Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,216,481	(G)6→7	coding (11/765 nt)	nfeF ←	NADPH‑dependent ferric chelate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,216,475	1	.	G	100.0%	262.4 / NA	72	R6P (CGC→CCC)	nfeF	NADPH‑dependent ferric chelate reductase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (24/48);  total (24/48)

ATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑GGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAA  >  NC_000913/3216336‑3216609                                                                                                                                             |                                                                                                                                       aTCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGc                                                                                                                                         >  1:998356/1‑140 (MQ=255)      cAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑ggggg                                                                                                                                   <  2:1139792/140‑1 (MQ=255)       aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑gggggg                                                                                                                                  <  1:527687/140‑1 (MQ=255)       aaaGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑gggggg                                                                                                                                  <  1:1163626/140‑1 (MQ=255)         aGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑gggggggt                                                                                                                                >  2:313125/1‑138 (MQ=255)         aGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑gggggggt                                                                                                                                <  2:98298/140‑3 (MQ=255)         aGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑gggggggt                                                                                                                                <  1:1239859/140‑3 (MQ=255)          gCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑gggggggtg                                                                                                                               <  1:39064/140‑4 (MQ=255)          gCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑gggggggtg                                                                                                                               <  1:707613/140‑4 (MQ=255)          gCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑gggggggtg                                                                                                                               <  2:10669/140‑4 (MQ=255)              cgcgATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTAt                                                                                                                            >  2:316992/1‑140 (MQ=255)              cgcgATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTAt                                                                                                                            >  2:1177297/1‑140 (MQ=255)               gcgATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTAtt                                                                                                                           <  1:1177297/140‑1 (MQ=255)                 gATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCa                                                                                                                         <  1:512191/140‑1 (MQ=255)                     tAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGATCGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGt                                                                                                                     >  2:964445/1‑140 (MQ=255)                     tAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGt                                                                                                                     >  1:1146711/1‑140 (MQ=255)                        aaCCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTAt                                                                                                                  <  1:716868/140‑1 (MQ=255)                         aCCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATc                                                                                                                 >  2:241721/1‑140 (MQ=255)                             tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGcc                                                                                                              <  1:537785/139‑1 (MQ=255)                             tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGcc                                                                                                              >  2:537785/1‑139 (MQ=255)                             tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCt                                                                                                             >  2:896353/1‑140 (MQ=255)                             tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCt                                                                                                             >  2:257673/1‑140 (MQ=255)                             tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCt                                                                                                             >  2:661035/1‑140 (MQ=255)                             tCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCt                                                                                                             >  1:851712/1‑140 (MQ=255)                                aGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCg                                                                                                          <  2:1105911/140‑1 (MQ=255)                                  cgcCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTg                                                                                                        <  1:1371503/140‑1 (MQ=255)                                    ccTCGCCGCCGAGGTCAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGAt                                                                                                      <  2:492619/140‑1 (MQ=255)                                    ccTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGAt                                                                                                      <  1:391837/140‑1 (MQ=255)                                        gccgccGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTa                                                                                                  <  2:1146711/140‑1 (MQ=255)                                         ccgccgAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTaa                                                                                                 <  2:180197/140‑1 (MQ=255)                                          cgccgAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt                                                                                                <  2:357244/140‑1 (MQ=255)                                          cgccgAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAAt                                                                                                <  1:50796/140‑1 (MQ=255)                                              gAGGGCAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGa                                                                                            <  1:1070779/140‑1 (MQ=255)                                              gAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGa                                                                                            <  2:12767/140‑1 (MQ=255)                                              gAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGa                                                                                            <  1:450639/140‑1 (MQ=255)                                                 gACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACACGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGatat                                                                                         >  2:319835/1‑140 (MQ=255)                                                  aCAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTaa                                                                                                 >  2:1240467/1‑131 (MQ=255)                                                  aCAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTaa                                                                                                 <  1:1240467/131‑1 (MQ=255)                                                   cAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGatatat                                                                                       <  2:885445/140‑1 (MQ=255)                                                   cAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGatatat                                                                                       <  2:851712/140‑1 (MQ=255)                                                   cAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGatatat                                                                                       <  1:241721/140‑1 (MQ=255)                                                          cTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCtaaata                                                                                <  1:93730/140‑1 (MQ=255)                                                           tGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCtaaataa                                                                               <  1:313125/140‑1 (MQ=255)                                                           tGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCtaaataa                                                                               >  2:571884/1‑140 (MQ=255)                                                           tGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCtaaataa                                                                               >  2:1113016/1‑140 (MQ=255)                                                             aaaaCCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATaaaa                                                                             <  1:257673/140‑1 (MQ=255)                                                                  cGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGc                                                                        <  2:305471/140‑1 (MQ=255)                                                                   ggCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCa                                                                       <  1:1386092/140‑1 (MQ=255)                                                                   ggCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCa                                                                       <  2:1334068/140‑1 (MQ=255)                                                                   ggCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCa                                                                       <  1:1196573/140‑1 (MQ=255)                                                                       cTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCa                                                                       >  2:314048/1‑136 (MQ=255)                                                                       cTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCa                                                                       <  1:314048/136‑1 (MQ=255)                                                                       cTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAaatg                                                                   <  1:751330/140‑1 (MQ=255)                                                                       cTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAaatg                                                                   <  1:266869/140‑1 (MQ=255)                                                                        tGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAaatga                                                                  <  2:1391490/140‑1 (MQ=255)                                                                          aTGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAaatgata                                                                <  1:964445/140‑1 (MQ=255)                                                                            gcgcTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAaatgataat                                                              <  1:561914/140‑1 (MQ=255)                                                                                 cAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTg                                                         <  2:122070/140‑1 (MQ=255)                                                                                 cAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTg                                                         <  2:658441/140‑1 (MQ=255)                                                                                    cgcgTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTa                                                      >  1:208802/1‑140 (MQ=255)                                                                                      cgTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAAt                                                    <  1:280504/140‑1 (MQ=255)                                                                                                tcagtTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAAt                                          >  1:830809/1‑140 (MQ=255)                                                                                                  agtTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGc                                        >  2:207018/1‑140 (MQ=255)                                                                                                   gtTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCa                                       <  2:354970/140‑1 (MQ=255)                                                                                                    ttCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCaa                                      >  1:1341502/1‑140 (MQ=255)                                                                                                       aCGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCg                                   <  1:1113016/140‑1 (MQ=255)                                                                                                              cgcAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa                            >  1:664593/1‑140 (MQ=255)                                                                                                              cgcAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTa                            >  1:5411/1‑140 (MQ=255)                                                                                                               gcAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAg                           <  1:640924/140‑1 (MQ=255)                                                                                                               gcAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAg                           <  1:571884/140‑1 (MQ=255)                                                                                                                cAGATCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGa                          <  2:998356/140‑1 (MQ=255)                                                                                                                   aTCATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACt                       <  1:896353/140‑1 (MQ=255)                                                                                                                     cATTGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGa                     <  1:736247/140‑1 (MQ=255)                                                                                                                       ttGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGata                   <  1:319835/140‑1 (MQ=255)                                                                                                                       ttGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGata                   <  1:316992/140‑1 (MQ=255)                                                                                                                       ttGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGata                   >  1:1210031/1‑140 (MQ=255)                                                                                                                        tGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACt                       >  2:213846/1‑135 (MQ=255)                                                                                                                        tGCGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACt                       <  1:213846/135‑1 (MQ=255)                                                                                                                          cGAACGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGt                >  2:870714/1‑140 (MQ=255)                                                                                                                            aaCGCGCTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCt              <  2:297655/140‑1 (MQ=255)                                                                                                                                 gcTGCGGGTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGt         <  2:664593/140‑1 (MQ=255)                                                                                                                                        gTAGCGGGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATaa  <  1:870714/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                       ATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAACGCGCTGCGGGTAGC‑GGGGGGTGTTATTCATTGTTATCGCCTTCGTGATGGTAATCAGATATATCTAAATAAAACTCGCAAATGATAATGATTGTTAATCATGATAAATGCAAGCGATTTGTAGAACTGATATGTCTATAGTCTGATAA  >  NC_000913/3216336‑3216609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑