Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	54,645	C→T	A20T (GCT→ACT)	surA ←	peptidyl‑prolyl cis‑trans isomerase SurA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	54,645	0	C	T	100.0%	275.6 / NA	78	A20T (GCT→ACT)	surA	peptidyl‑prolyl cis‑trans isomerase SurA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/34);  total (44/34)

CGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACAAAGTGTTTTGATACGGCAGAAT  >  NC_000913/54508‑54781                                                                                                                                          |                                                                                                                                         cgtcgcgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGa                                                                                                                                        >  2:425336/1‑140 (MQ=255)    cgcgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAAc                                                                                                                                     <  1:582949/140‑1 (MQ=255)       gtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTgg                                                                                                                                  <  2:1166683/140‑1 (MQ=255)          atcaGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTAt                                                                                                                               <  2:1298232/140‑1 (MQ=255)           tcaGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTAtt                                                                                                                              >  2:943490/1‑140 (MQ=255)           tcaGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTAtt                                                                                                                              >  1:518550/1‑140 (MQ=255)           tcaGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCAGGGGGGCAGTGAAACTGGTAtt                                                                                                                              <  1:955137/140‑1 (MQ=255)                 aGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGAt                                                                                                                        <  2:113277/140‑1 (MQ=255)                   ttGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCa                                                                                                                      >  2:95533/1‑140 (MQ=255)                     gctgcCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATg                                                                                                                    >  1:178319/1‑140 (MQ=255)                     gctgcCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATg                                                                                                                    >  1:645432/1‑140 (MQ=255)                      ctgcCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATgg                                                                                                                   >  1:694362/1‑140 (MQ=255)                      ctgcCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATgg                                                                                                                   <  2:694362/140‑1 (MQ=255)                         ccttgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGAt                                                                                                                        >  1:860484/1‑132 (MQ=255)                         ccttgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGa                                                                                                                <  2:477037/140‑1 (MQ=255)                         ccctgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGAt                                                                                                                        <  2:860484/129‑1 (MQ=255)                           ttgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATa                                                                                                              >  1:1290669/1‑140 (MQ=255)                              cctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCg                                                                                                           >  2:1329830/1‑140 (MQ=255)                              cctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCg                                                                                                           <  2:178319/140‑1 (MQ=255)                                 gAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCgaga                                                                                                        >  2:342697/1‑140 (MQ=255)                                       cGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAagcagc                                                                                                  >  1:1025937/1‑140 (MQ=255)                                        gTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCg                                                                                                 >  2:1308505/1‑140 (MQ=255)                                        gTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCg                                                                                                 <  2:510892/140‑1 (MQ=255)                                        gTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCg                                                                                                 <  1:220682/140‑1 (MQ=255)                                        gTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCg                                                                                                 >  2:312984/1‑140 (MQ=255)                                         ttCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGt                                                                                                >  2:126403/1‑140 (MQ=255)                                            aGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGtttt                                                                                             >  2:579921/1‑140 (MQ=255)                                                        tGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCGAGTTCTTCAt                                                                                 >  1:957980/1‑140 (MQ=255)                                                        tGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCAt                                                                                 >  1:30494/1‑140 (MQ=255)                                                        tGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCAt                                                                                 >  2:1031450/1‑140 (MQ=255)                                                                    tCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCa                                                                       <  2:1292261/138‑1 (MQ=255)                                                                    tCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCa                                                                       >  1:1292261/1‑138 (MQ=255)                                                                    tCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCAtt                                                                     >  1:631568/1‑140 (MQ=255)                                                                    tCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCAtt                                                                     >  2:1109019/1‑140 (MQ=255)                                                                    tCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTGTTCCAGTTCTTCATACTTTTTCCAtt                                                                     >  1:979590/1‑140 (MQ=255)                                                                          tCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAAtt                                                               >  1:5423/1‑140 (MQ=255)                                                                           cGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTa                                                              >  1:1089834/1‑140 (MQ=255)                                                                            gCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTaa                                                             >  2:435392/1‑140 (MQ=255)                                                                             cTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAAc                                                            >  1:962798/1‑140 (MQ=255)                                                                                 ccAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCa                                                        <  1:774519/140‑1 (MQ=255)                                                                                  cAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCAc                                                       <  2:1025937/140‑1 (MQ=255)                                                                                  cAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCAc                                                       <  1:425336/140‑1 (MQ=255)                                                                                   aGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACt                                                      <  1:1301471/140‑1 (MQ=255)                                                                                   aGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACt                                                      <  2:1089834/140‑1 (MQ=255)                                                                                          cgCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAtt                                               >  2:1386905/1‑140 (MQ=255)                                                                                          cgCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAtt                                               >  2:1370904/1‑140 (MQ=255)                                                                                          cgCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAtt                                               >  1:1370374/1‑140 (MQ=255)                                                                                          cgCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAtt                                               >  2:1288843/1‑140 (MQ=255)                                                                                          cgCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAtt                                               >  1:755572/1‑140 (MQ=255)                                                                                          cgCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAtt                                               >  2:1213541/1‑140 (MQ=255)                                                                                          cgCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAtt                                               >  1:778593/1‑140 (MQ=255)                                                                                          cgCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAtt                                               >  1:263443/1‑140 (MQ=255)                                                                                             cGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACg                                            <  1:1329830/140‑1 (MQ=255)                                                                                              gTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGt                                           <  2:565793/140‑1 (MQ=255)                                                                                              gTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGt                                           <  1:1109019/140‑1 (MQ=255)                                                                                                 aTTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGt                                        <  2:30494/140‑1 (MQ=255)                                                                                                   tGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTaa                                      <  1:943490/140‑1 (MQ=255)                                                                                                    gacgacGGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTaaa                                     >  2:783044/1‑140 (MQ=255)                                                                                                         cgGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATcaac                                <  1:913431/140‑1 (MQ=255)                                                                                                         cgGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATcaac                                <  2:778593/140‑1 (MQ=255)                                                                                                         cgGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATcaac                                >  1:1333801/1‑140 (MQ=255)                                                                                                         cgGCTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATcaac                                >  2:690214/1‑140 (MQ=255)                                                                                                            cTGCGACTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACaaa                             >  2:208049/1‑140 (MQ=255)                                                                                                                 aCTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATcaca                        <  1:1386905/140‑1 (MQ=255)                                                                                                                 aCTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATcaca                        <  2:755572/140‑1 (MQ=255)                                                                                                                 aCTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATcaca                        <  2:645432/140‑1 (MQ=255)                                                                                                                  cTTTATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACaa                       <  1:126403/140‑1 (MQ=255)                                                                                                                   tttATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACaaa                      <  2:5423/140‑1 (MQ=255)                                                                                                                   tttATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACaaa                      <  2:518550/140‑1 (MQ=255)                                                                                                                   tttATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACaaa                      <  1:783044/140‑1 (MQ=255)                                                                                                                    ttATCGACTACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACAAAg                     <  2:1370374/140‑1 (MQ=255)                                                                                                                            tACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATcaca                        <  2:61563/129‑1 (MQ=255)                                                                                                                            tACCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATcaca                        >  1:61563/1‑129 (MQ=255)                                                                                                                             aCCTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACAAAGTGTTTTGAt            <  1:564625/140‑1 (MQ=255)                                                                                                                              ccTGGGGGGCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACAAAGTGTTTTGATa           <  2:1290669/140‑1 (MQ=255)                                                                                                                                   ggggCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACAAAGTGTTTTGATACGGCa      >  1:482709/1‑140 (MQ=255)                                                                                                                                     ggCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACAAAGTGTTTTGATACGGCAGa    >  1:1054508/1‑140 (MQ=255)                                                                                                                                     ggCAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACAAAGTGTTTTGATACGGCAGa    >  1:593481/1‑140 (MQ=255)                                                                                                                                       cAGTGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACAAAGTGTTTTGATACGGCAGAAt  >  2:990057/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         CGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATTACGTGGTAAATCAACAAATCACAAAGTGTTTTGATACGGCAGAAT  >  NC_000913/54508‑54781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑