Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,793,987	G→A	S418N (AGC→AAC)	gabT →	4‑aminobutyrate aminotransferase GabT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,793,987	0	G	A	100.0%	201.4 / NA	59	S418N (AGC→AAC)	gabT	4‑aminobutyrate aminotransferase GabT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/27);  total (32/27)

CACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAA  >  NC_000913/2793850‑2794122                                                                                                                                          |                                                                                                                                        cACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAAcc                                                                                                                                       <  2:856205/140‑1 (MQ=255)  aCCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCa                                                                                                                                      <  1:721883/140‑1 (MQ=255)                       gcCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCa                                                                                                                 >  1:271407/1‑140 (MQ=255)                              aTAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAgcgc                                                                                                          <  2:271407/140‑1 (MQ=255)                                     ccTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCt                                                                                                   <  2:147202/140‑1 (MQ=255)                                              tctctcCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAg                                                                                          >  2:216732/1‑140 (MQ=255)                                               ctctcCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAgg                                                                                         <  1:1043145/140‑1 (MQ=255)                                                     tGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGc                                                                                   >  1:892291/1‑140 (MQ=255)                                                     tGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGc                                                                                   >  2:730344/1‑140 (MQ=255)                                                           ccGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGcgcg                                                                             <  2:799991/140‑1 (MQ=255)                                                                      cGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGc                                                                  >  1:985733/1‑140 (MQ=255)                                                                      cGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGc                                                                  >  1:94359/1‑140 (MQ=255)                                                                      cGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGc                                                                  >  1:611957/1‑140 (MQ=255)                                                                       gTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGcc                                                                 <  1:216732/140‑1 (MQ=255)                                                                        tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTgc                                                                                >  2:21110/1‑124 (MQ=255)                                                                        tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTgc                                                                                <  1:21110/124‑1 (MQ=255)                                                                        tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCt                                                                >  2:108293/1‑140 (MQ=255)                                                                        tgctgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCt                                                                >  2:345865/1‑140 (MQ=255)                                                                           tgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                             <  1:468268/140‑1 (MQ=255)                                                                           tgcGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACg                                                             >  1:378183/1‑140 (MQ=255)                                                                            gcgcATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACgg                                                            <  1:730344/140‑1 (MQ=255)                                                                                 tCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATc                                                       <  1:108293/140‑1 (MQ=255)                                                                                 tCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATc                                                       <  1:345865/140‑1 (MQ=255)                                                                                     tGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGTGGATCCAgg                                                   <  2:215365/140‑1 (MQ=255)                                                                                     tGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAgg                                                   <  2:611957/140‑1 (MQ=255)                                                                                     tGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAgg                                                   <  2:985733/140‑1 (MQ=255)                                                                                     tGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAgg                                                   <  2:94359/140‑1 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGAt                                                        <  2:95861/128‑1 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGAt                                                        >  1:95861/1‑128 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCGGGTCGGATa                                            >  1:1047294/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  2:2732/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  2:934828/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  2:1026176/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  2:176529/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  2:159698/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  1:138507/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  1:410280/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  1:451245/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  1:512984/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  1:1150419/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  1:521232/1‑140 (MQ=255)                                                                                            cTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATa                                            >  1:798771/1‑140 (MQ=255)                                                                                                    tGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGttt                                    >  2:478415/1‑140 (MQ=255)                                                                                                             tCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCAt                           <  1:478415/140‑1 (MQ=255)                                                                                                             tCAGATCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCAt                           <  2:549679/140‑1 (MQ=255)                                                                                                                 aTCCGTCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGa                       <  2:892291/140‑1 (MQ=255)                                                                                                                      tCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                  >  2:531582/1‑140 (MQ=255)                                                                                                                      tCAGGGTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                  <  1:254794/140‑1 (MQ=255)                                                                                                                           gTCTGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTAc             >  2:473719/1‑140 (MQ=255)                                                                                                                              tGGAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTg          >  1:234280/1‑140 (MQ=255)                                                                                                                               ggAGATCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGa         >  2:56822/1‑140 (MQ=255)                                                                                                                                 agaTCATCAACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAAc       <  2:451245/140‑1 (MQ=255)                                                                                                                                    tcatcaACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAgg    <  1:2732/140‑1 (MQ=255)                                                                                                                                     catcaACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                  <  2:719643/125‑1 (MQ=255)                                                                                                                                     catcaACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATc                  >  1:719643/1‑125 (MQ=255)                                                                                                                                     catcaACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGga   >  2:988689/1‑140 (MQ=255)                                                                                                                                      atcaACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa  <  1:176529/140‑1 (MQ=255)                                                                                                                                      atcaACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa  <  2:234280/140‑1 (MQ=255)                                                                                                                                      atcaACCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGgaa  <  2:138507/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CACCGCCGAGATCGTGGCTCGCGCCCGCGATAAAGGCCTGATTCTTCTCTCCTGCGGCCCGTATTACAACGTGCTGCGCATCCTTGTACCGCTCACCATTGAAGACGCTCAGATCCGTCAGGGTCTGGAGATCATCAGCCAGTGTTTTGATGAGGCGAAGCAGTAGCGCCGCTCCTATGCCGGAGGCGACGCTGCGCGTCTTGTCCGGCCTACGGGGATCCAGGTCGGATAAGGCGTTTACGCCGCATCCGACAATCTGTACGTGAACAGGAA  >  NC_000913/2793850‑2794122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑