Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,222,157	G→A	R231Q (CGG→CAG)	ebgR →	DNA‑binding transcriptional repressor EbgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,222,157	0	G	A	100.0%	315.4 / NA	95	R231Q (CGG→CAG)	ebgR	DNA‑binding transcriptional repressor EbgR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/49);  total (46/49)

CCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCG  >  NC_000913/3222021‑3222288                                                                                                                                         |                                                                                                                                    ccTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGa                                                                                                                                  >  2:650730/1‑140 (MQ=255)  cTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGaa                                                                                                                                 <  1:552220/140‑1 (MQ=255)   tGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAg                                                                                                                                >  1:1122656/1‑140 (MQ=255)    ggCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGa                                                                                                                               <  1:741176/140‑1 (MQ=255)    ggCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGa                                                                                                                               <  1:283174/140‑1 (MQ=255)    ggCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGa                                                                                                                               <  1:224507/140‑1 (MQ=255)          gCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGGTATGAAATGGGAAAACAAATGCTGGCGCCGGAAGACTATcc                                                                                                                         >  2:340067/1‑140 (MQ=255)           cGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCg                                                                                                                        >  2:846894/1‑140 (MQ=255)           cGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCg                                                                                                                        <  1:650730/140‑1 (MQ=255)           cGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCg                                                                                                                        >  1:94974/1‑140 (MQ=255)            ggATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGa                                                                                                                       <  2:485303/140‑1 (MQ=255)            ggATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGa                                                                                                                       >  2:175011/1‑140 (MQ=255)                atTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGc                                                                                                                   <  2:1122656/140‑1 (MQ=255)                     tGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACtgt                                                                                                              >  1:146149/1‑140 (MQ=255)                     tGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACtgt                                                                                                              >  1:80154/1‑140 (MQ=255)                       aGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACtgttt                                                                                                            >  2:1352973/1‑140 (MQ=255)                         gTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGGTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACtgtttgt                                                                                                          >  2:407235/1‑140 (MQ=255)                           cGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTttgttg                                                                                                        <  1:299776/140‑1 (MQ=255)                           cGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTttgttg                                                                                                        >  2:269412/1‑140 (MQ=255)                            gCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGc                                                                                                       >  1:1342373/1‑140 (MQ=255)                            gCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGc                                                                                                       >  1:907751/1‑140 (MQ=255)                             ccTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCt                                                                                                      >  1:667734/1‑140 (MQ=255)                             ccTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGGTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCt                                                                                                      >  2:781318/1‑140 (MQ=255)                               tttGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTc                                                                                                    >  2:63793/1‑140 (MQ=255)                                  gCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGa                                                                                                 <  1:269412/140‑1 (MQ=255)                                  gCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGa                                                                                                 <  1:333403/140‑1 (MQ=255)                                  gCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCATCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGa                                                                                                 <  2:485651/140‑1 (MQ=255)                                   cGGAATATGGCCGACTGAAACGAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGAt                                                                                                <  1:1177278/140‑1 (MQ=255)                                   cGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGAt                                                                                                <  1:751387/140‑1 (MQ=255)                                           ggCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGc                                                                                        <  1:78315/140‑1 (MQ=255)                                             ccGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTa                                                                                      <  1:367461/140‑1 (MQ=255)                                                aCTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTa                                                                                      >  1:399167/1‑137 (MQ=255)                                                aCTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTa                                                                                      <  2:399167/137‑1 (MQ=255)                                                aCTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCg                                                                                   >  2:643493/1‑140 (MQ=255)                                                   gAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCg                                                                                <  1:455621/140‑1 (MQ=255)                                                      aCAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTAc                                                                             >  1:1011856/1‑140 (MQ=255)                                                            ggTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCggg                                                                       <  1:637330/140‑1 (MQ=255)                                                            ggTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCggg                                                                       <  1:175011/140‑1 (MQ=255)                                                               aCGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCaa                                                                    >  2:938023/1‑140 (MQ=255)                                                                cgcgAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAAt                                                                   >  2:1001943/1‑140 (MQ=255)                                                                   gAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCa                                                                <  1:1001943/140‑1 (MQ=255)                                                                        ggATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                           <  2:907751/140‑1 (MQ=255)                                                                        ggATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                           <  1:846894/140‑1 (MQ=255)                                                                        ggATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                           >  2:122071/1‑140 (MQ=255)                                                                        ggATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                           >  1:252759/1‑140 (MQ=255)                                                                          atatCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGa                                                         <  2:252759/140‑1 (MQ=255)                                                                             tCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGc                                                      <  2:1011856/140‑1 (MQ=255)                                                                               tGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCt                                                    >  2:378805/1‑140 (MQ=255)                                                                                 gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGa                                                  >  2:975858/1‑140 (MQ=255)                                                                                 gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGa                                                  >  2:789581/1‑140 (MQ=255)                                                                                 gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGa                                                  >  2:139868/1‑140 (MQ=255)                                                                                 gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGa                                                  >  2:1079928/1‑140 (MQ=255)                                                                                 gcgcGGCGGTTTTTCCAGTTCGTCGGGGTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCCAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGa                                                  >  2:875450/1‑140 (MQ=255)                                                                                  cgcgGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGaa                                                 <  2:667734/140‑1 (MQ=255)                                                                                   gcggcgGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGa                                                         <  1:194453/131‑1 (MQ=255)                                                                                   gcggcgGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGa                                                         >  2:194453/1‑131 (MQ=255)                                                                                   gcggcgGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAAc                                                >  1:871128/1‑140 (MQ=255)                                                                                    cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa                                               <  2:94974/140‑1 (MQ=255)                                                                                    cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa                                               <  2:927760/140‑1 (MQ=255)                                                                                    cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa                                               <  1:1368775/140‑1 (MQ=255)                                                                                     ggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACAt                                              >  2:17468/1‑140 (MQ=255)                                                                                      gcggTTTTTTCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATc                                             >  2:1034138/1‑140 (MQ=255)                                                                                       cggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                           >  2:497884/1‑125 (MQ=255)                                                                                       cggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                           <  1:497884/125‑1 (MQ=255)                                                                                       cggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATcc                                            <  1:139868/140‑1 (MQ=255)                                                                                       cggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACCATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATcc                                            >  2:74328/1‑140 (MQ=255)                                                                                         gTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCca                                          <  1:224021/140‑1 (MQ=255)                                                                                           ttttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCcaca                                        >  2:1095858/1‑140 (MQ=255)                                                                                           ttttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCcaca                                        <  1:340067/140‑1 (MQ=255)                                                                                           ttttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCcaca                                        <  1:1352973/140‑1 (MQ=255)                                                                                           ttttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACCATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCcaca                                        <  1:74328/140‑1 (MQ=255)                                                                                                 aGTTCGTCGGGGTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGatat                                  >  2:714809/1‑140 (MQ=255)                                                                                                        cgGGTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCtt                           >  2:534069/1‑140 (MQ=255)                                                                                                          ggTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTAt                         <  2:146149/140‑1 (MQ=255)                                                                                                           gTTATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATc                        <  1:63793/140‑1 (MQ=255)                                                                                                             tATGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAg                      >  2:916530/1‑140 (MQ=255)                                                                                                               tGAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCg                    <  1:1034138/140‑1 (MQ=255)                                                                                                                 aaCTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGtt                  <  1:299937/140‑1 (MQ=255)                                                                                                                  aCTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTa                 <  1:122071/140‑1 (MQ=255)                                                                                                                  aCTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTa                 <  1:171916/140‑1 (MQ=255)                                                                                                                    tGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc               <  1:120550/140‑1 (MQ=255)                                                                                                                    tGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc               <  2:80154/140‑1 (MQ=255)                                                                                                                    tGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc               <  1:405484/140‑1 (MQ=255)                                                                                                                    tGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGACCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc               >  2:516054/1‑140 (MQ=255)                                                                                                                      gCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa             >  2:553720/1‑140 (MQ=255)                                                                                                                      gCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa             >  1:656571/1‑140 (MQ=255)                                                                                                                           aCAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCa                       >  2:531768/1‑125 (MQ=255)                                                                                                                           aCAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCa                       <  1:531768/125‑1 (MQ=255)                                                                                                                           aCAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATcc        >  2:237156/1‑140 (MQ=255)                                                                                                                            cAAATGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATccc       <  1:237156/140‑1 (MQ=255)                                                                                                                              aaTGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCa     >  2:581949/1‑140 (MQ=255)                                                                                                                                tGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAcc   >  1:221402/1‑140 (MQ=255)                                                                                                                                tGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAcc   >  2:1354341/1‑140 (MQ=255)                                                                                                                                tGCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCAcc   >  1:44219/1‑140 (MQ=255)                                                                                                                                 gCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcg  <  1:553720/140‑1 (MQ=255)                                                                                                                                 gCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcg  <  1:581949/140‑1 (MQ=255)                                                                                                                                 gCTGGCGCAGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcg  <  1:938023/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CCTGGCAAGGCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCG  >  NC_000913/3222021‑3222288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑