Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,158,512	G→A	R288H (CGC→CAC)	yqhG →	DUF3828 domain‑containing protein YqhG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,158,512	0	G	A	100.0%	166.2 / NA	49	R288H (CGC→CAC)	yqhG	DUF3828 domain‑containing protein YqhG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/31);  total (18/31)

AGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTT  >  NC_000913/3158384‑3158639                                                                                                                                 |                                                                                                                                aGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAg                                                                                                                        <  2:669895/138‑1 (MQ=255) aGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAg                                                                                                                        >  1:669895/1‑138 (MQ=255) aGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCg                                                                                                                      >  2:224739/1‑140 (MQ=255)  gTCGTGGAAAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCgg                                                                                                                     <  2:132812/140‑1 (MQ=255)  gTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCgg                                                                                                                     <  2:763251/140‑1 (MQ=255)              aCCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTaa                                                                                                         <  2:1034932/140‑1 (MQ=255)                  ccgATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAg                                                                                                     >  2:102387/1‑140 (MQ=255)                  ccgATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAg                                                                                                     >  2:118283/1‑140 (MQ=255)                   cgATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGa                                                                                                    <  2:456178/140‑1 (MQ=255)                                aTTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCT‑CTTAAGCAGATTGAGGCAAAAACt                                                                                      >  2:197541/1‑140 (MQ=255)                                      gTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTgccgc                                                                                 <  1:224739/140‑1 (MQ=255)                                       ttCGTTTTACGATGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTgccgcc                                                                                <  2:1034896/140‑1 (MQ=255)                                          gTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGa                                                                             <  1:657966/140‑1 (MQ=255)                                          gTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGa                                                                             <  2:838026/140‑1 (MQ=255)                                            tttACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGAtt                                                                           >  1:15824/1‑140 (MQ=255)                                             ttACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTa                                                                          <  2:46583/140‑1 (MQ=255)                                                  cTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCa                                                                     <  2:805855/140‑1 (MQ=255)                                                       cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  1:102387/140‑1 (MQ=255)                                                       cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  2:245470/140‑1 (MQ=255)                                                       cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  2:541842/140‑1 (MQ=255)                                                       cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  2:619640/140‑1 (MQ=255)                                                       cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  2:790527/140‑1 (MQ=255)                                                       cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  1:979149/140‑1 (MQ=255)                                                       cAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAg                                                                <  1:295088/140‑1 (MQ=255)                                                          tGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTg                                                             >  1:1014687/1‑140 (MQ=255)                                                          tGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTg                                                             >  2:655668/1‑140 (MQ=255)                                                            gCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGaa                                                           <  2:15824/140‑1 (MQ=255)                                                               acaacGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTa                                                        >  2:658821/1‑140 (MQ=255)                                                                  acGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCATATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAAt                                                     <  1:12168/140‑1 (MQ=255)                                                                  acGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAAt                                                     <  1:196139/140‑1 (MQ=255)                                                                   cGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATa                                                    >  2:93355/1‑140 (MQ=255)                                                                          cAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTa                                             >  2:820174/1‑140 (MQ=255)                                                                            aGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGc                                           <  1:1168134/140‑1 (MQ=255)                                                                               aCTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCgg                                        >  1:1081565/1‑140 (MQ=255)                                                                                 tGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGaa                                      <  2:798111/140‑1 (MQ=255)                                                                                   gTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACa                                    >  1:1047027/1‑140 (MQ=255)                                                                                        ataGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACaa                                                >  2:924332/3‑123 (MQ=255)                                                                                        ataGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACaa                                                <  1:924332/121‑1 (MQ=255)                                                                                        aCAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACaataaa                               >  2:494896/1‑140 (MQ=255)                                                                                             ggcgCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCgg                                        >  1:1074510/1‑126 (MQ=255)                                                                                             ggcgCGAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCgg                                        <  2:1074510/126‑1 (MQ=255)                                                                                                gcgAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAggg                       <  2:893549/140‑1 (MQ=255)                                                                                                  gAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                     <  2:460913/140‑1 (MQ=255)                                                                                                  gAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                     <  1:93355/140‑1 (MQ=255)                                                                                                  gAAGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                     <  1:820174/140‑1 (MQ=255)                                                                                                  gAAGGCAAGTGCGAAATCGCTGATTTTATCCACGCGAACAGCGGCAGCCTACTTGAGCAGATTGAGGCAAATACTGACGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGaa                     <  2:775919/140‑1 (MQ=255)                                                                                                    aGGCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAaca                   <  2:1014687/140‑1 (MQ=255)                                                                                                      gCAAGTGGGAAATCGCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACt                 <  1:658821/140‑1 (MQ=255)                                                                                                                    gCTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGAtt   >  1:747596/1‑140 (MQ=255)                                                                                                                     cTGATTTTATCCACCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGAttt  >  1:590565/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                AGTCCTGGAGAAAACCGCCGATTCCGCCCGCATTCATGTTCGTTTTACGCTGACCAATGGCAACAACGAAGAGCAAGAACTGGTTTTACAGCGGCGCGAAGGCAAGTGGGAAATCGCTGATTTTATCCGCCCGAACAGCGGCAGCCTACTTAAGCAGATTGAGGCAAAAACTGCCGCCAGATTAAAGCAATGAGCTGAATTAAATAACAATTAGCCGGAACAATAAATAAAAGGGAACACTATATGAAAACGATTT  >  NC_000913/3158384‑3158639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑