Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,719,629	T→C	L136P (CTC→CCC)	yfiP →	conserved protein YfiP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,719,629	0	T	C	100.0%	209.1 / NA	57	L136P (CTC→CCC)	yfiP	conserved protein YfiP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

CCCTCGCAGGATTTGCTGGAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTG  >  NC_000913/2719493‑2719751                                                                                                                                         |                                                                                                                           cccTCGCAGGATTTGCTGGAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGa                                                                                                                         <  1:865831/140‑1 (MQ=255)     cGCAGGATTTGCTGGAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGt                                                                                                                     <  2:387532/140‑1 (MQ=255)     cGCAGGATTTGCTGGAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGt                                                                                                                     >  2:1047098/1‑140 (MQ=255)           aTTTGCTGGAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTgg                                                                                                               >  2:79090/1‑140 (MQ=255)           aTTTGCTGGAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTgg                                                                                                               >  2:75667/1‑140 (MQ=255)                  ggAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAg                                                                                                        <  2:369210/140‑1 (MQ=255)                    aGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCt                                                                                                      >  1:566051/1‑140 (MQ=255)                    aGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCt                                                                                                      <  2:566051/140‑1 (MQ=255)                    aGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCt                                                                                                      >  1:629858/1‑140 (MQ=255)                     gTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTc                                                                                                     >  1:677725/1‑140 (MQ=255)                     gTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTc                                                                                                     >  1:200503/1‑140 (MQ=255)                       tGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGc                                                                                                   <  2:55645/140‑1 (MQ=255)                        ggTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCa                                                                                                  >  2:907731/1‑140 (MQ=255)                        ggTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCa                                                                                                  >  1:944035/1‑140 (MQ=255)                        ggTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCa                                                                                                  <  1:75667/140‑1 (MQ=255)                        ggTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCa                                                                                                  >  1:868143/1‑140 (MQ=255)                        ggTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCa                                                                                                  <  1:79090/140‑1 (MQ=255)                         gTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCaa                                                                                                 >  1:892069/1‑140 (MQ=255)                              aaaCCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCa                                                                                                  <  1:79064/134‑1 (MQ=255)                              aaaCCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCa                                                                                                  >  2:79064/1‑134 (MQ=255)                                    ggACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTa                                                                                      >  1:767635/1‑140 (MQ=255)                                      aCTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTaaa                                                                                    <  1:1047098/140‑1 (MQ=255)                                              ccAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTa                                                                            <  1:111148/140‑1 (MQ=255)                                               cAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTAt                                                                           <  2:944035/140‑1 (MQ=255)                                                aaTGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATc                                                                          <  2:892069/140‑1 (MQ=255)                                                        tCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCt                                                                         <  2:613598/133‑1 (MQ=255)                                                        tCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCt                                                                         >  1:613598/1‑133 (MQ=255)                                                           ttCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCtt                                                               <  2:164734/140‑1 (MQ=255)                                                                   tCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCAt                                                       <  1:784869/140‑1 (MQ=255)                                                                      tATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTc                                                    >  1:229234/1‑140 (MQ=255)                                                                            gatgaGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGa                                              >  2:807723/1‑140 (MQ=255)                                                                            gatgaGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGa                                              >  2:1104985/1‑140 (MQ=255)                                                                              tgaGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATc                                            >  2:965637/1‑140 (MQ=255)                                                                                  cAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTc                                        >  1:450852/1‑140 (MQ=255)                                                                                  cAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTc                                        >  1:750073/1‑140 (MQ=255)                                                                                   aaCGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTcc                                       <  2:750073/140‑1 (MQ=255)                                                                                    aCGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTccc                                      >  2:288879/1‑140 (MQ=255)                                                                                    aCGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTccc                                      >  1:824547/1‑140 (MQ=255)                                                                                    aCGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTccc                                      <  1:965637/140‑1 (MQ=255)                                                                                      gggAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTcc                                       >  1:296086/1‑137 (MQ=255)                                                                                      gggAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTcc                                       <  2:296086/137‑1 (MQ=255)                                                                                          aGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCttt                                <  2:229234/140‑1 (MQ=255)                                                                                           gTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTc                               <  2:524808/140‑1 (MQ=255)                                                                                           gTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTc                               <  1:1104985/140‑1 (MQ=255)                                                                                           gTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTc                               <  2:629858/140‑1 (MQ=255)                                                                                               tCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGcc                           >  2:228408/1‑140 (MQ=255)                                                                                                  tCACACCACCTGCCGGTAAGCCACCGCTGTTTATCCTGCCCGATGGTACCTGGCCGGGAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTAt                        <  2:810015/140‑1 (MQ=255)                                                                                                      cccaccTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGcc                    <  2:287532/139‑1 (MQ=255)                                                                                                       ccaccTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCt                   <  2:824547/140‑1 (MQ=255)                                                                                                          ccTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                >  1:864849/1‑140 (MQ=255)                                                                                                          ccTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                >  1:82102/1‑140 (MQ=255)                                                                                                          ccTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                >  1:936854/1‑140 (MQ=255)                                                                                                          ccTGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCg                >  1:672045/1‑140 (MQ=255)                                                                                                            tGCCGGTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTCCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTg              <  2:89834/140‑1 (MQ=255)                                                                                                                 gTAAGCCACCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGcc         <  2:57636/140‑1 (MQ=255)                                                                                                                        aCCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTg  >  2:735500/1‑140 (MQ=255)                                                                                                                        aCCGCTGTTTATCATGCCCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTg  <  2:1001332/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                           CCCTCGCAGGATTTGCTGGAGTTGGTGCAAAACCCGGACTATCAGCCAATGGTGGTCTTTCCCGCTTCGTATGCTGATGAGCAACGGGAAGTGATCTTCACACCACCTGCCGGTAAGCCACCGCTGTTTATCATGCTCGATGGTACCTGGCCGGAAGCTCGCAAGATGTTTCGTAAAAGTCCGTATCTGGATAATCTTCCCGTCATTTCCGTCGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTG  >  NC_000913/2719493‑2719751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑