <  1:646680/140‑1 (MQ=255)                     ccATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTc                                                                                                        <  1:996163/140‑1 (MQ=255)                     ccATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTc                                                                                                        <  2:54071/140‑1 (MQ=255)                     ccATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTc                                                                                                        <  1:444972/140‑1 (MQ=255)                          tttAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCt                                                                                                   <  2:715719/140‑1 (MQ=255)                          tttAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGCTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCt                                                                                                   >  2:70551/1‑140 (MQ=255)                              aaGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGtt                                                                                               <  2:352842/140‑1 (MQ=255)                               aGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCCCTTTCTCCACCAGGCCGCCCACCGTTTCCAGGCCCAGCGACAGTGAGATCACATCAAGCCACAGCATTTCGCTGTCTGGCTTGGTa                                                                                              <  2:872448/140‑1 (MQ=255)                               aGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGCTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTa                                                                                              <  1:70551/140‑1 (MQ=255)                                   ggtgAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTg                                                                                                 >  2:91279/1‑133 (MQ=255)                                   ggtgAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTg                                                                                                 <  1:91279/133‑1 (MQ=255)                                   ggtgAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACcca                                                                                          >  2:407291/1‑140 (MQ=255)                                    gtgAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTa                                                                                              <  1:1076571/135‑1 (MQ=255)                                    gtgAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTa                                                                                              >  2:1076571/1‑135 (MQ=255)                                    gtgAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACccac                                                                                         >  1:581493/1‑140 (MQ=255)                                                   cgGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTg                                                                          >  1:953762/1‑140 (MQ=255)                                                     ggCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGaa                                                                        <  1:575203/140‑1 (MQ=255)                                                         aCCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATcgc                                                                    <  1:512994/140‑1 (MQ=255)                                                                tAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATcgc                                                                    >  1:249376/1‑133 (MQ=255)                                                                tAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATcgc                                                                    <  2:249376/133‑1 (MQ=255)                                                                  gTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAAt                                                           >  2:304812/1‑140 (MQ=255)                                                                     gTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGc                                                        >  1:46355/1‑140 (MQ=255)                                                                     gTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGc                                                        >  2:472937/1‑140 (MQ=255)                                                                     gTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGc                                                        >  2:332650/1‑140 (MQ=255)                                                                     gTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTGTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGc                                                        >  1:222332/1‑140 (MQ=255)                                                                       aTTACGCGGACTCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcga                                                      <  1:1173564/140‑1 (MQ=255)                                                                       aTTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcga                                                      >  1:295299/1‑140 (MQ=255)                                                                       aTTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcga                                                      <  2:581493/140‑1 (MQ=255)                                                                       aTTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcga                                                      <  1:1063929/140‑1 (MQ=255)                                                                        ttACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcgac                                                     >  1:663494/1‑140 (MQ=255)                                                                        ttACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGATACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcgac                                                     >  1:384248/1‑140 (MQ=255)                                                                                aaTCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcga                                                      >  2:175752/1‑131 (MQ=255)                                                                                aaTCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGcga                                                      <  1:175752/131‑1 (MQ=255)                                                                                aaTCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTAt                                             >  2:1131195/1‑140 (MQ=255)                                                                                      tttCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGt                                       <  1:832264/140‑1 (MQ=255)                                                                                          tcCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGAt                                   >  1:54947/1‑140 (MQ=255)                                                                                           ccaccaGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATg                                  <  2:953762/140‑1 (MQ=255)                                                                                           ccaccaGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATg                                  <  2:54947/140‑1 (MQ=255)                                                                                           ccaccaGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATg                                  <  1:794158/140‑1 (MQ=255)                                                                                                aGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGt                             <  1:332650/140‑1 (MQ=255)                                                                                                aGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGt                             <  1:407291/140‑1 (MQ=255)                                                                                                 ggCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGt                             >  1:941521/1‑139 (MQ=255)                                                                                                 ggCCGCCCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGt                             <  2:941521/139‑1 (MQ=255)                                                                                                  gccgccCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCa                           >  1:779622/1‑140 (MQ=255)                                                                                                    cgccCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGt                                       <  2:8098/126‑1 (MQ=255)                                                                                                    cgccCATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGt                                       >  1:8098/1‑126 (MQ=255)                                                                                                      cccATCGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCgg                       >  2:977474/1‑140 (MQ=255)                                                                                                           cGTTTCGAGGCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGgac                  >  1:71247/1‑140 (MQ=255)                                                                                                                   ggCCCAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGGACGACCGaaa          >  2:35102/1‑140 (MQ=255)                                                                                                                     cccAGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGGACGACCGaaaaa        >  2:389860/1‑140 (MQ=255)                                                                                                                        aGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGGACGACCGAAAAATTc     >  1:165604/1‑140 (MQ=255)                                                                                                                        aGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGGACGACCGAAAAATTc     >  1:342644/1‑140 (MQ=255)                                                                                                                        aGCGACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGGACGACCGAAAAATTc     >  2:955667/1‑140 (MQ=255)                                                                                                                           gACAGTGAGATCACATCAAGTAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTTGACGACCGAAAAATTCGcc  <  1:399844/140‑1 (MQ=255)                                                                                                                           gACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGGACGACCGAAAAATTCGcc  <  1:363290/140‑1 (MQ=255)                                                                                                                           gACAGTGAGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGGACGACCGAAAAATTCGcc  <  2:779622/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                     GGATAGACATCGCCGTCTGACCATCTTTAAAGGTGGTGAAATCCTGAGCGCGGGCCACCGGAATAGTGGTATTACGCGGAATCACTTTCTCCACCAGGCCGCCCATCGTTTCGAGGCCCAGCGACAGTGGGATCACATCAAGCAACAGCATTTCGCTGTCTGGCTTGTTACCCACCAGAATATCCGCCTGAATCGCCGCGCCAATAGCGACGACTTTATCCGGGTCGATGGAAGTCAGCGGTGGACGACCGAAAAATTCGCC  >  NC_000913/2657630‑2657891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑