Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,826,079	T→C	M83T (ATG→ACG)	srlA →	sorbitol‑specific PTS enzyme IIC2 component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,826,079	0	T	C	100.0%	270.0 / NA	75	M83T (ATG→ACG)	srlA	sorbitol‑specific PTS enzyme IIC2 component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/43);  total (32/43)

CTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTG  >  NC_000913/2825943‑2826210                                                                                                                                         |                                                                                                                                    ctgGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCgacgac                                                                                                                                  >  1:565076/1‑140 (MQ=255)   ggTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGAccc                                                                                                                                <  1:661221/140‑1 (MQ=255)    gTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCt                                                                                                                               >  1:324522/1‑140 (MQ=255)                      aTTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTAt                                                                                                             >  1:1136044/1‑140 (MQ=255)                       ttAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATg                                                                                                            >  1:183340/1‑140 (MQ=255)                           ttttATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCgg                                                                                                        >  2:90668/1‑140 (MQ=255)                             ttATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGaa                                                                                                      <  1:230527/140‑1 (MQ=255)                              tATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGaaa                                                                                                     <  1:813654/140‑1 (MQ=255)                                  ggTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTa                                                                                                 <  1:980041/140‑1 (MQ=255)                                  ggTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTa                                                                                                 <  1:592091/140‑1 (MQ=255)                                  ggTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTa                                                                                                 <  2:1136044/140‑1 (MQ=255)                                   gTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTAc                                                                                                <  2:938231/140‑1 (MQ=255)                                     cAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACaa                                                                                              >  2:866332/1‑140 (MQ=255)                                      aGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGaa                                                                                                      >  2:671514/1‑131 (MQ=255)                                      aGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGaa                                                                                                      <  1:671514/131‑1 (MQ=255)                                       gCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAAc                                                                                            <  1:262688/140‑1 (MQ=255)                                             tATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGct                                                                                      >  1:302949/1‑140 (MQ=255)                                             tATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGct                                                                                      >  2:753642/1‑140 (MQ=255)                                             tATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGct                                                                                      >  1:10882/1‑140 (MQ=255)                                             tATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGct                                                                                      >  1:173670/1‑140 (MQ=255)                                                    cGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTAcgc                                                                               >  1:644157/1‑140 (MQ=255)                                                      ttttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcgg                                                                             <  1:365061/140‑1 (MQ=255)                                                      ttttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcgg                                                                             <  2:183340/140‑1 (MQ=255)                                                       tttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggc                                                                            >  2:542074/1‑140 (MQ=255)                                                        ttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                                                                           <  2:352871/140‑1 (MQ=255)                                                        ttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                                                                           <  2:908124/140‑1 (MQ=255)                                                        ttGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                                                                           <  1:154230/140‑1 (MQ=255)                                                         tGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGcggcgg                                                                          <  2:644157/140‑1 (MQ=255)                                                         tGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGcggcgg                                                                          >  2:1095158/1‑140 (MQ=255)                                                                cGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAGGTACAAACCCAGCTACTACGCGGCGGCCTCTta                                                                   >  2:125882/1‑140 (MQ=255)                                                                      gcCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTg                                                             <  2:484197/140‑1 (MQ=255)                                                                        cGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGcc                                                           <  2:150479/140‑1 (MQ=255)                                                                          gTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                                                                           <  2:282727/122‑1 (MQ=255)                                                                          gTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACgcggcg                                                                           >  1:282727/1‑122 (MQ=255)                                                                          gTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCAc                                                         >  1:723164/1‑140 (MQ=255)                                                                           tAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACt                                                        <  1:802/140‑1 (MQ=255)                                                                           tAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACt                                                        <  2:36608/140‑1 (MQ=255)                                                                            aaCCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAGCCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTc                                                       <  2:828482/140‑1 (MQ=255)                                                                                          ttACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctct                                         <  1:933997/140‑1 (MQ=255)                                                                                          ttACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctct                                         <  1:272528/140‑1 (MQ=255)                                                                                             ccTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTcc                                      >  2:749333/1‑140 (MQ=255)                                                                                             ccTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTcc                                      <  1:866332/140‑1 (MQ=255)                                                                                             ccTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTcc                                      <  2:676476/140‑1 (MQ=255)                                                                                              cTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTccc                                     <  2:173670/140‑1 (MQ=255)                                                                                                    ttACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCatat                               <  1:73434/140‑1 (MQ=255)                                                                                                    ttACCATGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCatat                               >  2:984749/1‑140 (MQ=255)                                                                                                     tACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATc                              >  1:502446/1‑140 (MQ=255)                                                                                                      aCCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTccc                                     <  1:126008/132‑1 (MQ=255)                                                                                                      aCCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTccc                                     >  2:126008/1‑132 (MQ=255)                                                                                                      aCCGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCa                             >  2:780855/1‑140 (MQ=255)                                                                                                       ccGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGTCCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctc                                          <  2:755730/126‑1 (MQ=255)                                                                                                       ccGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGTCCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCctc                                          >  1:755730/1‑126 (MQ=255)                                                                                                       ccGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTcccc                                    >  1:9359/1‑132 (MQ=255)                                                                                                       ccGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTcccc                                    <  2:9359/132‑1 (MQ=255)                                                                                                       ccGTGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCaa                            <  2:302949/140‑1 (MQ=255)                                                                                                          tGCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAAccc                         >  2:798013/1‑140 (MQ=255)                                                                                                           gCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTccc                                     <  2:397091/127‑1 (MQ=255)                                                                                                           gCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTccc                                     >  1:397091/1‑127 (MQ=255)                                                                                                           gCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCt                        >  1:856576/1‑140 (MQ=255)                                                                                                           gCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCt                        >  2:63397/1‑140 (MQ=255)                                                                                                           gCATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCt                        >  1:441245/1‑140 (MQ=255)                                                                                                            cATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTg                       >  1:618083/1‑140 (MQ=255)                                                                                                            cATTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTg                       >  2:753179/1‑140 (MQ=255)                                                                                                             aTTGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTgg                      <  1:984749/140‑1 (MQ=255)                                                                                                               tGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCg                    <  2:1132732/140‑1 (MQ=255)                                                                                                               tGGCACGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCg                    >  2:315395/1‑140 (MQ=255)                                                                                                                   aCGTTTGTCTTTTGCAATCCGACGACTCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACt                <  1:63397/140‑1 (MQ=255)                                                                                                                   aCGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACt                <  2:441245/140‑1 (MQ=255)                                                                                                                   aCGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACt                <  2:207433/140‑1 (MQ=255)                                                                                                                   aCGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACt                <  2:618083/140‑1 (MQ=255)                                                                                                                   aCGTTTGTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGACTCTTCCCCCATATCAACCCTGGCGAACt                <  2:856576/140‑1 (MQ=255)                                                                                                                         gTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCTCCATATCAACCCTGGCGAACtgtttgt          <  1:1095158/140‑1 (MQ=255)                                                                                                                         gTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACtgtttgt          <  2:10882/140‑1 (MQ=255)                                                                                                                         gTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACtgtttgt          <  1:798013/140‑1 (MQ=255)                                                                                                                         gTCTTTTGCAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACtgtttgt          <  1:780855/140‑1 (MQ=255)                                                                                                                                 cAATCCGACGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTg  >  1:1094771/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    CTGGTTATCATGAACGCACTGATTAATTTTATCGGTCAGCATCGTATTGAACGTTTTGCTCAACGTTGCGCCGGTAACCCTGTTTCCCGTTACCTACTGTTACCGTGCATTGGCACGTTTGTCTTTTGCAATCCGATGACCCTAAGCCTGGGTCGCTTTATGCCGGAAAAGTACAAACCCAGCTACTACGCGGCGGCCTCTTATAGCTGCCACTCAATGAATGGCCTCTTCCCCCATATCAACCCTGGCGAACTGTTTGTTTATCTTG  >  NC_000913/2825943‑2826210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑