Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,097,958	C→T	A231V (GCG→GTG)	yggW →	putative coproporphyrinogen‑III oxidase‑like protein YggW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,097,958	0	C	T	100.0%	244.9 / NA	69	A231V (GCG→GTG)	yggW	putative coproporphyrinogen‑III oxidase‑like protein YggW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/38);  total (31/38)

CTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGG  >  NC_000913/3097822‑3098094                                                                                                                                         |                                                                                                                                         cTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTggg                                                                                                                                       <  1:382310/140‑1 (MQ=255)    aaTCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTa                                                                                                                                    >  1:917562/1‑140 (MQ=255)      tCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATc                                                                                                                                  <  1:811660/140‑1 (MQ=255)      tCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATc                                                                                                                                  >  1:897830/1‑140 (MQ=255)           cgcATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCaa                                                                                                                             <  1:1087227/140‑1 (MQ=255)                          aTCAACTGACCATCGAACCCAATTCGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCt                                                                                                              <  2:321378/140‑1 (MQ=255)                          aTCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCt                                                                                                              <  2:43516/140‑1 (MQ=255)                          aTCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCt                                                                                                              >  1:43516/1‑140 (MQ=255)                          aTCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCt                                                                                                              <  2:147102/140‑1 (MQ=255)                          aTCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCt                                                                                                              <  2:1121073/140‑1 (MQ=255)                           tCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCtt                                                                                                             >  2:252774/1‑140 (MQ=255)                           tCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCtt                                                                                                             <  1:822630/140‑1 (MQ=255)                            cAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTa                                                                                                            >  1:1167184/1‑140 (MQ=255)                                 gACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCa                                                                                                       <  2:643180/140‑1 (MQ=255)                                  aCCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCaa                                                                                                      >  1:187504/1‑140 (MQ=255)                                  aCCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCaa                                                                                                      >  2:887888/1‑140 (MQ=255)                                    cATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAAc                                                                                                    <  1:852198/140‑1 (MQ=255)                                    cATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGAACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAAc                                                                                                    <  1:356882/140‑1 (MQ=255)                                            ccAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTAt                                                                                            <  2:917562/140‑1 (MQ=255)                                             cAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATc                                                                                           <  1:252774/140‑1 (MQ=255)                                                      gTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATc                                                                                           >  2:187039/1‑131 (MQ=255)                                                      gTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATc                                                                                           <  1:187039/131‑1 (MQ=255)                                                      gTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGc                                                                                  >  2:22287/1‑140 (MQ=255)                                                       tttGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGca                                                                                 <  1:131476/140‑1 (MQ=255)                                                       tttGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGca                                                                                 <  1:716939/140‑1 (MQ=255)                                                         tGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGcaca                                                                               >  1:1033165/1‑140 (MQ=255)                                                            ttCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAAtc                                                                            <  2:1167184/140‑1 (MQ=255)                                                                         gTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGgcg                                                               <  1:126135/140‑1 (MQ=255)                                                                           gctgcCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCt                                                             <  1:887888/140‑1 (MQ=255)                                                                           gctgcCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCt                                                             <  1:311279/140‑1 (MQ=255)                                                                              gcCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTg                                                          <  2:187504/140‑1 (MQ=255)                                                                                cGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGt                                                        <  2:420061/140‑1 (MQ=255)                                                                                   aCGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGt                                                        <  2:1018587/137‑1 (MQ=255)                                                                                   aCGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGt                                                        >  1:1018587/1‑137 (MQ=255)                                                                                   aCGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGAc                                                     <  1:1138901/140‑1 (MQ=255)                                                                                   aCGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGAc                                                     <  2:868511/140‑1 (MQ=255)                                                                                    cGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACt                                                    >  1:292586/1‑140 (MQ=255)                                                                                     gATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTa                                                   <  1:22287/140‑1 (MQ=255)                                                                                       tGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTa                                                   >  2:255042/1‑138 (MQ=255)                                                                                       tGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTa                                                   <  1:255042/138‑1 (MQ=255)                                                                                       tGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACa                                                 <  2:685444/140‑1 (MQ=255)                                                                                                   ggATATATTCGAACATGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCt                                     <  2:1140561/140‑1 (MQ=255)                                                                                                         atTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTgcggcg                               >  2:404792/1‑140 (MQ=255)                                                                                                         atTCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTgcggcg                               >  2:925982/1‑140 (MQ=255)                                                                                                           tCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTgcggc                                <  1:251164/137‑1 (MQ=255)                                                                                                           tCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTgcggc                                >  2:251164/1‑137 (MQ=255)                                                                                                           tCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGca                             <  1:300123/140‑1 (MQ=255)                                                                                                           tCGAACAGGGGCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGca                             >  1:46557/1‑140 (MQ=255)                                                                                                                  ggggCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCa                      >  2:1040225/1‑140 (MQ=255)                                                                                                                   gggCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCGAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCaa                     >  2:798537/1‑140 (MQ=255)                                                                                                                   gggCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCaa                     >  1:824808/1‑140 (MQ=255)                                                                                                                   gggCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCaa                     >  1:575146/1‑140 (MQ=255)                                                                                                                   gggCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCaa                     >  1:1142658/1‑140 (MQ=255)                                                                                                                   gggCATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCaa                     >  2:749210/1‑140 (MQ=255)                                                                                                                      cATCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGt                  <  1:749210/140‑1 (MQ=255)                                                                                                                       aTCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCGAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTg                 <  1:798537/140‑1 (MQ=255)                                                                                                                        tCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGt                  <  1:641650/138‑1 (MQ=255)                                                                                                                        tCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGt                  <  1:1107567/138‑1 (MQ=255)                                                                                                                        tCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGt                  >  2:641650/1‑138 (MQ=255)                                                                                                                        tCAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGt                  >  2:1107567/1‑138 (MQ=255)                                                                                                                         cAGTTATTAACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGAc               <  2:292586/140‑1 (MQ=255)                                                                                                                              attaACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGAc               >  1:798738/1‑135 (MQ=255)                                                                                                                              attaACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGAc               <  2:798738/135‑1 (MQ=255)                                                                                                                               ttaACCGCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTccc         >  2:645650/1‑140 (MQ=255)                                                                                                                                     gCAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATgg   >  1:1151682/1‑140 (MQ=255)                                                                                                                                      cAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGa      >  2:756417/1‑136 (MQ=255)                                                                                                                                      cAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGa      <  1:756417/136‑1 (MQ=255)                                                                                                                                      cAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATggg  >  2:63912/1‑140 (MQ=255)                                                                                                                                      cAGTGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTAGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATggg  >  1:284521/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGG  >  NC_000913/3097822‑3098094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑