Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,233,830	A→G	S105S (TCT→TCC)	higA ←	antitoxin/DNA‑binding transcriptional repressor HigA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,233,830	0	A	G	100.0%	259.5 / NA	70	S105S (TCT→TCC)	higA	antitoxin/DNA‑binding transcriptional repressor HigA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/40);  total (30/40)

GCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCATTAAATTCCGCAAATTCGGGCGCTGATTCT  >  NC_000913/3233697‑3233962                                                                                                                                       |                                                                                                                                     gcgcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTa                                                                                                                                >  2:665527/1‑140 (MQ=255)     cGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGc                                                                                                                            >  2:1055948/1‑140 (MQ=255)     cGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGc                                                                                                                            >  2:911927/1‑140 (MQ=255)              cGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCg                                                                                                                   >  2:1067704/1‑140 (MQ=255)              cGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCg                                                                                                                   <  1:841771/140‑1 (MQ=255)               gCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCgg                                                                                                                  <  2:1074207/140‑1 (MQ=255)                   ccGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGa                                                                                                              <  2:95924/140‑1 (MQ=255)                    cGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGAt                                                                                                             <  2:917341/140‑1 (MQ=255)                        aCGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGa                                                                                                         <  2:686517/140‑1 (MQ=255)                        aCGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGa                                                                                                         <  2:1114478/140‑1 (MQ=255)                        aCGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGa                                                                                                         <  1:293311/140‑1 (MQ=255)                         cGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGaa                                                                                                        <  1:27983/140‑1 (MQ=255)                           tCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAg                                                                                                      >  2:1157444/1‑140 (MQ=255)                            cgcgTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAgg                                                                                                     >  1:326216/1‑140 (MQ=255)                             gcgTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAggg                                                                                                    <  1:54878/140‑1 (MQ=255)                               gTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGa                                                                                                         <  1:1066771/133‑1 (MQ=255)                               gTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGtt                                                                                                  <  2:722894/140‑1 (MQ=255)                               gTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGtt                                                                                                  <  1:1067704/140‑1 (MQ=255)                               gTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTGAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGa                                                                                                         >  2:1066771/1‑133 (MQ=255)                                ttAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTa                                                                                                 <  1:1025718/140‑1 (MQ=255)                                  aaTCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTaaa                                                                                               <  1:580153/140‑1 (MQ=255)                                       aTAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata                                                                                          <  2:1018166/140‑1 (MQ=255)                                        tAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCatat                                                                                         <  1:914111/140‑1 (MQ=255)                                        tAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCatat                                                                                         <  1:209916/140‑1 (MQ=255)                                          aaCAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTGCTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTg                                                                                       <  2:1133207/140‑1 (MQ=255)                                          aaCAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGCTAAACCATATTg                                                                                       <  2:206023/140‑1 (MQ=255)                                           aCAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGa                                                                                      >  1:708394/1‑140 (MQ=255)                                             aaGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATc                                                                                    >  2:699316/1‑140 (MQ=255)                                             aaGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATc                                                                                    >  2:662433/1‑140 (MQ=255)                                             aaGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATc                                                                                    >  1:728367/1‑140 (MQ=255)                                               ggCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCa                                                                                  >  2:947468/1‑140 (MQ=255)                                               ggCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCa                                                                                  >  2:519656/1‑140 (MQ=255)                                                gCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCAt                                                                                 <  2:654989/140‑1 (MQ=255)                                                gCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCAt                                                                                 <  1:911927/140‑1 (MQ=255)                                                gCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCAt                                                                                 >  1:676501/1‑140 (MQ=255)                                                gCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCAt                                                                                 <  1:1157444/140‑1 (MQ=255)                                                gCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCAt                                                                                 >  1:1159117/1‑140 (MQ=255)                                                 cGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATa                                                                                <  1:1055948/140‑1 (MQ=255)                                                  gggAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATaa                                                                               >  2:97150/1‑140 (MQ=255)                                                  gggAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATaa                                                                               >  1:453363/1‑140 (MQ=255)                                                    gagaAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCatat                                                                                         <  1:991511/128‑1 (MQ=255)                                                    gagaAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCatat                                                                                         >  2:991511/1‑128 (MQ=255)                                                         aTGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAAGGTACg                                                                        >  1:1028817/1‑140 (MQ=255)                                                          tGCCGAATCGCGTTGCCAATTTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATa                                                                                >  2:1150199/1‑132 (MQ=255)                                                          tGCCGAATCGCGTTGCCAATTTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATa                                                                                <  1:1150199/132‑1 (MQ=255)                                                            ccGACTCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAAt                                                                     <  2:1079613/140‑1 (MQ=255)                                                            ccGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAAt                                                                     <  1:947468/140‑1 (MQ=255)                                                            ccGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAAt                                                                     <  1:807285/140‑1 (MQ=255)                                                            ccGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAAt                                                                     <  1:662433/140‑1 (MQ=255)                                                            ccGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAAt                                                                     <  1:665527/140‑1 (MQ=255)                                                               aaTCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCAc                                                                  <  1:718361/140‑1 (MQ=255)                                                                          cAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGc                                                       <  1:519656/140‑1 (MQ=255)                                                                                ttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGc                                                  <  2:1159117/140‑1 (MQ=255)                                                                                ttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGc                                                  <  2:517953/140‑1 (MQ=255)                                                                                 tttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCa                                                 <  1:97150/140‑1 (MQ=255)                                                                                        tgtTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTg                                          <  1:699316/140‑1 (MQ=255)                                                                                        tgtTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTg                                          <  2:676501/140‑1 (MQ=255)                                                                                         gtTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGc                                                       >  2:470754/1‑126 (MQ=255)                                                                                         gtTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGc                                                       <  1:470754/126‑1 (MQ=255)                                                                                                    aaTTTCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCAtt                              >  1:364339/1‑140 (MQ=255)                                                                                                       ttCCTCTTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCATTaaa                           <  2:679721/140‑1 (MQ=255)                                                                                                            ctTCCCGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCATTAAATTCCg                      >  1:783764/1‑140 (MQ=255)                                                                                                               cccGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCATTAAATTCCGCaa                   >  2:31377/1‑140 (MQ=255)                                                                                                                ccGCTCAAAACGCGTGACACCATGGATTTACTGTCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCATTAAATTCCGCaaa                  >  2:397360/1‑140 (MQ=255)                                                                                                                 cGCTCAAAACGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCATTAAATTCCGCAAAt                 >  2:849469/1‑140 (MQ=255)                                                                                                                       aaaCGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTg                                                                                       <  1:1155807/64‑1 (MQ=255)                                                                                                                       aaaCGCGTGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTg                                                                                       >  2:1155807/1‑64 (MQ=255)                                                                                                                              tGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCATTAAATTCCGCAAATTCGGGCGCTGAtt    >  1:429662/1‑140 (MQ=255)                                                                                                                              tGACACCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCATTAAATTCCGCAAATTCGGGCGCTGAtt    >  1:181778/1‑140 (MQ=255)                                                                                                                                acacCATGGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGATAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGACAAGCATGGCTTGAACCATGGCATTACATTCCGCAAATTCGGGCCCTGAttct  >  2:1050452/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     GCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAA‑TTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATCCATAAGGGTACGAATCACGGCTATACCGCCAGGCATGGCTTGAGCCATGGCATTAAATTCCGCAAATTCGGGCGCTGATTCT  >  NC_000913/3233697‑3233962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑