Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,283,280	C→T	I46I (ATC→ATT)	kbaY →	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,283,280	0	C	T	100.0%	227.4 / NA	64	I46I (ATC→ATT)	kbaY	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/33);  total (31/33)

AGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGATATTC  >  NC_000913/3283146‑3283413                                                                                                                                       |                                                                                                                                      aGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGc                                                                                                                                  >  2:955323/1‑140 (MQ=255)   cATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCg                                                                                                                                <  1:241220/140‑1 (MQ=255)      tATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGaa                                                                                                                             >  2:547607/1‑140 (MQ=255)       aTCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAAc                                                                                                                            >  1:905630/1‑140 (MQ=255)             aCTAAATATCTGTTACAGGACGCCGAGGCCAATGCCTCCGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      >  2:788309/1‑140 (MQ=255)             aCTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      >  2:102265/1‑140 (MQ=255)             aCTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      <  1:50051/140‑1 (MQ=255)               tAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGa                                                                                                                    <  1:945485/140‑1 (MQ=255)               tAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGa                                                                                                                    <  2:365283/140‑1 (MQ=255)               tAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGa                                                                                                                    <  2:1054596/140‑1 (MQ=255)                  atatCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCt                                                                                                                 >  2:1117461/1‑140 (MQ=255)                      cTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTaa                                                                                                             <  1:955323/140‑1 (MQ=255)                            cAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACAt                                                                                                       <  1:499724/140‑1 (MQ=255)                               gACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      >  2:988050/1‑122 (MQ=255)                               gACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCggg                                                                                                                      <  1:988050/122‑1 (MQ=255)                                aCGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATcgcg                                                                                                   <  2:923629/140‑1 (MQ=255)                                 cGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCgcgc                                                                                                  >  1:85619/1‑140 (MQ=255)                                   cccAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTg                                                                                                >  2:404866/1‑140 (MQ=255)                                   cccAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTg                                                                                                >  2:450687/1‑140 (MQ=255)                                   cccAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTg                                                                                                >  2:58370/1‑140 (MQ=255)                                      aGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGaa                                                                                             <  1:547607/140‑1 (MQ=255)                                       ggCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGTCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAag                                                                                            >  2:284071/1‑140 (MQ=255)                                       ggCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAag                                                                                            <  1:58370/140‑1 (MQ=255)                                        gCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAaga                                                                                           <  1:1117461/140‑1 (MQ=255)                                         ccAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCACGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTAGAagag                                                                                          >  2:477529/1‑140 (MQ=255)                                             tGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCt                                                                                      >  2:558498/1‑140 (MQ=255)                                               gCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTAc                                                                                    <  1:637302/140‑1 (MQ=255)                                                        tGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTgtgt                                                                           >  2:213941/1‑140 (MQ=255)                                                         gCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTa                                                                          <  1:865638/140‑1 (MQ=255)                                                           cTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGTCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAgc                                                                        <  1:284071/140‑1 (MQ=255)                                                             gCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAgcgc                                                                      <  1:63713/140‑1 (MQ=255)                                                                     cATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCca                                                              <  2:814259/140‑1 (MQ=255)                                                                     cATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCca                                                              <  2:1052517/140‑1 (MQ=255)                                                                        tCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACaa                                                           >  2:785689/1‑140 (MQ=255)                                                                         cATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAAc                                                          >  2:180796/1‑140 (MQ=255)                                                                          aTAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAAcc                                                         <  2:352078/140‑1 (MQ=255)                                                                              cGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTaca                                                     <  1:293479/140‑1 (MQ=255)                                                                                ccGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTacaac                                                   >  2:263476/1‑140 (MQ=255)                                                                                  gagaCGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCca                                                              >  1:509342/1‑127 (MQ=255)                                                                                  gagaCGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCca                                                              <  2:509342/127‑1 (MQ=255)                                                                                   agaCGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATg                                                >  2:827059/1‑140 (MQ=255)                                                                                                gATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGc                                   <  1:558498/140‑1 (MQ=255)                                                                                                   ccTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCAtc                                >  2:203525/1‑140 (MQ=255)                                                                                                   ccTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCAtc                                >  2:906696/1‑140 (MQ=255)                                                                                                   ccTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCAtc                                >  2:1086791/1‑140 (MQ=255)                                                                                                       gAAGTGTGCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGa                            >  2:606541/1‑140 (MQ=255)                                                                                                             tgCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGac                           >  2:240231/1‑135 (MQ=255)                                                                                                             tgCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGac                           <  1:240231/135‑1 (MQ=255)                                                                                                             tgCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGAccacca                      >  2:902378/1‑140 (MQ=255)                                                                                                             tgCAGTGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGAccacca                      >  2:418812/1‑140 (MQ=255)                                                                                                                  tGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAAt                 <  1:263476/140‑1 (MQ=255)                                                                                                                  tGAAATGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAAt                 <  1:785689/140‑1 (MQ=255)                                                                                                                    aaaTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCg               <  1:477529/140‑1 (MQ=255)                                                                                                                     aaTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGa                   <  1:692506/135‑1 (MQ=255)                                                                                                                     aaTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGa                   >  2:692506/1‑135 (MQ=255)                                                                                                                     aaTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGc              >  2:421630/1‑140 (MQ=255)                                                                                                                      aTGCGATCGCCGGTGATTCTCGCCGGACCGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCt             <  1:788309/140‑1 (MQ=255)                                                                                                                      aTGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCt             <  1:102265/140‑1 (MQ=255)                                                                                                                       tGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTg            >  1:677421/1‑140 (MQ=255)                                                                                                                       tGCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTg            >  1:165780/1‑140 (MQ=255)                                                                                                                        gCGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTgg           <  1:902378/140‑1 (MQ=255)                                                                                                                         cGATCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGga          <  1:906696/140‑1 (MQ=255)                                                                                                                            tCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCg               >  2:532800/1‑132 (MQ=255)                                                                                                                            tCGCCGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCg               <  1:532800/132‑1 (MQ=255)                                                                                                                               ccGGTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGatat    <  1:418812/140‑1 (MQ=255)                                                                                                                                 ggTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGATATTc  <  2:336321/140‑1 (MQ=255)                                                                                                                                 ggTGATTCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGATATTc  >  1:532744/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      AGCATTATCTCCACTAAATATCTGTTACAGGACGCCCAGGCCAATGGCTACGCGGTGCCTGCTTTTAACATTCATAACGCCGAGACGATCCAAGCGATCCTCGAAGTGTGCAGTGAAATGCGATCGCCGGTGATCCTCGCCGGAACGCCGGGGACCTTTAAACACATCGCGCTGGAAGAGATCTACGCCCTGTGTAGCGCCTATTCCACAACCTACAACATGCCACTGGCGCTGCATCTCGACCACCACGAATCGCTGGATGATATTC  >  NC_000913/3283146‑3283413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑