Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,805,945	A→G	Q377Q (CAA→CAG)	proV →	glycine betaine ABC transporter ATP binding subunit ProV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,805,945	0	A	G	100.0%	169.1 / NA	47	Q377Q (CAA→CAG)	proV	glycine betaine ABC transporter ATP binding subunit ProV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/30);  total (17/30)

ACGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCAATCCGCAGACGCC  >  NC_000913/2805814‑2806078                                                                                                                                    |                                                                                                                                      aCGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGt                                                                                                                               <  2:678219/140‑1 (MQ=255) aCGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGt                                                                                                                               <  2:391748/140‑1 (MQ=255)  cGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTc                                                                                                                              <  2:910484/140‑1 (MQ=255)     agcagcaAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGc                                                                                                                           <  1:878688/140‑1 (MQ=255)      gcagcaAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGGATGTCGGca                                                                                                                          >  1:1067756/1‑140 (MQ=255)       cagcaAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGcat                                                                                                                         >  1:478084/1‑140 (MQ=255)            aGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGc                                                                                                                           <  1:1077092/133‑1 (MQ=255)            aGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGc                                                                                                                           >  2:1077092/1‑133 (MQ=255)                         gcgcTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAAtgct                                                                                                       >  2:1015411/1‑140 (MQ=255)                          cgcTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAAtgctg                                                                                                      <  2:478084/140‑1 (MQ=255)                              gattgatGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAAtgctgctgc                                                                                                  <  2:458388/140‑1 (MQ=255)                              gattgatGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAAtgctgctgc                                                                                                  <  1:746590/140‑1 (MQ=255)                                ttgatGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTgcgc                                                                                                <  1:1130675/140‑1 (MQ=255)                                    tGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCtt                                                                                            >  1:518268/1‑140 (MQ=255)                                     gcgcCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCttt                                                                                           <  2:474798/140‑1 (MQ=255)                                      cgcCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTa                                                                                          <  1:1032950/140‑1 (MQ=255)                                      cgcCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTa                                                                                          >  1:397284/1‑140 (MQ=255)                                      cgcCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTa                                                                                          <  1:449109/140‑1 (MQ=255)                                           ttAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCg                                                                                     >  2:287920/1‑140 (MQ=255)                                             aGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCGCCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTg                                                                                   <  2:83392/140‑1 (MQ=255)                                               cAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAg                                                                                 <  2:518268/140‑1 (MQ=255)                                               cAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAg                                                                                 <  1:287920/140‑1 (MQ=255)                                                aGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAgg                                                                                <  1:738270/140‑1 (MQ=255)                                                   cGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAggggg                                                                             <  2:954803/140‑1 (MQ=255)                                                   cGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAggggg                                                                             <  2:129080/140‑1 (MQ=255)                                                   cGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAggggg                                                                             >  1:587517/1‑140 (MQ=255)                                                     aTGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAg                                                                                 >  2:587880/1‑134 (MQ=255)                                                     aTGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAg                                                                                 <  1:587880/134‑1 (MQ=255)                                                     aTGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTa                                                                           <  1:761694/140‑1 (MQ=255)                                                            aaCGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATg                                                                    >  1:118225/1‑140 (MQ=255)                                                             aCGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATgg                                                                   <  2:854526/140‑1 (MQ=255)                                                             aCGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATgg                                                                   <  2:930162/140‑1 (MQ=255)                                                               gCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCt                                                                 <  1:1159502/140‑1 (MQ=255)                                                                  tctTAGCGAGTTGCTCTCTCATGTCGGACAGGCGCCCTGTGCGGTGCCCGTTGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGAt                                                              <  2:773153/140‑1 (MQ=255)                                                                   ctTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATc                                                             >  1:18622/1‑140 (MQ=255)                                                                           gTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAaataat                                                     <  2:18622/140‑1 (MQ=255)                                                                            ttGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCCTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATc                                                    <  1:788654/140‑1 (MQ=255)                                                                               ctctctCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGt                                                 >  1:1023639/1‑140 (MQ=255)                                                                                  tctcATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTggg                                              >  1:777725/1‑140 (MQ=255)                                                                                         tCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCAc                                       >  2:1084771/1‑140 (MQ=255)                                                                                         tCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGAAAATAATGGCTGATCAAAATAATCCGTGGGATACAAc                                       >  1:462728/1‑140 (MQ=255)                                                                                              cAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAg                                  >  2:438255/1‑140 (MQ=255)                                                                                                            ggTGCCCGTGGTCGACGAGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGcc                    <  1:644250/140‑1 (MQ=255)                                                                                                                         gacgaGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCAATCCGCAg       <  2:1132336/140‑1 (MQ=255)                                                                                                                          acgaGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCaa              >  1:594292/1‑132 (MQ=255)                                                                                                                          acgaGGACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCaa              <  2:594292/132‑1 (MQ=255)                                                                                                                              ggACCAGCAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCAATCCGCAGACGcc  <  2:507984/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      ACGCAGCAGCAAGGTCTTGATGCGGCGCTGATTGATGCGCCGTTAGCAGTCGATGCACAAACGCCTCTTAGCGAGTTGCTCTCTCATGTCGGACAGGCACCCTGTGCGGTGCCCGTGGTCGACGAGGACCAACAGTATGTCGGCATCATTTCGAAAGGAATGCTGCTGCGCGCTTTAGATCGTGAGGGGGTAAATAATGGCTGATCAAAATAATCCGTGGGATACCACGCCAGCGGCGGACAGTGCCGCGCAATCCGCAGACGCC  >  NC_000913/2805814‑2806078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑