Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,821,486	T→C	T176A (ACT→GCT)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,821,486	0	T	C	100.0%	193.4 / NA	53	T176A (ACT→GCT)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/30);  total (23/30)

CGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCTTTCATAGACGG  >  NC_000913/2821350‑2821620                                                                                                                                         |                                                                                                                                       cGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgcgtt                                                                                                                                     <  2:589724/140‑2 (MQ=255)    ttGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCAcc                                                                                                                                  <  2:305711/140‑1 (MQ=255)      gAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCa                                                                                                                                <  2:309406/140‑1 (MQ=255)       aaCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCAt                                                                                                                               <  1:795588/140‑1 (MQ=255)               gAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGa                                                                                                                       <  2:452221/140‑1 (MQ=255)                        gTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCACCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAg                                                                                                              <  1:656219/140‑1 (MQ=255)                         ttCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGa                                                                                                             <  2:1075129/140‑1 (MQ=255)                          tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGAt                                                                                                            <  2:1110416/140‑1 (MQ=255)                          tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGAt                                                                                                            >  2:584561/1‑140 (MQ=255)                               aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTa                                                                                                       <  1:941837/140‑1 (MQ=255)                                    aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGgcg                                                                                                  <  2:28773/140‑1 (MQ=255)                                    aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGgcg                                                                                                  <  2:1110138/140‑1 (MQ=255)                                    aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGgcg                                                                                                  <  1:584561/140‑1 (MQ=255)                                         aGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCt                                                                                             >  1:350185/1‑140 (MQ=255)                                           cGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCttt                                                                                           >  2:664036/1‑140 (MQ=255)                                           cGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCttt                                                                                           >  2:185579/1‑140 (MQ=255)                                                       tcttcCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt                                                                               <  1:244478/140‑1 (MQ=255)                                                       tcttcCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt                                                                               <  2:350185/140‑1 (MQ=255)                                                        cttcCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATg                                                                              <  1:664036/140‑1 (MQ=255)                                                                   cccGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACg                                                                   >  2:279496/1‑140 (MQ=255)                                                                   cccGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACg                                                                   >  1:428739/1‑140 (MQ=255)                                                                   cccGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACg                                                                   >  1:383505/1‑140 (MQ=255)                                                                       gAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcg                                                               <  1:279496/140‑1 (MQ=255)                                                                            cccccccAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCgg                                                          >  2:224655/1‑140 (MQ=255)                                                                            cccccccAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTCCGCGCgg                                                          >  2:61220/1‑140 (MQ=255)                                                                             ccccccAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcgcg                                                           <  2:1056555/138‑1 (MQ=255)                                                                             ccccccAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcgcg                                                           >  1:1056555/1‑138 (MQ=255)                                                                              cccccAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGa                                                        <  1:306441/140‑1 (MQ=255)                                                                                     aTGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTAc                                                 <  1:314089/140‑1 (MQ=255)                                                                                          gTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTActtctt                                            >  2:911986/1‑140 (MQ=255)                                                                                          gTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTActtctt                                            >  2:849854/1‑140 (MQ=255)                                                                                           tCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCttt                                           >  2:618100/1‑140 (MQ=255)                                                                                                  gATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAg                                    <  2:428739/140‑1 (MQ=255)                                                                                                               ttCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTa                            >  1:669626/1‑135 (MQ=255)                                                                                                               ttCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTa                            <  2:669626/135‑1 (MQ=255)                                                                                                                tCGGTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTtc                      >  2:1063095/1‑140 (MQ=255)                                                                                                                  ggTGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTtcgt                    >  2:695412/1‑140 (MQ=255)                                                                                                                    tGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCt                        >  1:757166/1‑134 (MQ=255)                                                                                                                    tGCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCt                        <  2:757166/134‑1 (MQ=255)                                                                                                                     gCACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTtcgtcgt                 <  1:695412/140‑1 (MQ=255)                                                                                                                      cACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTtcgtcgtc                >  2:195146/1‑140 (MQ=255)                                                                                                                      cACGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTtcgtcgtc                <  2:1158659/140‑1 (MQ=255)                                                                                                                        cGGGCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGc              >  2:886419/1‑140 (MQ=255)                                                                                                                         gggCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTtcgtcgt                 <  2:264173/136‑1 (MQ=255)                                                                                                                         gggCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTtcgtcgt                 >  1:264173/1‑136 (MQ=255)                                                                                                                         gggCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCt             <  1:224655/140‑1 (MQ=255)                                                                                                                           gCCGCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCttt           >  1:27570/1‑140 (MQ=255)                                                                                                                              gCACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCTTTCAt        <  2:458752/140‑1 (MQ=255)                                                                                                                               cACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCTTTCATa       <  1:1063095/140‑1 (MQ=255)                                                                                                                               cACGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCTTTCATa       <  1:849854/140‑1 (MQ=255)                                                                                                                                aCGGACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCTTTCATAg      >  2:957152/1‑140 (MQ=255)                                                                                                                                   gACCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCTTTCATAGACg   >  1:723814/1‑140 (MQ=255)                                                                                                                                    aCCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCTTTCATAGACgg  <  1:618100/140‑1 (MQ=255)                                                                                                                                    aCCAGCGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCTTTCATAGACgg  <  2:991223/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCCCAGATTTCGTAGGCTTCGTCGTCGCTTTCATAGACGG  >  NC_000913/2821350‑2821620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑