Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	468,365	(A)7→6	intergenic (+135/‑18)	cof → / → decR	HMP‑PP phosphatase/DNA‑binding transcriptional activator DecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	468,359	0	A	.	100.0%	221.3 / NA	52	intergenic (+129/‑24)	cof/decR	HMP‑PP phosphatase/DNA‑binding transcriptional activator DecR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (18/34);  total (18/34)

CCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCC  >  NC_000913/468223‑468482                                                                                                                                         |                                                                                                                            ccGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGaaaa                                                                                                                          >  1:1005077/1‑140 (MQ=255) ccGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGaaaa                                                                                                                          >  1:263166/1‑140 (MQ=255)   gAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGaaaaaa                                                                                                                        <  1:62574/140‑1 (MQ=255)    aaTAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGaaaaaat                                                                                                                       >  2:673210/1‑139 (MQ=255)             aTCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTgg                                                                                                             <  2:754013/140‑1 (MQ=255)             aTCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTgg                                                                                                             <  2:363545/140‑1 (MQ=255)             aTCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTgg                                                                                                             <  1:789695/140‑1 (MQ=255)              tCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGa                                                                                                            <  1:1271631/140‑1 (MQ=255)              tCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGa                                                                                                            <  2:1005077/140‑1 (MQ=255)              tCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGa                                                                                                            >  1:856382/1‑140 (MQ=255)              tCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGa                                                                                                            <  1:635822/140‑1 (MQ=255)               cccTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTgg                                                                                                             <  2:654921/138‑1 (MQ=255)               cccTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTgg                                                                                                             >  1:654921/1‑138 (MQ=255)                  ttCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAggg                                                                                                        <  2:1320865/140‑1 (MQ=255)                  ttCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAggg                                                                                                        <  2:263166/140‑1 (MQ=255)                  ttCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAggg                                                                                                        <  2:1053926/140‑1 (MQ=255)                  ttCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAggg                                                                                                        <  2:498666/140‑1 (MQ=255)                  ttCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAggg                                                                                                        <  1:608647/140‑1 (MQ=255)                     cAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTa                                                                                                     >  1:533234/1‑140 (MQ=255)                       gCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATg                                                                                                   <  1:95740/140‑1 (MQ=255)                        cACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGt                                                                                                  >  2:430386/1‑140 (MQ=255)                             ggCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAggg                                                                                                        <  2:147640/129‑1 (MQ=255)                             ggCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAggg                                                                                                        >  1:147640/1‑129 (MQ=255)                             ggCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGAt                                                                                             >  1:575499/1‑140 (MQ=255)                             ggCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGAt                                                                                             >  2:171655/1‑140 (MQ=255)                             ggCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGAt                                                                                             >  2:561640/1‑140 (MQ=255)                              gCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATa                                                                                            <  2:532987/140‑1 (MQ=255)                                  ttGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAt                                                                                        <  1:979750/140‑1 (MQ=255)                                  ttGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAt                                                                                        <  2:565716/140‑1 (MQ=255)                                  ttGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAt                                                                                        <  1:891510/140‑1 (MQ=255)                                  ttGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAAt                                                                                        <  1:1340040/140‑1 (MQ=255)                                          ttttttttGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTa                                                                                <  2:533234/140‑1 (MQ=255)                                          ttttttttGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTa                                                                                <  1:561640/140‑1 (MQ=255)                                                 tGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAgctgct                                                                         <  2:856382/140‑1 (MQ=255)                                                 tGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAgctgct                                                                         <  1:673210/140‑1 (MQ=255)                                                    gTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGc                                                                      >  1:295332/1‑140 (MQ=255)                                                    gTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGc                                                                      >  2:1152893/1‑140 (MQ=255)                                                    gTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGc                                                                      >  1:1023877/1‑140 (MQ=255)                                                        aaTTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTa                                                                  <  2:575499/140‑1 (MQ=255)                                                              tCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTgcag                                                            <  1:767834/140‑1 (MQ=255)                                                                        ccGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCa                                                  <  1:430386/140‑1 (MQ=255)                                                                                tttAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCctctct                                          <  2:1261843/140‑1 (MQ=255)                                                                                         cacaGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCa                                 <  1:1187587/140‑1 (MQ=255)                                                                                          acaGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCAc                                >  2:622045/1‑140 (MQ=255)                                                                                                 aaaTTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGa                         >  2:1199375/1‑140 (MQ=255)                                                                                                        cGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGtt                  <  2:1123605/140‑1 (MQ=255)                                                                                                        cGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGtt                  >  1:403999/1‑140 (MQ=255)                                                                                                        cGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGtt                  >  2:604639/1‑140 (MQ=255)                                                                                                        cGTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGACACTGGCTGAAGCCGtt                  >  1:967996/1‑140 (MQ=255)                                                                                                         gTGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTa                 <  1:360569/140‑1 (MQ=255)                                                                                                          tGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTaa                >  2:204584/1‑140 (MQ=255)                                                                                                          tGATTTCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTaa                >  2:1381112/1‑140 (MQ=255)                                                                                                             tttCACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCt             <  1:1381112/140‑1 (MQ=255)                                                                                                                cACCTTTAAAATAAAATTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGAc          <  2:1215460/140‑1 (MQ=255)                                                                                                                      taaaataaaaTTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAaccac    <  2:399053/140‑1 (MQ=255)                                                                                                                        aaataaaatTAAAAGAG‑AAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCAccc  <  1:622045/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                            CCGAATAACGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCGCACAGAATAAATTGTCGTGATTTCACCTTTAAAATAAAATTAAAAGAGAAAAAAATTCTCTGTGGAAGGGCTATGTTAGATAAAATTGACCGTAAGCTGCTGGCCTTACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCC  >  NC_000913/468223‑468482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑