Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	426,744	T→C	V203A (GTA→GCA)	tgt →	tRNA‑guanine transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	426,744	0	T	C	100.0%	196.4 / NA	55	V203A (GTA→GCA)	tgt	tRNA‑guanine transglycosylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

TCCATGGAGATGTCTCTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGTAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGTTGGTAA  >  NC_000913/426608‑426879                                                                                                                                         |                                                                                                                                        tCCATGGAGATGTCTCTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGa                                                                                                                                      <  2:926794/140‑1 (MQ=255)    aTGGAGATGTCTCTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGatat                                                                                                                                   >  1:1246845/1‑140 (MQ=255)     tGGAGATGTCTCTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATc                                                                                                                                  <  1:1068422/140‑1 (MQ=255)         gaTGTCTCTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGtt                                                                                                                              >  2:911145/1‑140 (MQ=255)             tctcTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGa                                                                                                                          >  1:925288/1‑140 (MQ=255)              ctctGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGAt                                                                                                                         <  2:32822/140‑1 (MQ=255)               tctGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATg                                                                                                                        >  2:1150605/1‑140 (MQ=255)               tctGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATg                                                                                                                        >  1:1257985/1‑140 (MQ=255)                 tGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGc                                                                                                                      >  2:1175199/1‑140 (MQ=255)                      tGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGc                                                                                                                 <  2:296563/140‑1 (MQ=255)                       gggCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCt                                                                                                                >  2:1249033/1‑140 (MQ=255)                       gggCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCt                                                                                                                >  2:108941/1‑140 (MQ=255)                        ggCGAAGCGTAGCCGTGAGCGTTTAGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTg                                                                                                               >  2:468765/1‑140 (MQ=255)                            aaGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTcgg                                                                                                           <  1:757444/140‑1 (MQ=255)                                gTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGt                                                                                                       <  2:90700/140‑1 (MQ=255)                                  aGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCt                                                                                                     >  2:325828/1‑140 (MQ=255)                                  aGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCt                                                                                                     >  1:595213/1‑140 (MQ=255)                                         aGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCAGCGGTCTAGCtgtg                                                                                              >  1:867955/1‑140 (MQ=255)                                            gTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGt                                                                                           <  2:356756/140‑1 (MQ=255)                                                 gACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGcc                                                                                      <  2:1080674/140‑1 (MQ=255)                                                 gACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGcc                                                                                      <  1:1150605/140‑1 (MQ=255)                                                  aCAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCg                                                                                     <  1:911145/140‑1 (MQ=255)                                                    aGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGaa                                                                                   <  1:321719/140‑1 (MQ=255)                                                      tctcGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAg                                                                                 <  2:1084636/140‑1 (MQ=255)                                                      tctcGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAg                                                                                 <  2:794071/140‑1 (MQ=255)                                                      tctcGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAg                                                                                 >  1:900122/1‑140 (MQ=255)                                                           gAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGat                                                                            <  2:780059/140‑1 (MQ=255)                                                                   aaTGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTTTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCg                                                                    <  2:932594/140‑1 (MQ=255)                                                                      gcgcTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCAt                                                                 <  1:1181975/140‑1 (MQ=255)                                                                          tGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTg                                                             <  2:1193299/140‑1 (MQ=255)                                                                            tttGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGa                                                           <  2:595213/140‑1 (MQ=255)                                                                            tttGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGa                                                           <  2:282209/140‑1 (MQ=255)                                                                                      tcCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGc                                                 >  1:642736/1‑140 (MQ=255)                                                                                      tcCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGc                                                 >  1:304563/1‑140 (MQ=255)                                                                                      tcCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGc                                                 >  1:1280180/1‑140 (MQ=255)                                                                                      tcCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGGGAGCCGAAAACAGATATGCACCGCATTCTGGAGCATGTATGc                                                 >  2:649254/1‑140 (MQ=255)                                                                                                cGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTc                                       >  1:1054839/1‑140 (MQ=255)                                                                                                cGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTc                                       >  1:898245/1‑140 (MQ=255)                                                                                                cGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTc                                       >  1:1039177/1‑140 (MQ=255)                                                                                                cGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTc                                       >  1:507678/1‑140 (MQ=255)                                                                                                   ttACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCgg                                    <  1:108941/140‑1 (MQ=255)                                                                                                   ttACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCgg                                    <  1:1249033/140‑1 (MQ=255)                                                                                                     aCGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCa                                  >  1:619357/1‑140 (MQ=255)                                                                                                       gAAGATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTTGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGa                                <  2:1246845/140‑1 (MQ=255)                                                                                                          gATTTACGTGATATTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACaa                             <  1:1175199/140‑1 (MQ=255)                                                                                                                     tatTTCTGTTAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTAcc                  >  2:448784/1‑140 (MQ=255)                                                                                                                             ttAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGc          >  2:529783/1‑140 (MQ=255)                                                                                                                             ttAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGc          <  1:325828/140‑1 (MQ=255)                                                                                                                             ttAAAGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGc          >  2:786211/1‑140 (MQ=255)                                                                                                                               aaaGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGt        >  2:265254/1‑140 (MQ=255)                                                                                                                               aaaGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGt        >  1:773489/1‑140 (MQ=255)                                                                                                                               aaaGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGt        >  2:998330/1‑140 (MQ=255)                                                                                                                                 aGGTCTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGTTg      <  2:898245/140‑1 (MQ=255)                                                                                                                                     cTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGTTGGTaa  <  2:773489/140‑1 (MQ=255)                                                                                                                                     cTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGTTGGTaa  <  1:1227751/140‑1 (MQ=255)                                                                                                                                     cTGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGTTGGTaa  >  1:412152/1‑140 (MQ=255)                                                                                                                                      tGGCAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGTTGGTa   <  2:1299083/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCCATGGAGATGTCTCTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAATGCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAAGGTCTGGTAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCGCGTTACCTGATGGGCGTTGGTAA  >  NC_000913/426608‑426879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑