Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,867,926	(C)6→5	coding (828/1140 nt)	nlpD ←	murein hydrolase activator NlpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,867,921	0	C	.	100.0%	329.3 / NA	77	coding (833/1140 nt)	nlpD	murein hydrolase activator NlpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (45/32);  total (45/32)

ACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTG  >  NC_000913/2867782‑2868056                                                                                                                                            |                                                                                                                                        aCTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                                         <  2:269854/140‑1 (MQ=255)  cTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGcc                                                                                                                                        >  1:1199079/1‑140 (MQ=255)    cAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGcccc                                                                                                                                      >  1:523065/1‑140 (MQ=255)         aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGccccctcag                                                                                                                                 >  1:898796/1‑136 (MQ=255)          atcatcATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGa                                                                                                                               >  2:1382014/1‑140 (MQ=255)           tcatcaTTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGaa                                                                                                                              <  2:183705/140‑1 (MQ=255)               catTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                                         >  2:841247/1‑126 (MQ=255)               catTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                                         <  1:841247/126‑1 (MQ=255)                atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGcc                                                                                                                         <  1:798133/140‑1 (MQ=255)                 ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCa                                                                                                                        <  2:206473/140‑1 (MQ=255)                 ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCa                                                                                                                        >  2:1078561/1‑140 (MQ=255)                 ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCa                                                                                                                        >  2:280727/1‑140 (MQ=255)                 ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCa                                                                                                                        >  2:658115/1‑140 (MQ=255)                 ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCa                                                                                                                        >  2:933082/1‑140 (MQ=255)                  tATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCaa                                                                                                                       <  1:658115/140‑1 (MQ=255)                    tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAg                                                                                                                     <  1:1078561/140‑1 (MQ=255)                      tttGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGt                                                                                                                   >  2:992809/1‑140 (MQ=255)                      tttGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGt                                                                                                                   >  1:1310743/1‑140 (MQ=255)                        tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGttt                                                                                                                 >  1:1327504/1‑140 (MQ=255)                           tgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                              <  2:755358/140‑1 (MQ=255)                           tgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                              >  1:257652/1‑140 (MQ=255)                             atAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATc                                                                                                            <  1:597763/140‑1 (MQ=255)                               aaTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAATAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCAc                                                                                                          <  1:605977/140‑1 (MQ=255)                                aTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACt                                                                                                         >  2:588564/1‑140 (MQ=255)                                aTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACt                                                                                                         >  2:1194796/1‑140 (MQ=255)                                    gATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGc                                                                                                     >  1:541143/1‑140 (MQ=255)                                    gATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGACATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGc                                                                                                     >  1:789089/1‑140 (MQ=255)                                          cGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAg                                                                                               >  1:582663/1‑140 (MQ=255)                                            tAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTc                                                                                             >  2:66198/1‑140 (MQ=255)                                               ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGc                                                                                          >  1:545990/1‑140 (MQ=255)                                               ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGc                                                                                          >  1:1264166/1‑140 (MQ=255)                                               ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGc                                                                                          >  1:983850/1‑140 (MQ=255)                                                 gcgcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCAc                                                                                                          <  2:375842/122‑1 (MQ=255)                                                 gcgcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCAc                                                                                                          >  1:375842/1‑122 (MQ=255)                                                 gcgcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCa                                                                                        >  1:1194376/1‑140 (MQ=255)                                                 gcgcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCa                                                                                        >  1:259751/1‑140 (MQ=255)                                                   gcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAgc                                                                                      >  1:188685/1‑140 (MQ=255)                                                         cgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAgg                                                                                <  1:588564/140‑1 (MQ=255)                                                         cgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAgg                                                                                <  2:1199079/140‑1 (MQ=255)                                                         cgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAgg                                                                                <  2:1310743/140‑1 (MQ=255)                                                             aCCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGga                                                                            <  2:108431/140‑1 (MQ=255)                                                               cAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgaga                                                                          <  2:1194376/140‑1 (MQ=255)                                                               cAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgaga                                                                          <  2:259751/140‑1 (MQ=255)                                                                aGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGAt                                                                         <  1:66198/140‑1 (MQ=255)                                                                   aTAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGCTCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAgg                                                                      >  2:320448/1‑140 (MQ=255)                                                                    tAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGc                                                                     >  1:414480/1‑140 (MQ=255)                                                                      aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGt                                                                   >  2:687794/1‑140 (MQ=255)                                                                       acaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTa                                                                  <  2:1327504/140‑1 (MQ=255)                                                                       acaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTa                                                                  <  2:545990/140‑1 (MQ=255)                                                                       acaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTa                                                                  <  1:1211460/140‑1 (MQ=255)                                                                       acaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTa                                                                  <  2:898796/140‑1 (MQ=255)                                                                       acaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTACGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTa                                                                  >  1:764885/1‑140 (MQ=255)                                                                         aacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACt                                                                <  1:413988/140‑1 (MQ=255)                                                                         aacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACt                                                                <  1:1382014/140‑1 (MQ=255)                                                                           cgcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTgg                                                              >  1:301323/1‑140 (MQ=255)                                                                            gcgGCCATCTGCGGCCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGGTG‑CCCCCTCAGAAGCGCCAAATGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGt                                                             >  2:556760/1‑140 (MQ=255)                                                                                  aTCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGt                                                       <  1:1194796/140‑1 (MQ=255)                                                                                  aTCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGt                                                       <  2:789089/140‑1 (MQ=255)                                                                                  aTCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGt                                                       <  2:541143/140‑1 (MQ=255)                                                                                          tCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGa                                               >  2:228151/1‑140 (MQ=255)                                                                                            gcgATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACa                                             <  2:603425/140‑1 (MQ=255)                                                                                              gATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGt                                           >  1:463853/1‑140 (MQ=255)                                                                                                 aaTTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCgg                                        <  1:280727/140‑1 (MQ=255)                                                                                                   ttGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCt                                      <  2:1264166/140‑1 (MQ=255)                                                                                                    tGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTc                                     >  1:27805/1‑140 (MQ=255)                                                                                                    tGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTc                                     >  1:1385986/1‑140 (MQ=255)                                                                                                        tGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGtt                                 >  2:1193633/1‑140 (MQ=255)                                                                                                          tCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTtgt                               >  1:983460/1‑140 (MQ=255)                                                                                                          tCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTtgt                               >  1:143262/1‑140 (MQ=255)                                                                                                           ccTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTtgtg                              >  1:250355/1‑140 (MQ=255)                                                                                                                gctgcCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgctt                           >  1:1115544/1‑138 (MQ=255)                                                                                                                gctgcCTGCGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgctt                           <  2:1115544/138‑1 (MQ=255)                                                                                                                    cctgcGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGtt                                 <  1:894711/128‑1 (MQ=255)                                                                                                                    cctgcGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGtt                                 >  2:894711/1‑128 (MQ=255)                                                                                                                    cctgcGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGtt                     >  2:940600/1‑140 (MQ=255)                                                                                                                    cctgcGATATCAATCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGtt                     >  1:574278/1‑140 (MQ=255)                                                                                                                                aTCCCCTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACa         <  1:933082/140‑1 (MQ=255)                                                                                                                                     cTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTa                 >  2:604574/1‑127 (MQ=255)                                                                                                                                     cTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTa                 <  1:604574/127‑1 (MQ=255)                                                                                                                                     cTTGTTG‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGgcgc    >  2:750521/1‑140 (MQ=255)                                                                                                                                      ttgttg‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCt   >  1:303716/1‑140 (MQ=255)                                                                                                                                       tgttg‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCtg  >  1:347494/1‑140 (MQ=255)                                                                                                                                       tgttg‑CCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCtg  >  2:268803/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                        ACTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCGTTACAGGCGCTG  >  NC_000913/2867782‑2868056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑