| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 685,941 | (C)6→5 | coding (729/741 nt) | gltJ ← | glutamate/aspartate ABC transporter membrane subunit GltJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 685,936 | 0 | C | . | 98.3% | 237.4 / ‑2.7 | 58 | coding (734/741 nt) | gltJ | glutamate/aspartate ABC transporter membrane subunit GltJ |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (24/33); minor base T (1/0); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAATCGA > NC_000913/685799‑686065 | ccccccGCCAGCATCGTCCCCCACAAAATCCCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGccc < 2:1125732/136‑1 (MQ=255) tCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGCCAAAGGAACTCCAGCCAAACTCCTACCTTTTTTGcccc > 2:213379/1‑140 (MQ=255) tCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGcccc > 1:257302/1‑140 (MQ=255) tCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGcccc > 2:71321/1‑140 (MQ=255) cccGCCAGCATCGTCCCCCACAAAATCCCAACCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGccccca < 2:1336447/139‑2 (MQ=255) cGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGcccccatg > 1:568741/1‑137 (MQ=255) ccccaaaaaaaTCCCAAACACCACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCcaggcagg < 2:446991/133‑1 (MQ=255) cccACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCg < 2:700287/140‑1 (MQ=255) caAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACt > 2:1284271/1‑140 (MQ=255) caAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACt > 2:135935/1‑140 (MQ=255) aaaaTACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACtt < 2:1357950/140‑1 (MQ=255) ccTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCa < 2:974795/139‑1 (MQ=255) aCTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCa > 2:750474/1‑140 (MQ=255) aCTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCa < 2:257302/140‑1 (MQ=255) tACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGc > 1:1048871/1‑140 (MQ=255) tACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGc > 2:767100/1‑140 (MQ=255) ccgccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAAc < 2:537908/134‑1 (MQ=255) ccgccgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAAc > 1:537908/1‑134 (MQ=255) cgTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGtcatc < 1:750474/140‑1 (MQ=255) gTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCCGGCAGGCGGACCTTACGTTCAAACAGCGtcatcc > 2:129352/1‑139 (MQ=255) gTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGtcatca < 1:213379/140‑1 (MQ=255) tGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCAc < 1:1326743/140‑1 (MQ=255) cGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAg < 1:135935/140‑1 (MQ=255) cGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAg < 2:109595/140‑1 (MQ=255) gTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCTGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCa < 2:1205445/140‑1 (MQ=255) gTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCa < 1:549831/140‑1 (MQ=255) gTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCa < 1:1149559/140‑1 (MQ=255) ttCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATaa < 1:655938/140‑1 (MQ=255) ggTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCg < 1:373308/140‑1 (MQ=255) gacagtCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCg < 1:950975/135‑1 (MQ=255) aCCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa < 1:988886/140‑1 (MQ=255) aCCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa < 2:1048871/140‑1 (MQ=255) cGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATa > 1:1384552/1‑140 (MQ=255) cGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAACGTTAATCAACACATa > 2:969015/1‑140 (MQ=255) tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATAcc > 1:360499/1‑139 (MQ=255) tCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACCCGTACATTATTT‑TCCCCCATGTTGCCAGGCAGGCGAACTTTTCGTTCAACCAGGGTCCTCACCAGCATGATAAAAACGTTAATCAacacccgtc > 2:950975/1‑135 (MQ=255) cGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGTCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa < 1:266742/130‑1 (MQ=255) cGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGTCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCa > 2:266742/1‑130 (MQ=255) cGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGc < 1:129352/140‑1 (MQ=255) gCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAg < 2:1384552/140‑1 (MQ=255) tatGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTaa < 2:568741/140‑1 (MQ=255) gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATgg > 1:195555/1‑134 (MQ=255) gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATgg < 2:195555/134‑1 (MQ=255) gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCggggg > 1:96148/1‑137 (MQ=255) gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTaa > 2:893487/1‑140 (MQ=255) gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGGaa > 1:1295928/1‑140 (MQ=255) gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGGaa > 1:53299/1‑140 (MQ=255) gaagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGGaa > 1:563527/1‑140 (MQ=255) aagggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGGaaa > 1:17453/1‑140 (MQ=255) agggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAAc < 2:1162416/140‑1 (MQ=255) agggaCAATGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAAc < 1:1284271/140‑1 (MQ=255) aaTGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTcc < 2:563527/140‑1 (MQ=255) aaTGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTcc < 1:893487/140‑1 (MQ=255) aaTGGAACTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTcc < 2:924008/140‑1 (MQ=255) aaCTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGc < 1:767100/140‑1 (MQ=255) cTCCAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGCTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAAAGACTCCCCGGCaa > 2:1388054/1‑139 (MQ=255) cAGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGCAAACGACTCCCAGGCACggg > 1:1390626/1‑140 (MQ=255) aGTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGc > 1:820628/1‑140 (MQ=255) gTCAAACTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGGATggggt > 1:1050030/1‑138 (MQ=255) aaCTCGTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGt < 1:71321/140‑1 (MQ=255) gTACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAATCg < 1:639037/140‑1 (MQ=255) tACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCAt < 2:1281843/125‑1 (MQ=255) tACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCAt > 1:1281843/1‑125 (MQ=255) tACATTATTTG‑CCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAATCGa > 1:742505/1‑140 (MQ=255) | ATCACCGCCAGCATCGTGCCCCACAAAATACCAATCACTACCGCCGTGACGGTGATTTTCAGGGTGATCACCAGCCCGTCGAGCAGATATGGCAGGGAAGGGACAATGGAACTCCAGTCAAACTCGTACATTATTTGCCCCCCATGTTGCCAGGCAGGCGAACTTTACGTTCAACCAGCGTCATCACCAGCATGATAAAAGCGTTAATCAACACATACGCCAGCGTAATGGCGGTAAACGACTCCCAGGCATGGGCTGAGTAATCGA > NC_000913/685799‑686065 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |