Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,678,790	G→A	A193V (GCG→GTG)	yphF ←	putative ABC transporter periplasmic binding protein YphF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,790	0	G	A	100.0%	168.3 / NA	48	A193V (GCG→GTG)	yphF	putative ABC transporter periplasmic binding protein YphF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/21);  total (27/21)

GCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGC  >  NC_000913/2678655‑2678923                                                                                                                                        |                                                                                                                                      gCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGcc                                                                                                                                   <  2:28208/140‑1 (MQ=255)  cTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTgcacg                                                                                                                                     >  2:774524/1‑137 (MQ=255)       aCCGCGCCGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAAc                                                                                                                             >  1:228865/1‑140 (MQ=255)          gcgcCGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAAcgcg                                                                                                                          >  2:793654/1‑140 (MQ=255)           cgcCGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCgg                                                                                                                         >  1:349736/1‑140 (MQ=255)                            cccGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACttcttc                                                                                                        >  1:431470/1‑140 (MQ=255)                              cGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCaa                                                                                                      <  2:431470/140‑1 (MQ=255)                                        aTAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACg                                                                                            <  2:909095/140‑1 (MQ=255)                                         tAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGt                                                                                           <  1:1167350/140‑1 (MQ=255)                                           aTGGCGTTGAGATCCGGCGTGGAGATGATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGATAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCg                                                                                         <  2:1127225/140‑1 (MQ=255)                                              gCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTg                                                                                      >  2:798050/1‑140 (MQ=255)                                               cGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCa                                                                                                       <  2:993329/122‑1 (MQ=255)                                               cGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCa                                                                                                       >  1:993329/1‑122 (MQ=255)                                                gTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGca                                                                                    <  1:224446/140‑1 (MQ=255)                                                 ttGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGcac                                                                                   <  2:228865/140‑1 (MQ=255)                                                    agaTCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGcacaca                                                                                >  2:456779/1‑140 (MQ=255)                                                    agaTCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGcacaca                                                                                <  1:1118382/140‑1 (MQ=255)                                                     gaTCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACaa                                                                               <  1:646533/140‑1 (MQ=255)                                                        ccGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACt                                                                            >  2:485598/1‑140 (MQ=255)                                                           gCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCa                                                                         <  1:1019011/140‑1 (MQ=255)                                                                      aTTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCa                                                              >  2:509378/1‑140 (MQ=255)                                                                           tttttCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATtga                                                         >  1:550604/1‑140 (MQ=255)                                                                                 aCCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACgg                                                   >  1:1082634/1‑140 (MQ=255)                                                                                     aCGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAAt                                               >  1:800281/1‑140 (MQ=255)                                                                                     aCGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAAt                                               >  1:131432/1‑140 (MQ=255)                                                                                           aTCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCgg                                         <  1:793654/140‑1 (MQ=255)                                                                                                tttATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGt                                    <  1:485598/140‑1 (MQ=255)                                                                                                              tCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCGCACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCa                      >  2:952987/1‑140 (MQ=255)                                                                                                              tCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCa                      >  1:859221/1‑140 (MQ=255)                                                                                                              tCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCa                      >  1:868162/1‑140 (MQ=255)                                                                                                              tCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCa                      >  1:894591/1‑140 (MQ=255)                                                                                                              tCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCa                      <  2:131432/140‑1 (MQ=255)                                                                                                              tCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCa                      <  2:550604/140‑1 (MQ=255)                                                                                                              tCCCTTCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCa                      >  1:321121/1‑140 (MQ=255)                                                                                                                   tCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTAtt                          >  2:37855/1‑131 (MQ=255)                                                                                                                   tCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTAtt                          <  1:37855/131‑1 (MQ=255)                                                                                                                   tCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCaataaa                 <  2:503349/140‑1 (MQ=255)                                                                                                                   tCCTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCaataaa                 >  1:207597/1‑140 (MQ=255)                                                                                                                    ccTGATTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCaataaaa                >  1:125757/1‑140 (MQ=255)                                                                                                                        aTTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAaataat            <  2:207597/140‑1 (MQ=255)                                                                                                                        aTTAGCGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAaataat            >  1:1015020/1‑140 (MQ=255)                                                                                                                          tAGCGACAATTTGCACGCCGGGAACGCGGGATTTCAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCa          >  1:231776/1‑140 (MQ=255)                                                                                                                             cGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCg       >  1:570052/1‑140 (MQ=255)                                                                                                                             cGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCg       >  1:297152/1‑140 (MQ=255)                                                                                                                             cGACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCg       >  2:971853/1‑140 (MQ=255)                                                                                                                              gACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCgg      <  2:125757/140‑1 (MQ=255)                                                                                                                              gACAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCgg      <  2:117470/140‑1 (MQ=255)                                                                                                                                cAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCa    >  1:22344/1‑140 (MQ=255)                                                                                                                                cAATTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCa    <  2:570052/140‑1 (MQ=255)                                                                                                                                  aTTTGCACGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGc  <  2:297152/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      GCTTTTACCGCGCCGAGTGTCGCACCGCCCGATTCCCCCATAATGGCGTTGAGATCCGGCGTGGAGATAATTAGTTTTTCACCAACGGAAATCGCTTTATCTAAAACAGTCCCTTCCTGATTAGCGACAATTTGCGCGCCGGGAACGCGGGATTTTAAAACTTCTTCAAATCCTTTACGTCGCTGCACACAAACTTCAAAGGCTTCGCAATTGATGACGGCAATTTTCGGCTGGTCAATTTTATTGGCAATAAAATAATCAGCGGCAGC  >  NC_000913/2678655‑2678923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑