| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,829,459 | T→C | I138T (ATC→ACC) | srlR → | DNA‑binding transcriptional repressor SrlR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,829,459 | 0 | T | C | 98.3% | 214.2 / ‑4.3 | 60 | I138T (ATC→ACC) | srlR | DNA‑binding transcriptional repressor SrlR |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (35/24); total (36/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-01 | |||||||||||
CGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAAC > NC_000913/2829322‑2829594 | cGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTAccc > 2:65634/1‑140 (MQ=255)cGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTAccc > 1:955780/1‑140 (MQ=255) aTCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGcc < 1:588252/140‑1 (MQ=255) aTCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGcc > 2:781132/1‑140 (MQ=255) cATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAg > 1:179035/1‑140 (MQ=255) tGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGt > 1:1080446/1‑140 (MQ=255) ggCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCg > 2:623366/1‑140 (MQ=255) ggCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCg > 1:450951/1‑140 (MQ=255) ggCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCg > 2:1152224/1‑140 (MQ=255) ttttGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCg > 2:324707/1‑140 (MQ=255) ttttGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCg > 2:11773/1‑140 (MQ=255) tttGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCgg < 2:779531/140‑1 (MQ=255) tttGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCgg < 1:1152224/140‑1 (MQ=255) gCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCt < 1:781132/140‑1 (MQ=255) gCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCt < 2:855218/140‑1 (MQ=255) cAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTc > 2:635129/1‑140 (MQ=255) aTGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCGACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCAtt > 1:576870/1‑140 (MQ=255) ggTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTc > 2:29943/1‑140 (MQ=255) tGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCaa < 1:312806/140‑1 (MQ=255) tGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCaa < 1:635129/140‑1 (MQ=255) ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACt > 1:349888/1‑140 (MQ=255) ctctCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACt > 1:40661/1‑140 (MQ=255) ctcGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTgg > 1:458414/1‑140 (MQ=255) ctcGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTgg < 1:623366/140‑1 (MQ=255) gcgcTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCag > 2:132625/1‑140 (MQ=255) cgcTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCaga < 1:29943/140‑1 (MQ=255) ttAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAAt < 2:1085326/140‑1 (MQ=255) tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAg > 1:1043617/1‑140 (MQ=255) tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCAAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCt > 1:1044838/1‑135 (MQ=255) tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCAAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCt < 2:1044838/135‑1 (MQ=255) tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCAAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCt < 2:213159/135‑1 (MQ=255) tCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCAAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCt > 1:213159/1‑135 (MQ=255) aCGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCa > 1:1145726/1‑140 (MQ=255) tgaCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCAcc > 1:16744/1‑140 (MQ=255) tgaCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCAcc > 1:277275/1‑140 (MQ=255) aCCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCtt > 2:528973/1‑140 (MQ=255) aCAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGAt < 1:528973/140‑1 (MQ=255) aCAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGAt > 1:377113/1‑140 (MQ=255) cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATa > 1:198941/1‑140 (MQ=255) cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATa > 2:1054152/1‑140 (MQ=255) cAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATa > 1:759349/1‑140 (MQ=255) ccTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAAt < 1:777216/140‑1 (MQ=255) cATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGtt > 1:101502/1‑140 (MQ=255) tatCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTa < 2:388051/140‑1 (MQ=255) gTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATggg < 2:179035/140‑1 (MQ=255) gTCAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATggg > 2:509760/1‑140 (MQ=255) cAATGCGCTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCa > 2:37426/1‑140 (MQ=255) aTGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCAcc > 1:689665/1‑140 (MQ=255) gcTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACg < 2:16744/140‑1 (MQ=255) cTATCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACgg > 1:1086026/1‑140 (MQ=255) aTCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCa > 2:107354/1‑140 (MQ=255) tCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCAt < 2:902893/140‑1 (MQ=255) tCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCAt < 1:1166492/140‑1 (MQ=255) tCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCAt < 2:40661/140‑1 (MQ=255) tCCGAACTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCAt < 1:132625/140‑1 (MQ=255) aCTGGATAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGAtc > 2:766029/1‑140 (MQ=255) aTAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAAt > 2:88291/1‑140 (MQ=255) tAACGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATg < 2:416136/140‑1 (MQ=255) cGAACAAACTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCgg > 1:820647/1‑140 (MQ=255) aaaCTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTa > 2:334129/1‑140 (MQ=255) aCTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAac < 1:1068584/140‑1 (MQ=255) aCTACCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAac < 1:65634/140‑1 (MQ=255) | CGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAAC > NC_000913/2829322‑2829594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |