Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	258,897	A→G	pseudogene (222/331 nt)	crl →	RNA polymerase holoenzyme assembly factor Crl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	258,897	0	A	G	100.0%	255.1 / NA	71	pseudogene (222/331 nt)	crl	RNA polymerase holoenzyme assembly factor Crl
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/28);  total (43/28)

GGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACA  >  NC_000913/258766‑259030                                                                                                                                    |                                                                                                                                      gggCTGGTGGATGGAGCTGGAAGCGC‑GGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCg                                                                                                                              <  1:447342/140‑1 (MQ=255)      ggtggATGGAGCTGGAAGCGCAGGAATCCCGTTTTACGTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTTAg                                                                                                                          >  2:1038426/1‑140 (MQ=255)       gtggATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGt                                                                                                                         >  1:404472/1‑140 (MQ=255)       gtggATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGt                                                                                                                         >  1:970878/1‑140 (MQ=255)        tggatggaGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCGCCCTGCGTGAGtt                                                                                                                        <  2:381862/140‑1 (MQ=255)               aGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACgag                                                                                                                 >  1:1129713/1‑140 (MQ=255)               aGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACgag                                                                                                                 >  2:485694/1‑140 (MQ=255)                 cTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGaa                                                                                                               <  1:248538/140‑1 (MQ=255)                           aGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGaa                                                                                                     <  2:543541/140‑1 (MQ=255)                           aGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGaa                                                                                                     <  2:863067/140‑1 (MQ=255)                                  ccGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTga                                                                                              <  1:700189/140‑1 (MQ=255)                                  ccGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTga                                                                                              <  2:1129713/140‑1 (MQ=255)                                     ttttACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTgacga                                                                                           >  2:418672/1‑140 (MQ=255)                                       ttACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGacgacg                                                                                         >  1:286652/1‑140 (MQ=255)                                       ttACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGacgacg                                                                                         >  1:1114499/1‑140 (MQ=255)                                        tACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGc                                                                                        <  1:481875/140‑1 (MQ=255)                                              cAGTTA‑CAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCt                                                                                 >  1:1016297/1‑140 (MQ=255)                                                 ttACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAAt                                                                                   >  2:107980/1‑136 (MQ=255)                                                 ttACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAAt                                                                                   <  1:107980/136‑1 (MQ=255)                                                    ccAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGc                                                                            >  1:528436/1‑140 (MQ=255)                                                    ccAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGc                                                                            >  1:468491/1‑140 (MQ=255)                                                     cAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCt                                                                           >  1:857650/1‑140 (MQ=255)                                                      aGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTg                                                                          <  2:528436/140‑1 (MQ=255)                                                           ggTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAAcc                                                                     >  1:910781/1‑140 (MQ=255)                                                             tCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAAc                                                                      <  2:547832/137‑1 (MQ=255)                                                             tCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACcgg                                                                   >  2:688575/1‑140 (MQ=255)                                                             tCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGGGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAAc                                                                      >  1:547832/1‑137 (MQ=255)                                                                 ttCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGga                                                               >  1:318761/1‑140 (MQ=255)                                                                 ttCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGga                                                               <  2:1061538/140‑1 (MQ=255)                                                                 ttCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGga                                                               >  1:1160049/1‑140 (MQ=255)                                                                    gATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGgatga                                                            <  1:294965/140‑1 (MQ=255)                                                                      tAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGAtt                                                          >  2:624518/1‑140 (MQ=255)                                                                      tAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGAtt                                                          >  1:145151/1‑140 (MQ=255)                                                                           cAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGt                                                     >  1:222813/1‑140 (MQ=255)                                                                             ggCGACTGGAAGAGTGTTCCGGTAAAAGCCCCGGAAGTGGTTGAACGACTGGAGCCCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCGGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGa                                                   <  2:857650/140‑1 (MQ=255)                                                                              gCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGAc                                                  >  1:506803/1‑140 (MQ=255)                                                                                  cTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTTAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGc                                              >  2:909091/1‑140 (MQ=255)                                                                                   tGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGcc                                             >  1:504693/1‑140 (MQ=255)                                                                                    ggAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCg                                            <  2:1114499/140‑1 (MQ=255)                                                                                      aaGAGTGTTCGGGTAAAAGACACGGAAGTGGTTGAACGCTTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAATTGATGACGACGCTGACTCTGAAGCTGGAACCGGCGGTTGTTTTCGGTGACGTGACGGt                                          <  2:855782/140‑1 (MQ=255)                                                                                      aaGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGt                                          <  2:468491/140‑1 (MQ=255)                                                                                      aaGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGt                                          <  1:289067/140‑1 (MQ=255)                                                                                        gagTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTTAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGa                                        <  1:1038426/140‑1 (MQ=255)                                                                                        gagTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGa                                        >  2:102199/1‑140 (MQ=255)                                                                                        gagTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGa                                        >  1:767153/1‑140 (MQ=255)                                                                                           tgtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGt                                     >  2:427715/1‑140 (MQ=255)                                                                                            gtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGt                                          >  2:939589/1‑134 (MQ=255)                                                                                            gtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGt                                          <  1:939589/134‑1 (MQ=255)                                                                                            gtTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGtt                                    >  1:855782/1‑140 (MQ=255)                                                                                             ttCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTa                                   >  2:46015/1‑140 (MQ=255)                                                                                                  gTAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGc                              >  1:603041/1‑140 (MQ=255)                                                                                                   tAAAAGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCg                             >  1:745040/1‑140 (MQ=255)                                                                                                    aaaaGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTACTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCgt                            >  1:791113/1‑140 (MQ=255)                                                                                                     aaaGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCgtg                           >  2:732996/1‑140 (MQ=255)                                                                                                     aaaGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCgtg                           >  2:636631/1‑140 (MQ=255)                                                                                                       aGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCgtgag                         >  2:790735/1‑140 (MQ=255)                                                                                                       aGACACTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCgtgag                         >  2:559833/1‑140 (MQ=255)                                                                                                           acTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGaa                     <  1:790735/140‑1 (MQ=255)                                                                                                            cTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACgg                               >  1:943172/1‑129 (MQ=255)                                                                                                            cTGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACgg                               <  2:943172/129‑1 (MQ=255)                                                                                                             tGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCgtgagtga                      >  1:586339/1‑137 (MQ=255)                                                                                                             tGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCgtgagtga                      <  2:586339/137‑1 (MQ=255)                                                                                                             tGAAGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAAt                   <  1:418672/140‑1 (MQ=255)                                                                                                                aGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAAtgtg                <  2:992942/140‑1 (MQ=255)                                                                                                                aGTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGCGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAAtgtg                <  2:286652/140‑1 (MQ=255)                                                                                                                 gTGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGc               >  1:990923/1‑140 (MQ=255)                                                                                                                  tGGTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGcc              >  2:437184/1‑140 (MQ=255)                                                                                                                   ggTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCg             <  2:145151/140‑1 (MQ=255)                                                                                                                    gTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCgg            >  1:304935/1‑140 (MQ=255)                                                                                                                    gTTGAACGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGACGGTGAAGTTAACGGCGTGAGTGAAATGTGCCgg            >  1:985330/1‑140 (MQ=255)                                                                                                                          cGACTGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCAt      <  2:318761/140‑1 (MQ=255)                                                                                                                              tGGAGCGCACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATcaca  <  1:559833/140‑1 (MQ=255)                                                                                                                              tGGAGCGCACCCTGCGAGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATcaca  <  2:1052940/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      GGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGTGAGTTTCACGAGAAGCTGCGTGAACTGCTGACGACGCTGAATCTGAAGCTGGAACCGGCGGATGATTTTCGTGACGAGCCGGTGAAGTTAACGGCGTGAGTGAAATGTGCCGGATGCATCACA  >  NC_000913/258766‑259030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑