Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,066,383	T→C	H264R (CAC→CGC)	ygfI ←	putative LysR‑type DNA‑binding transcriptional regulator YgfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,066,383	0	T	C	100.0%	318.4 / NA	87	H264R (CAC→CGC)	ygfI	putative LysR‑type DNA‑binding transcriptional regulator YgfI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/40);  total (47/40)

TAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGG  >  NC_000913/3066247‑3066519                                                                                                                                         |                                                                                                                                          taatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGcgcg                                                                                                                                        >  2:936546/1‑140 (MQ=255) taatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGcgcg                                                                                                                                        <  1:1142776/140‑1 (MQ=255)  aatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCgg                                                                                                                                       <  2:709806/140‑1 (MQ=255)  aatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCgg                                                                                                                                       <  2:5508/140‑1 (MQ=255)  aatttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCgg                                                                                                                                       >  1:621667/1‑140 (MQ=255)    tttaattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAT‑AAGATATTCCTGCATATACATCGGCCTGCGCGGTGc                                                                                                                                    >  2:75677/1‑140 (MQ=255)      taattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCa                                                                                                                                   >  2:105206/1‑140 (MQ=255)       aattGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCAt                                                                                                                                  <  2:934632/140‑1 (MQ=255)              aCCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAata                                                                                                                           >  1:1107436/1‑140 (MQ=255)                     cGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGc                                                                                                                    <  1:354451/140‑1 (MQ=255)                       aTGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGt                                                                                                                  >  2:264143/1‑140 (MQ=255)                                  aTGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCa                                                                                                      >  2:635954/1‑140 (MQ=255)                                  aTGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCa                                                                                                      >  1:717149/1‑140 (MQ=255)                                  aTGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCa                                                                                                      >  2:822031/1‑140 (MQ=255)                                       tCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGt                                                                                                 >  2:83354/1‑140 (MQ=255)                                          tCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGc                                                                                              <  1:83354/140‑1 (MQ=255)                                                    aTCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAAt                                                                                    >  1:1146298/1‑140 (MQ=255)                                                         cAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCggg                                                                               >  1:1026239/1‑140 (MQ=255)                                                           gCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCTAGGCAAAATGCGGGAt                                                                             >  2:1053594/1‑140 (MQ=255)                                                           gCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGAt                                                                             <  1:822031/140‑1 (MQ=255)                                                            cAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATc                                                                            <  1:980103/140‑1 (MQ=255)                                                             aaTATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCa                                                                           <  1:481583/140‑1 (MQ=255)                                                               tatGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCAcc                                                                         >  2:495898/1‑140 (MQ=255)                                                               tatGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCAcc                                                                         >  1:753825/1‑140 (MQ=255)                                                                 tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCaa                                                                                       <  2:564614/124‑1 (MQ=255)                                                                 tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCaa                                                                                       >  1:564614/1‑124 (MQ=255)                                                                 tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGt                                                                       <  2:753825/140‑1 (MQ=255)                                                                 tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGt                                                                       >  1:619665/1‑140 (MQ=255)                                                                 tGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGt                                                                       >  2:1100552/1‑140 (MQ=255)                                                                    tGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCg                                                                                 <  2:144436/127‑1 (MQ=255)                                                                    tGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCg                                                                                 >  1:144436/1‑127 (MQ=255)                                                                      aaGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCAcc                                                                         >  1:870004/1‑133 (MQ=255)                                                                      aaGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCAcc                                                                         <  2:870004/133‑1 (MQ=255)                                                                      aaGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGaa                                                                  >  2:741214/1‑140 (MQ=255)                                                                      aaGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGaa                                                                  >  1:344237/1‑140 (MQ=255)                                                                       aGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGaaa                                                                 <  2:1107436/140‑1 (MQ=255)                                                                           tCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCAcc                                                                         >  1:1112896/1‑128 (MQ=255)                                                                           tCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCAcc                                                                         <  2:1112896/128‑1 (MQ=255)                                                                           tCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGt                                                             >  1:27569/1‑140 (MQ=255)                                                                           tCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGt                                                             >  2:1052758/1‑140 (MQ=255)                                                                            cGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTc                                                            >  1:843274/1‑140 (MQ=255)                                                                            cGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTc                                                            >  1:1001272/1‑140 (MQ=255)                                                                                gCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCcgcg                                                        >  1:174950/1‑140 (MQ=255)                                                                                gCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCcgcg                                                        <  1:105206/140‑1 (MQ=255)                                                                                  ggTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCt                                                      >  2:19729/1‑140 (MQ=255)                                                                                   gTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTc                                                     >  2:1064602/1‑140 (MQ=255)                                                                                     ataACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCa                                                   <  1:741214/140‑1 (MQ=255)                                                                                      taACGATGATTTCTGATTGAATAAAGATATTTCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCAc                                                  >  2:1145244/1‑140 (MQ=255)                                                                                         cGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGgc                                               >  1:747519/1‑140 (MQ=255)                                                                                          gatgatTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGgcg                                              <  1:635954/140‑1 (MQ=255)                                                                                             gatTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTg                                           >  1:179609/1‑140 (MQ=255)                                                                                              atTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGa                                          >  2:77874/1‑140 (MQ=255)                                                                                                ttCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGAtt                                        >  2:1173846/1‑140 (MQ=255)                                                                                                       tGAATAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCa                                 <  1:264143/140‑1 (MQ=255)                                                                                                          aTAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATaa                              <  2:107857/140‑1 (MQ=255)                                                                                                          aTAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATaa                              <  1:1064602/140‑1 (MQ=255)                                                                                                          aTAAAGATATTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATaa                              <  2:27569/140‑1 (MQ=255)                                                                                                                atatTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGt                        <  2:474703/140‑1 (MQ=255)                                                                                                                 tatTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTa                       <  1:391265/140‑1 (MQ=255)                                                                                                                  atTCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAg                      <  2:619665/140‑1 (MQ=255)                                                                                                                   ttCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAgg                     >  1:1015749/1‑140 (MQ=255)                                                                                                                   ttCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAgg                     <  2:1015749/140‑1 (MQ=255)                                                                                                                    tCCTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAggg                    <  2:843274/140‑1 (MQ=255)                                                                                                                     ccTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCCGGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAggg                    >  2:118123/1‑140 (MQ=255)                                                                                                                      cTGCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGAc                  <  1:1173846/140‑1 (MQ=255)                                                                                                                        gCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAg                      >  1:1136652/1‑134 (MQ=255)                                                                                                                        gCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAg                      <  2:1136652/134‑1 (MQ=255)                                                                                                                        gCATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGATGa                <  2:1026239/140‑1 (MQ=255)                                                                                                                         cATATACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGat               >  1:400985/1‑140 (MQ=255)                                                                                                                          atatACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGata              >  1:538333/1‑140 (MQ=255)                                                                                                                          atatACATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGata              >  2:725780/1‑140 (MQ=255)                                                                                                                             taCATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGataaat           <  2:1001272/140‑1 (MQ=255)                                                                                                                             taCATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGataaat           <  2:538333/140‑1 (MQ=255)                                                                                                                             taCATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGataaat           >  2:320737/1‑140 (MQ=255)                                                                                                                              aCATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGataaata          <  2:157149/140‑1 (MQ=255)                                                                                                                              aCATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGataaata          <  1:1052758/140‑1 (MQ=255)                                                                                                                               cATCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGataaataa         <  2:359970/140‑1 (MQ=255)                                                                                                                                aTCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAaataat        >  2:188028/1‑140 (MQ=255)                                                                                                                                aTCGGCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAaataat        >  2:429526/1‑140 (MQ=255)                                                                                                                                   ggCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGt     >  1:531346/1‑140 (MQ=255)                                                                                                                                   ggCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGt     <  1:1145244/140‑1 (MQ=255)                                                                                                                                   ggCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGt     >  1:391843/1‑140 (MQ=255)                                                                                                                                   ggCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGt     <  2:800279/140‑1 (MQ=255)                                                                                                                                   ggCCTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGt     <  2:52501/140‑1 (MQ=255)                                                                                                                                     ccTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAggg                    >  1:323568/1‑123 (MQ=255)                                                                                                                                     ccTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAggg                    <  2:323568/123‑1 (MQ=255)                                                                                                                                     ccTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGa                >  1:739169/1‑127 (MQ=255)                                                                                                                                     ccTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGa                <  2:739169/127‑1 (MQ=255)                                                                                                                                      cTGCGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTgg  <  2:179609/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          TAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCATCAAAATATTTCAGCGTTGCC‑GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGG  >  NC_000913/3066247‑3066519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑