Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,982,684 (C)8→9 noncoding (79/87 nt) leuT → tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,982,6761.C94.0% 288.5 / 9.0 84noncoding (71/87 nt)leuTtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (1/4);  new base C (41/38);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982538‑3982809
                                                                                                                                            |                                                                                                                                       
ggATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑c                                                                                                                                       <  2:1071973/140‑1 (MQ=255)
 gATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑cc                                                                                                                                      >  1:781342/1‑140 (MQ=255)
   ttGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑cccc                                                                                                                                    <  2:148421/140‑1 (MQ=255)
     gtgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑cccccc                                                                                                                                  <  1:308325/140‑1 (MQ=255)
      tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccccccc                                                                                                                                 >  1:967113/1‑140 (MQ=255)
      tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccccccc                                                                                                                                 >  2:1134210/1‑140 (MQ=255)
         ttCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑ccccccccct                                                                                                                              <  1:1134210/140‑3 (MQ=255)
              gTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGc‑ac                                                                                                                         >  1:364885/1‑140 (MQ=255)
                 gTCGTGGGTTCGAATCCCATTGGCCCCCCCTTTATTAGAAGTTGTGCCAATGGGAGGGTGGCGGATTTGGAAGCCGCGTTGGCTTAGGGTGTTGGGGTCCTTCCGGAGGGGGGGGTTAAGGCC‑‑CCCCCCCCTCGc‑accac                                                                                                                      <  2:1083735/140‑1 (MQ=255)
                  tCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCCCCCCCTCGC‑ACCACGa                                                                                                                    <  2:17396/140‑1 (MQ=255)
                      gggTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACtt                                                                                                                 >  2:370472/1‑140 (MQ=255)
                       ggTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACttt                                                                                                                >  2:768244/1‑140 (MQ=255)
                            gAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGTTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGa                                                                                                           >  1:630880/1‑140 (MQ=255)
                                 ccATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGa                                                                                                      <  1:862328/140‑1 (MQ=255)
                                  cATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGaa                                                                                                     >  2:625244/1‑140 (MQ=255)
                                       gCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTaaa                                                                                                >  2:514422/1‑140 (MQ=255)
                                       gCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTaaa                                                                                                >  2:311934/1‑140 (MQ=255)
                                        ccACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTaaaa                                                                                               >  2:1378207/1‑140 (MQ=255)
                                             ccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCa                                                                                          <  2:9220/140‑1 (MQ=255)
                                               attattAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCaaa                                                                                        >  2:30646/1‑140 (MQ=255)
                                                 tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                                                                      >  1:134557/1‑140 (MQ=255)
                                                  attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     <  1:370472/140‑1 (MQ=255)
                                                  attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     <  1:1378207/140‑1 (MQ=255)
                                                      gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                                                                 >  1:1189732/1‑140 (MQ=255)
                                                          ttGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                                                             >  2:212745/1‑140 (MQ=255)
                                                          ttGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                            >  2:862723/1‑140 (MQ=255)
                                                           tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                                                                <  2:223280/136‑1 (MQ=255)
                                                           tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                                                                >  1:223280/1‑136 (MQ=255)
                                                           tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                            >  1:307932/1‑140 (MQ=255)
                                                           tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                            >  1:1154584/1‑140 (MQ=255)
                                                            gtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                                                                <  2:45146/135‑1 (MQ=255)
                                                            gtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                                                                >  1:45146/1‑135 (MQ=255)
                                                             tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     <  2:908966/129‑1 (MQ=255)
                                                             tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     >  1:908966/1‑129 (MQ=255)
                                                             tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCGGTATTTCg                                                                           >  2:1174432/1‑139 (MQ=255)
                                                             tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCGGTATTTCg                                                                           <  1:1174432/139‑1 (MQ=255)
                                                             tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                                                                          >  2:571851/1‑140 (MQ=255)
                                                             tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                                                                          >  1:752877/1‑140 (MQ=255)
                                                                 aaTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                                                                      <  1:1022001/140‑1 (MQ=255)
                                                                 aaTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                                                                      >  1:290668/1‑140 (MQ=255)
                                                                   tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                                                                    >  2:307016/1‑140 (MQ=255)
                                                                   tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                                                                   <  1:862723/140‑1 (MQ=255)
                                                                    gCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                                                                   <  2:752877/140‑1 (MQ=255)
                                                                      gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcg                                                                 <  1:817470/140‑1 (MQ=255)
                                                                       aaGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcgc                                                                >  2:1370032/1‑140 (MQ=255)
                                                                              cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTGCGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTgg                                                         >  1:624473/1‑140 (MQ=255)
                                                                              cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTgg                                                         >  1:580081/1‑140 (MQ=255)
                                                                               ggAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGt                                                        <  1:613503/140‑1 (MQ=255)
                                                                                 aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg                                                      <  1:307016/140‑1 (MQ=255)
                                                                                      gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAATAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa                                                 <  2:967113/140‑1 (MQ=255)
                                                                                      gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa                                                 <  1:1370032/140‑1 (MQ=255)
                                                                                      gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa                                                 <  2:1154584/140‑1 (MQ=255)
                                                                                       tAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAAc                                                <  2:899066/140‑1 (MQ=255)
                                                                                          aCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTgg                                             >  1:1267350/1‑140 (MQ=255)
                                                                                           cgcgCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGt                                            >  1:770764/1‑140 (MQ=255)
                                                                                            gcgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGtt                                           <  1:768244/140‑1 (MQ=255)
                                                                                               cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTgg                                        <  2:781342/140‑1 (MQ=255)
                                                                                                tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTggg                                       <  2:134557/140‑1 (MQ=255)
                                                                                                       aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTg                                <  1:311934/140‑1 (MQ=255)
                                                                                                       aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTg                                <  1:514422/140‑1 (MQ=255)
                                                                                                           gtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTc                            <  1:571851/140‑1 (MQ=255)
                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  1:532945/1‑140 (MQ=255)
                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           <  2:570332/140‑1 (MQ=255)
                                                                                                            ttAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  2:605340/1‑140 (MQ=255)
                                                                                                             tAGTGTCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCgg                          <  2:630880/140‑1 (MQ=255)
                                                                                                                  tCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     >  1:1021601/1‑140 (MQ=255)
                                                                                                                  tCCTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     >  1:563521/1‑140 (MQ=255)
                                                                                                                  tCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTc                    <  2:1336988/140‑1 (MQ=255)
                                                                                                                    cTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCg                   >  1:725207/1‑140 (MQ=255)
                                                                                                                    cTTACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCg                   >  2:301693/1‑140 (MQ=255)
                                                                                                                      tACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 <  2:1267350/140‑1 (MQ=255)
                                                                                                                      tACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 <  2:563521/140‑1 (MQ=255)
                                                                                                                      tACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 <  1:605340/140‑1 (MQ=255)
                                                                                                                      tACGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 <  2:1021601/140‑1 (MQ=255)
                                                                                                                       aCGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAAt                >  1:933700/1‑140 (MQ=255)
                                                                                                                       aCGGACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGCAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAAt                >  1:69069/1‑140 (MQ=255)
                                                                                                                          gACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCct             <  2:1086206/140‑1 (MQ=255)
                                                                                                                          gACGTGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCct             >  1:47716/1‑140 (MQ=255)
                                                                                                                              tGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctctct         >  1:184995/1‑140 (MQ=255)
                                                                                                                              tGGGGGTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctctct         >  1:1035990/1‑140 (MQ=255)
                                                                                                                                ggggTTCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCg       >  1:1257613/1‑140 (MQ=255)
                                                                                                                                 gggTTCAAG‑TCCCCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCg       <  1:59153/140‑1 (MQ=255)
                                                                                                                                     tCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  1:84231/140‑1 (MQ=255)
                                                                                                                                     tCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:290668/140‑1 (MQ=255)
                                                                                                                                     tCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:770764/140‑1 (MQ=255)
                                                                                                                                     tCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  >  1:403931/1‑140 (MQ=255)
                                                                                                                                     tCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  1:1146625/140‑1 (MQ=255)
                                                                                                                                     tCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:184995/140‑1 (MQ=255)
                                                                                                                                     tCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  1:625244/140‑1 (MQ=255)
                                                                                                                                     tCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  >  2:367578/1‑140 (MQ=255)
                                                                                                                                     tCAAG‑TC‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:933700/140‑1 (MQ=255)
                                                                                                                                     tCAAG‑G‑‑CCCCCCCCCTGCGACCAAGAGTTTAAAGAATTGAACCAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:866458/140‑1 (MQ=38)
                                                                                                                                            |                                                                                                                                       
GGATTGTGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑T‑‑CCCCCCCCTCGC‑ACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982538‑3982809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑