Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,117,047	T→C	N689S (AAC→AGC)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,117,047	0	T	C	100.0%	353.6 / NA	97	N689S (AAC→AGC)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (58/39);  total (58/39)

TAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT  >  NC_000913/3116913‑3117182                                                                                                                                       |                                                                                                                                        tAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCATGCAGCGGGATTGCATAAGGATCGCTAccc                                                                                                                                    <  2:318846/140‑1 (MQ=255)    gATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACGTCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCAcc                                                                                                                                 >  2:1043321/1‑140 (MQ=255)    gATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCAcc                                                                                                                                 >  1:435995/1‑140 (MQ=255)      ttGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTg                                                                                                                               >  1:778675/1‑140 (MQ=255)      ttGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTg                                                                                                                               >  2:932995/1‑140 (MQ=255)         cTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGt                                                                                                                            >  2:625988/1‑140 (MQ=255)         cTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGt                                                                                                                            >  1:929011/1‑140 (MQ=255)         cTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGt                                                                                                                            >  1:1119374/1‑140 (MQ=255)         cTCATCACGTCTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGt                                                                                                                            <  1:1302406/140‑1 (MQ=255)            atcaCGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTcac                                                                                                                         >  2:1364818/1‑140 (MQ=255)              caCGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACat                                                                                                                       >  1:564684/1‑140 (MQ=255)                     tCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCaaa                                                                                                                <  1:167415/140‑1 (MQ=255)                     tCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCaaa                                                                                                                <  1:179811/140‑1 (MQ=255)                      ccATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAg                                                                                                               <  2:435995/140‑1 (MQ=255)                          gATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGt                                                                                                           <  2:488764/140‑1 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCaaa                                                                                                                <  2:1399290/124‑1 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCaaa                                                                                                                >  1:572528/1‑124 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCaaa                                                                                                                <  2:572528/124‑1 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCaaa                                                                                                                >  1:1399290/1‑124 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagc                                                                                                >  1:1048521/1‑140 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagc                                                                                                >  1:671286/1‑140 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagc                                                                                                >  1:800022/1‑140 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagc                                                                                                >  1:887791/1‑140 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagc                                                                                                >  2:1254133/1‑140 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagc                                                                                                >  2:287852/1‑140 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagc                                                                                                >  2:795299/1‑140 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagc                                                                                                >  1:1133712/1‑140 (MQ=255)                                     aTCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATcagc                                                                                                >  1:1134325/1‑140 (MQ=255)                                        cACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCagcagc                                                                                             >  2:825005/1‑140 (MQ=255)                                         aCCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCg                                                                                            <  2:1133712/140‑1 (MQ=255)                                         aCCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCg                                                                                            <  1:795299/140‑1 (MQ=255)                                             ttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAt                                                                                        >  1:513647/1‑140 (MQ=255)                                             ttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAt                                                                                        >  2:396368/1‑140 (MQ=255)                                             ttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAt                                                                                        >  1:285586/1‑140 (MQ=255)                                             ttGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCAt                                                                                        >  2:1282043/1‑140 (MQ=255)                                                ttCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTc                                                                                     >  2:397056/1‑140 (MQ=255)                                                ttCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTc                                                                                     >  1:1197987/1‑140 (MQ=255)                                                 tCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTcc                                                                                    <  2:887791/140‑1 (MQ=255)                                                   aGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTg                                                                                  <  1:287852/140‑1 (MQ=255)                                                     aTAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGc                                                                                          >  1:44674/1‑130 (MQ=255)                                                     aTAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGc                                                                                          <  2:44674/130‑1 (MQ=255)                                                     aTAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTg                                                                                  >  1:1284697/1‑138 (MQ=255)                                                     aTAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTg                                                                                  <  2:1284697/138‑1 (MQ=255)                                                       aGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGa                                                                              <  1:625988/140‑1 (MQ=255)                                                         gCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATc                                                                            >  1:566176/1‑140 (MQ=255)                                                         gCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATc                                                                            <  1:100758/140‑1 (MQ=255)                                                          cGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCg                                                                           >  1:1264439/1‑140 (MQ=255)                                                            aTCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCgag                                                                         <  1:396368/140‑1 (MQ=255)                                                              cAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGAt                                                                       <  1:397056/140‑1 (MQ=255)                                                               aGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATc                                                                      >  2:659256/1‑140 (MQ=255)                                                                 aTCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGc                                                                    <  2:513647/140‑1 (MQ=255)                                                                  tCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGcc                                                                   <  2:388181/140‑1 (MQ=255)                                                                   cGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCa                                                                  >  1:398100/1‑140 (MQ=255)                                                                   cGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCa                                                                  >  1:1136394/1‑140 (MQ=255)                                                                      tCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAg                                                               >  2:507326/1‑140 (MQ=255)                                                                      tCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAg                                                               <  1:1259711/140‑1 (MQ=255)                                                                       cacaTCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGc                                                              <  2:1197987/140‑1 (MQ=255)                                                                            ccTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACtt                                                         <  2:1062230/140‑1 (MQ=255)                                                                            ccTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACtt                                                         <  1:907895/140‑1 (MQ=255)                                                                              tgctgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                       <  2:1119374/140‑1 (MQ=255)                                                                              tgctgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTaa                                                       >  1:1128557/1‑140 (MQ=255)                                                                                ctgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAg                                                               >  1:268686/1‑130 (MQ=255)                                                                                ctgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAg                                                               <  2:268686/130‑1 (MQ=255)                                                                                ctgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAt                                                     >  1:488782/1‑140 (MQ=255)                                                                                ctgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAACGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAAt                                                     >  1:442687/1‑140 (MQ=255)                                                                                 tgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATg                                                    <  2:1211492/140‑1 (MQ=255)                                                                                 tgAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATg                                                    <  2:566176/140‑1 (MQ=255)                                                                                   aGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCt                                                  <  2:671286/140‑1 (MQ=255)                                                                                    gTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                 >  2:525673/1‑140 (MQ=255)                                                                                    gTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTc                                                 >  1:619061/1‑140 (MQ=255)                                                                                          ttCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACaga                                           <  1:1282043/140‑1 (MQ=255)                                                                                             ggTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGAt                                        >  2:875062/1‑140 (MQ=255)                                                                                                  gCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCg                                   <  2:800022/140‑1 (MQ=255)                                                                                                   cTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGc                                  >  1:1227890/1‑140 (MQ=255)                                                                                                         tCTGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATc                            <  1:188364/140‑1 (MQ=255)                                                                                                           tGCACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCg                          >  1:388292/1‑140 (MQ=255)                                                                                                             cACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATc                            <  2:765061/136‑1 (MQ=255)                                                                                                             cACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATc                            >  1:765061/1‑136 (MQ=255)                                                                                                             cACGCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGa                        >  1:911972/1‑140 (MQ=255)                                                                                                               cccAGCGGAATTGATAAAGGACCGTT‑CCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                     <  2:1168917/138‑1 (MQ=255)                                                                                                                gCAGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATATCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATgg                     >  2:1290332/1‑140 (MQ=255)                                                                                                                  aGCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGaa                   >  1:962697/1‑140 (MQ=255)                                                                                                                   gCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAg                  >  1:398232/1‑140 (MQ=255)                                                                                                                   gCGGGATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAg                  >  1:255606/1‑140 (MQ=255)                                                                                                                     gggATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTc                >  2:442297/1‑140 (MQ=255)                                                                                                                       gATTGCATAAGGATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGa              <  1:621745/140‑1 (MQ=255)                                                                                                                              tAAGGATCGCTACCCACCTGAGGCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAgc       >  2:1292433/1‑140 (MQ=255)                                                                                                                                 ggATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCGGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCt    >  2:350920/1‑140 (MQ=255)                                                                                                                                 ggATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCt    >  1:179489/1‑140 (MQ=255)                                                                                                                                 ggATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCt    <  1:1290332/140‑1 (MQ=255)                                                                                                                                 ggATCGCTACACACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCt    >  1:1068688/1‑140 (MQ=255)                                                                                                                                  gATCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTg   <  2:1227890/140‑1 (MQ=255)                                                                                                                                   aTCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt  >  1:818841/1‑140 (MQ=255)                                                                                                                                   aTCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt  >  2:623410/1‑140 (MQ=255)                                                                                                                                   aTCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt  >  2:1384193/1‑140 (MQ=255)                                                                                                                                   aTCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt  <  1:875062/140‑1 (MQ=255)                                                                                                                                   aTCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt  <  2:285586/140‑1 (MQ=255)                                                                                                                                   aTCGCTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGt  <  1:932995/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TAAGATTGCTCATCACGTTTTCCATGATCAGCACCGATCCACCTTTGTTCAGATAGGCGATCAGATCGGTCACATCCTGCTGAGTCAGCTTCGGTTTGCTGGTATCTGCACGCAGCGGGATTGCATAAGGATCGTTACCCACCTGAGTCACATATTCAAAGCCGTTAAGGATCAGCAGCGGCATTTCCTGCGGATCGAGATCGCCATAGCTACTTAAATGCTCAACAGAGATGCCCGCAAAATCCGGATGGAAGTCGAACGCAGCGCTGT  >  NC_000913/3116913‑3117182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑