Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,461,863	C→T	I132I (ATC→ATT)	gspH →	Type II secretion system protein GspH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,461,863	0	C	T	100.0%	264.0 / NA	74	I132I (ATC→ATT)	gspH	Type II secretion system protein GspH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/38);  total (36/38)

GCTGGGTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATCACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATG  >  NC_000913/3461727‑3461994                                                                                                                                         |                                                                                                                                    gcTGGGTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACg                                                                                                                                  <  1:696670/140‑1 (MQ=255)   tGGGTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGcc                                                                                                                                <  1:698525/140‑1 (MQ=255)    gggTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGccc                                                                                                                               >  1:90500/1‑140 (MQ=255)     ggTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGccc                                                                                                                               >  2:105905/1‑139 (MQ=255)     ggTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGccc                                                                                                                               <  1:105905/139‑1 (MQ=255)     ggTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCt                                                                                                                              <  1:190081/140‑1 (MQ=255)     ggTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCt                                                                                                                              <  1:207635/140‑1 (MQ=255)               cAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTttgtt                                                                                                                    >  1:767238/1‑140 (MQ=255)                aagaagATGTCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTttgttg                                                                                                                   <  2:1281469/140‑1 (MQ=255)                aagaagATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTttgttg                                                                                                                   <  2:767238/140‑1 (MQ=255)                    agaTGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATgg                                                                                                               >  2:1157721/1‑140 (MQ=255)                    agaTGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATgg                                                                                                               >  2:648505/1‑140 (MQ=255)                     gaTGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGc                                                                                                              >  2:515353/1‑140 (MQ=255)                      aTGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCt                                                                                                             <  1:999413/140‑1 (MQ=255)                       tGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCt                                                                                                             <  2:467316/139‑1 (MQ=255)                       tGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCt                                                                                                             >  1:467316/1‑139 (MQ=255)                       tGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTa                                                                                                            >  2:1285335/1‑140 (MQ=255)                       tGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTa                                                                                                            >  1:841805/1‑140 (MQ=255)                       tGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTa                                                                                                            >  1:1102265/1‑140 (MQ=255)                         ccGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAAc                                                                                                          >  2:686891/1‑140 (MQ=255)                          cGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACg                                                                                                         >  1:700347/1‑140 (MQ=255)                          cGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACg                                                                                                         >  2:1231311/1‑140 (MQ=255)                            cAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGcc                                                                                                       >  1:103263/1‑140 (MQ=255)                            cAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGcc                                                                                                       >  1:403389/1‑140 (MQ=255)                                 gagaGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGAc                                                                                                  >  1:813717/1‑140 (MQ=255)                                   gagCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTGCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCg                                                                                                <  2:563175/140‑1 (MQ=255)                                    agCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCgg                                                                                               <  1:515353/140‑1 (MQ=255)                                      ccAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTg                                                                                             >  1:902337/1‑140 (MQ=255)                                         aaaTGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGc                                                                                          <  2:617792/140‑1 (MQ=255)                                           aTGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCg                                                                                        <  2:700347/140‑1 (MQ=255)                                               cTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTcc                                                                                    <  1:63968/140‑1 (MQ=255)                                               cTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTcc                                                                                    <  1:1236220/140‑1 (MQ=255)                                               cTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTcc                                                                                    <  1:413117/140‑1 (MQ=255)                                               cTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTcc                                                                                    <  1:834598/140‑1 (MQ=255)                                                    aTGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACg                                                                               <  2:102265/140‑1 (MQ=255)                                                        agaaCTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAg                                                                           <  1:884049/140‑1 (MQ=255)                                                         gaaCTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGt                                                                          <  1:708529/140‑1 (MQ=255)                                                         gaaCTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGt                                                                          <  1:570030/140‑1 (MQ=255)                                                         gaaCTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGt                                                                          <  2:103263/140‑1 (MQ=255)                                                         gaaCTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGt                                                                          <  1:1387625/140‑1 (MQ=255)                                                             tctcAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGt                                                                          <  2:658524/136‑1 (MQ=255)                                                             tctcAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGt                                                                          >  1:658524/1‑136 (MQ=255)                                                                       tCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCAt                                                            <  2:1102265/140‑1 (MQ=255)                                                                       tCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCAt                                                            <  2:90500/140‑1 (MQ=255)                                                                       tCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCAt                                                            <  2:922640/140‑1 (MQ=255)                                                                       tCTTCAGCCATTTAAACCTGATGACAGCAACCAACCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCAt                                                            <  2:114618/140‑1 (MQ=255)                                                                        cTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGATGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATg                                                           <  2:404215/140‑1 (MQ=255)                                                                           cAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGcc                                                        >  1:111707/1‑140 (MQ=255)                                                                            aGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGccc                                                       >  1:848612/1‑140 (MQ=255)                                                                                aTTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTgg                                                   >  2:224170/1‑140 (MQ=255)                                                                                  ttAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGAt                                                 >  2:324515/1‑140 (MQ=255)                                                                                        cTGATGACAGCAACCAGCCACAGGTTGTGATTCTTGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACg                                           <  1:802701/140‑1 (MQ=255)                                                                                        cTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACg                                           <  2:848612/140‑1 (MQ=255)                                                                                        cTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACg                                           <  1:1041570/140‑1 (MQ=255)                                                                                         tgatgaCAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGc                                          <  2:111707/140‑1 (MQ=255)                                                                                           atgaCAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTc                                        >  2:1227134/1‑140 (MQ=255)                                                                                           atgaCAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTc                                        >  2:524588/1‑140 (MQ=255)                                                                                            tgaCAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACg                                           >  2:456941/1‑136 (MQ=255)                                                                                            tgaCAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACg                                           <  1:456941/136‑1 (MQ=255)                                                                                            tgaCAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCg                                       <  2:403389/140‑1 (MQ=255)                                                                                            tgaCAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCg                                       <  2:1017279/140‑1 (MQ=255)                                                                                                     ccAGCCACAGGTTATGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATa                              >  2:489134/1‑140 (MQ=255)                                                                                                         ccACAGGTTGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCg                          >  1:440184/1‑140 (MQ=255)                                                                                                                ttGTGATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGa                   >  2:1161879/1‑140 (MQ=255)                                                                                                                   tgATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGaaca                <  1:1380094/140‑1 (MQ=255)                                                                                                                   tgATTCTGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGaaca                <  1:524588/140‑1 (MQ=255)                                                                                                                         tGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAAt          >  1:1311974/1‑140 (MQ=255)                                                                                                                         tGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAAt          >  2:398629/1‑140 (MQ=255)                                                                                                                         tGGCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAAt          >  2:616972/1‑140 (MQ=255)                                                                                                                          ggCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATc         >  2:180387/1‑140 (MQ=255)                                                                                                                          ggCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATc         <  2:440184/140‑1 (MQ=255)                                                                                                                           gCCGACGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCACAGATACCCGCCCATGAACAAACAATCa        >  2:1028852/1‑140 (MQ=255)                                                                                                                               aCGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGa    >  1:418418/1‑140 (MQ=255)                                                                                                                               aCGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGa    >  2:225392/1‑140 (MQ=255)                                                                                                                                cGGACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGATCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGAt   >  1:981216/1‑140 (MQ=255)                                                                                                                                 ggACAAATTACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATg  >  1:396690/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    GCTGGGTTCCATTACAAGAAGATGCCGCAGACGAGAGCCAAAATGACTGGGATGAAGAACTCTCAATTCATCTTCAGCCATTTAAACCTGATGACAGCAACCAGCCACAGGTTGTGATTCTGGCCGACGGACAAATCACGCCCTTCTCTTTGTTGATGGCTAACGCCGGGACCGGTGAGCCGCTCCTGACGTTAGTCTGTTCAGGCTCATGGCCCCTGGATCAAACGCTCGCCAGAGATACCCGCCCATGAACAAACAATCAGGGATG  >  NC_000913/3461727‑3461994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑