Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	440,973	A→G	G43G (GGT→GGC)	ispA ←	geranyl diphosphate/farnesyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	440,973	0	A	G	100.0%	173.8 / NA	49	G43G (GGT→GGC)	ispA	geranyl diphosphate/farnesyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/22);  total (27/22)

TGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTAC  >  NC_000913/440838‑441104                                                                                                                                        |                                                                                                                                    tGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCta                                                                                                                                 >  2:1288611/1‑140 (MQ=255)    cGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCtaata                                                                                                                              <  2:271251/140‑1 (MQ=255)      gTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTaataat                                                                                                                            <  2:345364/140‑1 (MQ=255)       tAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCtaata                                                                                                                              >  1:43358/1‑137 (MQ=255)       tAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCtaata                                                                                                                              <  2:43358/137‑1 (MQ=255)                  gAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGc                                                                                                                <  2:937326/140‑1 (MQ=255)                     ttAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCAAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATg                                                                                                             >  2:760459/1‑140 (MQ=255)                             tAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGAc                                                                                                     >  2:575271/1‑140 (MQ=255)                                   tGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACggg                                                                                               >  2:257648/1‑140 (MQ=255)                                    ggATACACTCAACGGCGGCAGCGGGTGCGTCCAGTGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGa                                                                                              <  1:592276/140‑1 (MQ=255)                                    ggATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGa                                                                                              <  1:575271/140‑1 (MQ=255)                                    ggATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGa                                                                                              <  1:640140/140‑1 (MQ=255)                                    ggATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGa                                                                                              <  1:985639/140‑1 (MQ=255)                                     gATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAg                                                                                             >  1:224521/1‑140 (MQ=255)                                     gATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAg                                                                                             >  1:694675/1‑140 (MQ=255)                                           cTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGa                                                                                              >  1:1320049/1‑133 (MQ=255)                                           cTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGa                                                                                              <  2:1320049/133‑1 (MQ=255)                                           cTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCt                                                                                       >  2:554184/1‑140 (MQ=255)                                               aCGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGaaa                                                                                   <  2:224521/140‑1 (MQ=255)                                                   cggcAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTTGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGc                                                                               >  2:972009/1‑140 (MQ=255)                                                   cggcAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGc                                                                               >  1:305169/1‑140 (MQ=255)                                                   cggcAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGc                                                                               >  1:42262/1‑140 (MQ=255)                                                      cAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGt                                                                            >  2:378727/1‑140 (MQ=255)                                                        gCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTgg                                                                          >  1:334003/1‑140 (MQ=255)                                                        gCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTgg                                                                          <  2:33361/140‑1 (MQ=255)                                                               cGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATa                                                                   <  1:972009/140‑1 (MQ=255)                                                               cGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATa                                                                   >  2:1042605/1‑140 (MQ=255)                                                                gTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATaa                                                                  >  2:1176361/1‑140 (MQ=255)                                                                          tttGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAg                                                        >  1:1000249/1‑140 (MQ=255)                                                                            tgtgCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAgcg                                                      <  2:42262/140‑1 (MQ=255)                                                                                      ccGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGc                                            <  1:1290059/140‑1 (MQ=255)                                                                                      ccGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGc                                            <  1:257648/140‑1 (MQ=255)                                                                                          aCATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGc                                        >  2:556366/1‑140 (MQ=255)                                                                                                   cGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAg                               <  2:1000249/140‑1 (MQ=255)                                                                                                      tGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCt                            >  1:1077428/1‑140 (MQ=255)                                                                                                      tGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCt                            >  2:1314503/1‑140 (MQ=255)                                                                                                      tGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCt                            >  2:504903/1‑140 (MQ=255)                                                                                                           tAAACCAGGAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAg                       >  2:883671/1‑140 (MQ=255)                                                                                                                  ggAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTCTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTtgctgc                >  2:741566/1‑140 (MQ=255)                                                                                                                   gAAAGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCg               <  1:556366/140‑1 (MQ=255)                                                                                                                     aaGGTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGa             <  2:1331348/140‑1 (MQ=255)                                                                                                                        gTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAg          >  2:551644/1‑140 (MQ=255)                                                                                                                        gTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAg          >  2:543215/1‑140 (MQ=255)                                                                                                                        gTCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAg          >  1:759730/1‑140 (MQ=255)                                                                                                                         tCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGt         <  1:760459/140‑1 (MQ=255)                                                                                                                         tCGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGt         <  2:759730/140‑1 (MQ=255)                                                                                                                          cGCAGGCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTc        >  1:694376/1‑140 (MQ=255)                                                                                                                              ggCGCTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCAtt    <  1:1099435/140‑1 (MQ=255)                                                                                                                                cgcTTACCGCCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTAc  <  2:305169/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TGCCGGTAAATCATCATGAATTAATGAGTAAGCGTGGATACACTCAACGGCGGCAGCGGGTGCGTCCAGCGTGTTTGTGCTAACGCCGAACATATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGTCCATTAC  >  NC_000913/440838‑441104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑