Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,678,019	+G	coding (343/1512 nt)	yphE ←	putative ABC transporter ATP‑binding protein YphE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,015	1	.	G	100.0%	87.0 / NA	25	R116P (CGC→CCC)	yphE	putative ABC transporter ATP‑binding protein YphE
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (7/18);  total (7/18)

CCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCG  >  NC_000913/2677876‑2678147                                                                                                                                             |                                                                                                                                      ccGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTgcggc                                                                                                                                        >  2:1081738/1‑140 (MQ=255) ccGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATAAATCATGCCGTTgcggc                                                                                                                                        >  2:294936/1‑140 (MQ=255)     gcAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGcggc‑ggcg                                                                                                                                   >  1:1274041/1‑138 (MQ=255)     gcAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑gggg                                                                                                                                   >  2:28792/1‑140 (MQ=255)     gcAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑gggg                                                                                                                                   <  1:1172705/140‑1 (MQ=255)        aTTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑gggggcc                                                                                                                                <  2:1075949/140‑4 (MQ=255)         tttCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑gggggcca                                                                                                                               <  2:149832/140‑5 (MQ=255)         tttCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑gggggcca                                                                                                                               <  2:628009/140‑5 (MQ=255)              accaGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGAc                                                                                                                          <  2:563649/140‑1 (MQ=255)               ccaGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATAAATCATGCCGTTGCGGCGGGGGCCA‑CTGAcc                                                                                                                         <  1:294936/140‑1 (MQ=255)                 aGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGa                                                                                                                       <  1:28792/140‑1 (MQ=255)                 aGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGa                                                                                                                       <  1:795661/140‑1 (MQ=255)                      ctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCag                                                                                                                  <  1:1228491/140‑1 (MQ=255)                             gcCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGACATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGtttt                                                                                                           <  1:119373/140‑1 (MQ=255)                               cGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTcc                                                                                                         <  2:625458/140‑1 (MQ=255)                                 ggCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCGTTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGc                                                                                                       <  1:637518/140‑1 (MQ=255)                                  gCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGcc                                                                                                      <  1:1343877/140‑1 (MQ=255)                                      aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CCGACCCCGGGAGAGGGTTTTCGCCAccc                                                                                                  >  2:1126154/1‑138 (MQ=255)                                        cGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTc                                                                                                <  1:1126154/140‑1 (MQ=255)                                             aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCCCTGTCAGccc                                                                                           >  1:194548/1‑140 (MQ=255)                                                         cAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACt                                                                               <  2:408190/140‑1 (MQ=255)                                                             aCTAACGCCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAAc                                                                           <  1:1204996/140‑1 (MQ=255)                                                                 aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGGCA‑CCGACCCAGGCAGAGGGTTTCCGCCACCGTCAGCCCCTCCACCAGACTTAAATCCt                                                                       >  1:925572/1‑140 (MQ=255)                                                                 aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCC‑CCGACCCAGGCAGAGGGTTTTCCCCCCCGTCAGCCCCTCCCCCCCACCTAAATccc                                                                       >  2:538956/1‑139 (MQ=255)                                                                               cgcTTGTAAGGAACGTTGGGAATCTAGCGCCATTTGAAGGTAATCAATCATCCCGTTGCGGC‑GGGGGTACCTGCCCGTGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCgcgcgc                                                         <  2:925572/140‑1 (MQ=255)                                                                               cgcCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCgcgcgc                                                         <  2:1233731/140‑1 (MQ=255)                                                                                       ggCACCGTTGGTGCTCCTGCGCCATTTGTAGGTACTCAGTCATGCCGTGGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCATCCCTTCCACCAGACGTAACTCCTGATAAACCGCGCGCACCCCCAg                                                 <  2:800250/139‑1 (MQ=255)                                                                                                         gcgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGGCC‑CTGAGCCAGGGAGAGGGTTTCCGCCGCTCTCCGCCCCTCCCCCAGACTTAATTCCCGATAAAACACGGCCACCCCCCGTTCAGCGGCACAGCGAgg                               >  2:874209/1‑139 (MQ=255)                                                                                                         gcgcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGt                               <  1:538956/140‑1 (MQ=255)                                                                                                           gcCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CCGGCCGAGGCAGAGGGTTTTCCCCACTGTCAGCCCTTCCCCCAGACTTTAATCCTTTTAAACCGCGCCCACCCCCCGCTCCGCGGCGCCGCGAggcg                             >  2:600128/1‑137 (MQ=255)                                                                                                              acaggCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCg                          <  1:102270/136‑1 (MQ=255)                                                                                                                    aGGTAATCAATCATGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCtt                    <  1:874209/140‑1 (MQ=255)                                                                                                                         atcaatcaTGCCGTTGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCa               <  2:194548/140‑1 (MQ=255)                                                                                                                             atcaTGCCGTTGCGGCGGGGGCCC‑CCGACCCCGGGAGAGGGTTTTCGCCCCCGTCAACCCCTCCCCCCGACCTAAATCCCGATAAAACCCCCGCACCCCCCGGTCAGCGGCGCGGCGAGGCAGCGGAGCTTCGTCAccgt           >  2:102270/1‑138 (MQ=255)                                                                                                                                      ttGCGGCGGGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTcgcg  <  1:792/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                      CCGCGCAATTTCCACCAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGC‑GGGGCCA‑CTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCG  >  NC_000913/2677876‑2678147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑