Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,524	T→C	V254A (GTA→GCA)	ygeY →	putative peptidase YgeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,524	0	T	C	100.0%	275.9 / NA	75	V254A (GTA→GCA)	ygeY	putative peptidase YgeY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/39);  total (36/39)

ATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGT  >  NC_000913/3009394‑3009660                                                                                                                                   |                                                                                                                                         aTTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAg                                                                                                                                 >  2:408533/1‑140 (MQ=255) aTTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAg                                                                                                                                 <  1:748024/140‑1 (MQ=255)    ttCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTg                                                                                                                              >  2:1390385/1‑140 (MQ=255)    ttCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTg                                                                                                                              <  2:109285/140‑1 (MQ=255)         aaTGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCATCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAg                                                                                                                         <  2:965400/140‑1 (MQ=255)         aaTGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAg                                                                                                                         <  2:1258924/140‑1 (MQ=255)           tGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGtt                                                                                                                       <  2:1052824/140‑1 (MQ=255)                   aTTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGa                                                                                                               >  1:66010/1‑140 (MQ=255)                   aTTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGa                                                                                                               >  1:804014/1‑140 (MQ=255)                     tCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGAcc                                                                                                             <  1:1041986/140‑1 (MQ=255)                      cTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACcg                                                                                                            <  1:983592/140‑1 (MQ=255)                         ggCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACcgtcg                                                                                                         >  1:482711/1‑140 (MQ=255)                                       cctctCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCg                                                                                           <  2:1153837/139‑1 (MQ=255)                                       aCTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCg                                                                                           <  2:1325019/140‑1 (MQ=255)                                       aCTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCg                                                                                           <  1:264071/140‑1 (MQ=255)                                       aCTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCg                                                                                           <  1:715311/140‑1 (MQ=255)                                        ctctCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCGCAAGCCGTTGCGCTGCAGCAGACAGCTGCGTAGTTTCAATCGACCGTCGTCTGACCTGGGGCGa                                                                                          >  2:1114792/1‑140 (MQ=255)                                        ctctCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGa                                                                                          <  1:685521/140‑1 (MQ=255)                                                aCGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTggg                                                                                  >  1:242030/1‑140 (MQ=255)                                                aCGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTggg                                                                                  >  1:1226504/1‑140 (MQ=255)                                                aCGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTggg                                                                                  >  2:1059432/1‑140 (MQ=255)                                                aCGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTggg                                                                                  <  1:685752/140‑1 (MQ=255)                                                   tCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAg                                                                               >  2:1288403/1‑140 (MQ=255)                                                     tGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGgc                                                                             >  2:749761/1‑140 (MQ=255)                                                       ggTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGgcgc                                                                           >  1:491027/1‑140 (MQ=255)                                                         ttATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGgcgcgc                                                                         <  2:491027/140‑1 (MQ=255)                                                         ttATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGgcgcgc                                                                         <  2:66010/140‑1 (MQ=255)                                                                 aaTTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGaa                                                                 >  1:976583/1‑140 (MQ=255)                                                                 aaTTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGaa                                                                 >  1:818504/1‑140 (MQ=255)                                                                      cTCGGCAAAGGCACCCTCACCGTTTCTCAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCcg                                                            >  2:909794/1‑140 (MQ=255)                                                                        cGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCcgcg                                                          <  2:804014/140‑1 (MQ=255)                                                                         ggCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCgcgc                                                         >  1:1210606/1‑140 (MQ=255)                                                                          gCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGcc                                                        <  1:146327/140‑1 (MQ=255)                                                                          gCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGcc                                                        <  2:242030/140‑1 (MQ=255)                                                                          gCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGcc                                                        <  2:1226504/140‑1 (MQ=255)                                                                            aaaGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcct                                                      <  1:1059432/140‑1 (MQ=255)                                                                            aaaGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcct                                                      <  1:1303393/140‑1 (MQ=255)                                                                              aGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctgc                                                    <  2:594620/140‑1 (MQ=255)                                                                              aGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctgc                                                    <  1:1218535/140‑1 (MQ=255)                                                                               ggCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctgcc                                                   <  1:1288403/140‑1 (MQ=255)                                                                                 cACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctgcctg                                                 <  2:818504/140‑1 (MQ=255)                                                                                 cACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCcctgcctg                                                 <  2:976583/140‑1 (MQ=255)                                                                                   cccTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCa                                               <  1:749761/140‑1 (MQ=255)                                                                                        aCCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACa                                          >  1:752400/1‑140 (MQ=255)                                                                                          cGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGa                                        >  2:185374/1‑140 (MQ=255)                                                                                           gTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGaa                                       >  1:1056014/1‑140 (MQ=255)                                                                                           gTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGaa                                       <  2:953969/140‑1 (MQ=255)                                                                                            tttCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGaaa                                      >  2:981574/1‑140 (MQ=255)                                                                                              tCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGc                                    >  1:1265604/1‑140 (MQ=255)                                                                                              tCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGc                                    <  2:482711/140‑1 (MQ=255)                                                                                                         cttcACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgttg                         <  2:784184/140‑1 (MQ=255)                                                                                                         cttcACATCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgttg                         <  1:1390385/140‑1 (MQ=255)                                                                                                              caTCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCt                    <  2:1210606/140‑1 (MQ=255)                                                                                                              caTCCCCAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCt                    <  2:366267/140‑1 (MQ=255)                                                                                                                 ccccAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgttg                         >  2:431466/1‑132 (MQ=255)                                                                                                                 ccccAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGgttg                         <  1:431466/132‑1 (MQ=255)                                                                                                                 ccccAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATg                 >  1:131891/1‑140 (MQ=255)                                                                                                                    cAAGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTAc              >  2:1380508/1‑140 (MQ=255)                                                                                                                      aGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGttt                      >  1:665552/1‑130 (MQ=255)                                                                                                                      aGCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGttt                      <  2:665552/130‑1 (MQ=255)                                                                                                                       gCCGTTGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAAc           <  1:777106/140‑1 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGttt                      >  2:884230/1‑125 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGttt                      >  1:533290/1‑125 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGttt                      <  2:533290/125‑1 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGttt                      <  1:884230/125‑1 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACg       >  2:1344219/1‑140 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACg       >  1:793924/1‑140 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACg       >  2:635195/1‑140 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACg       >  1:875524/1‑140 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACg       >  1:939857/1‑140 (MQ=255)                                                                                                                           ttGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACg       >  2:1158858/1‑140 (MQ=255)                                                                                                                            tGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGa      >  2:659309/1‑140 (MQ=255)                                                                                                                            tGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGa      >  2:932714/1‑140 (MQ=255)                                                                                                                            tGCGCTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGa      >  1:105102/1‑140 (MQ=255)                                                                                                                                cTGCAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGAccgt  <  2:939857/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         ATTTTCAAAATGGGTCCGATTCTTGGCGAATTACAAGAACTCTCCCAACGTCTGGGTTATGACGAATTCCTCGGCAAAGGCACCCTCACCGTTTCTGAAATCTTCTTCACATCCCCAAGCCGTTGCGCTGTAGCAGACAGCTGCGCAGTTTCAATCGACCGTCGTCTGACCTGGGGCGAAACCTGGGAAGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGT  >  NC_000913/3009394‑3009660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑